A5076807551- Microbial Natural Products and Biosynthesis
- Synthetic Organic Chemistry Methods
- Genomics and Phylogenetic Studies
- Enzyme Production and Characterization
- Chemical Synthesis and Analysis
- Synthesis and Catalytic Reactions
- Marine Sponges and Natural Products
- Carbohydrate Chemistry and Synthesis
South China Sea Institute Of Oceanology
2023-2025
Institute of Oceanology
2023-2025
Chinese Academy of Sciences
2023-2025
Cinnamoyl-containing nonribosomal peptides (CCNPs) constitute a unique family of natural products. The enzyme mechanism for the biaryl phenol coupling reaction bicyclic CCNPs remains unclear. Herein, we report discovery two new arabinofuranosylated cihanmycins (CHMs) A (1) and B (2) from Amycolatopsis cihanbeyliensis DSM 45679 identification CHM biosynthetic gene cluster (cih BGC) by heterologous expression in Streptomyces lividans SBT18 to afford CHMs C (3) D (4). structure 1 was confirmed...
A known polycyclic tetramate macrolactam (aburatubolactam C, 3) and three new ones (aburatubolactams D–F, 4–6, respectively) were isolated from the marine-derived Streptomyces sp. SCSIO 40070. The absolute configuration of 3 was established by X-ray analysis. combinatorial biosynthetic approach unveiled enzymes dictating formation distinct 5/5-type ring systems (such as C7–C14 cyclization AtlB1 in 5 C6–C13 AtlB2 6) aburatubolactams.
Bacterial tryptophan dimers comprise a large family of natural products with promising biological activities. Herein, we report the identification methyltransferase, SpmM1, which acts as gatekeeping enzyme to govern divergent biosynthesis spiroindimicins (SPMs) and indimicins (IDMs) in Streptomyces marincola SCSIO 03032. Bioinformatics, genetic, biochemical studies revealed that SpmM1-catalyzed carboxyl O-methylation enabled common precursor SPMs IDMs flux into spiro-forming pathway generate SPMs.
The antibacterial agents deoxynybomycin (DNM) and nybomycin (NM) have a unique tetracyclic structure featuring an angularly fused 4-oxazoline ring. Here, we report the identification of key enzymes responsible for forming ring in Embleya hyalina NBRC 13850 by comparative bioinformatics analysis biosynthetic gene clusters encoding structurally similar natural products DNM, deoxynyboquinone (DNQ), diazaquinomycins (DAQs). N-methyltransferase DnmS plays crucial role catalyzing N-dimethylation...
Tetronate antibiotics make up a growing family of natural products with wide variety biological activities. Herein, we report four new tetronates kongjuemycins (KJMs, 5-8) from coral-associated actinomycete Pseudonocardia kongjuensis SCSIO 11457, and the identification characterization KJM biosynthetic gene cluster (kjm) by heterologous expression, comparative genomic analysis, isotope labeling, knockout studies. The biosynthesis KJMs is demonstrated to harness diverse precursors primary...
Identifying new environmental resistance determinants is significant to combat rising antibiotic resistance. Herein we report the unexpected correlation of a lobophorin (LOB) resistance-related glycosidase KijX with host-dependent chemical diversity LOBs, by process glycosylation, deglycosylation and reglycosylation. homologues are widespread among bacteria, archaea fungi, encode same glycohydrolytic activity on LOBs. The crystal structure AcvX (a homologue) shows similar fold that glycoside...
Abstract Identifying new environmental resistance determinants is significant to combat rising antibiotic resistance. Herein we report the unexpected correlation of a lobophorin (LOB) resistance‐related glycosidase KijX with host‐dependent chemical diversity LOBs, by process glycosylation, deglycosylation and reglycosylation. homologues are widespread among bacteria, archaea fungi, encode same glycohydrolytic activity on LOBs. The crystal structure AcvX (a homologue) shows similar fold that...