A5038117522- Monoclonal and Polyclonal Antibodies Research
- Peptidase Inhibition and Analysis
- SARS-CoV-2 and COVID-19 Research
- Electrochemical Analysis and Applications
- Advanced biosensing and bioanalysis techniques
- Cancer Immunotherapy and Biomarkers
- Synthetic Organic Chemistry Methods
- Organoboron and organosilicon chemistry
- Trace Elements in Health
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Molecular Sensors and Ion Detection
- Software Testing and Debugging Techniques
- Asymmetric Synthesis and Catalysis
- Chemical Synthesis and Reactions
- Protein purification and stability
- Immunodeficiency and Autoimmune Disorders
- Ubiquitin and proteasome pathways
- interferon and immune responses
- Cancer-related gene regulation
- Heavy Metal Exposure and Toxicity
- Respiratory viral infections research
- Microfluidic and Bio-sensing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- RNA and protein synthesis mechanisms
GigaGen (United States)
2020-2024
Grifols (United States)
2024
University of Colorado Boulder
2004-2018
Northwest University
2011-2014
Western Washington University
2010-2013
Fluorescent biosensors are important measurement tools for in vivo quantification of pH, concentrations metal ions and other analytes, physical parameters such as membrane potential. Both the development these sensors their implementation examining cellular heterogeneity requires technology measuring sorting cells based on fluorescence levels before after chemical or perturbations. We developed a droplet microfluidic platform screening separation cell populations basis response expressed...
Genetically encoded sensors based on fluorescence resonance energy transfer (FRET) are powerful tools for quantifying and visualizing analytes in living cells, when targeted to organelles have the potential define distribution of different parts cell. However, quantitative estimates analyte require rigorous systematic analysis sensor functionality locations. In this work, we establish methods critically evaluate performance carry out a side-by-side comparison three genetically platforms...
A streamlined synthesis of β-hydroxy ketone substrates has been developed to further investigate a recently discovered cooperative Lewis base-mediated intramolecular carbonyl hydrosilylation reaction. The features an enone β-borylation/oxidation sequence that proven be quite general and high-yielding. This allowed for additional investigations into the diastereoselectivity reaction through preparation important polyketide fragments.
In vitro affinity maturation of therapeutic monoclonal antibodies is commonly applied to achieve desired properties, such as improved binding kinetics and affinity. Currently there are no universally accepted protocols for generation variegated antibody libraries or selection thereof. Here, we performed using a yeast-based single-chain variable fragment (scFv) expression system compare two mutagenesis methods: random across the entire V(D)J region by error-prone PCR, novel combinatorial...
In this work, we developed PolyMap (polyclonal mapping), a high-throughput method for mapping protein-protein interactions. We demonstrated the of thousands antigen-antibody interactions between diverse antibody libraries isolated from convalescent and vaccinated COVID-19 donors set clinically relevant SARS-CoV-2 spike variants. identified over 150 antibodies with variety distinctive binding patterns toward antigen variants found broader profile, including targeting Omicron variant, in...
β-Hydroxyketones can be directly converted to cyclic disiloxanes using diphenylchlorosilane in the presence of imidazole and an amine base. The reaction is proposed proceed via a nucleophilic activation mechanism through chairlike transition state affording hydrosilylated products with high diastereoselectivity.
Conventionally, hyperimmune globulin drugs manufactured from pooled immunoglobulins vaccinated or convalescent donors have been used in treating infections where no treatment is available. This especially important multi-epitope neutralization required to prevent the development of immune-evading viral mutants that can emerge upon with monoclonal antibodies. Using microfluidics, flow sorting, and a targeted integration cell line, first-in-class recombinant therapeutic against SARS-CoV-2...
ABSTRACT Anti-CTLA-4 antibodies such as ipilimumab were among the first immune-oncology agents to show significantly improved outcomes for patients. However, existing anti-CTLA-4 therapies fail induce a response in majority of patients and can severe, immune-related adverse events. It has been assumed that checkpoint inhibition, i.e., blocking interaction between CTLA-4 its ligands, is primary mechanism action ipilimumab. In this study we present evidence inhibition not efficacy antibodies....
ABSTRACT Plasma-derived polyclonal antibodies are polyvalent drugs used for many important clinical indications that require modulation of multiple drug targets simultaneously, including emerging infectious disease and transplantation. However, plasma-derived suffer problems, low potency, impurities, constraints on supply, batch-to-batch variation. In this study, we demonstrated proofs-of-concept a technology uses microfluidics molecular genomics to capture diverse mammalian antibody...
Abstract The hydroxy ketones (II), (X), and (XII), generated from enones by a convenient procedure, are found to undergo base‐mediated hydrosilylation with high diastereoselectivity.
<h3>Background</h3> Anti-CTLA-4 antibodies, such as ipilimumab, were among the first immuno-oncology agents to provide significantly improved outcomes for patients. However, existing anti-CTLA-4 therapies fail induce a response in majority of patients, and can severe immune-related adverse events. It has been assumed that checkpoint inhibition, i.e., blocking interaction between CTLA-4 its B7 ligands (CD80 CD86), is primary mechanism action ipilimumab. Here we present non-clinical evidence...
Abstract Masked triene substrates undergo Ru‐catalyzed cross‐metathesis according to A) afford trienes after reductive elimination B).
Abstract Anti-CTLA-4 antibodies such as ipilimumab were among the first immuno-oncology agents to show significantly improved outcomes for patients. However, existing anti-CTLA-4 therapies fail induce a response in majority of patients and can severe, immune-related adverse events. It has been assumed that checkpoint inhibition, i.e., blocking interaction between CTLA-4 its ligands, is primary mechanism action ipilimumab. Here we present evidence inhibition may not be efficacy antibodies....