A5038794714- Prenatal Screening and Diagnostics
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Parvovirus B19 Infection Studies
- Fetal and Pediatric Neurological Disorders
- Hepatitis B Virus Studies
- Synthesis and biological activity
- Synthesis and Characterization of Heterocyclic Compounds
- Metal complexes synthesis and properties
- Genomic variations and chromosomal abnormalities
- Chronic Kidney Disease and Diabetes
- Vitamin C and Antioxidants Research
- Cancer Research and Treatments
- Burn Injury Management and Outcomes
- Advanced biosensing and bioanalysis techniques
- Phytochemical compounds biological activities
- Sperm and Testicular Function
- Hepatitis Viruses Studies and Epidemiology
- Genetic Syndromes and Imprinting
- Ubiquitin and proteasome pathways
- Hepatitis C virus research
- Renal and related cancers
- Congenital Ear and Nasal Anomalies
- Cytokine Signaling Pathways and Interactions
- Autophagy in Disease and Therapy
Luohe Medical College
2017-2025
Brown University
2025
Shantou University
2016-2020
Shantou University Medical College
2016-2020
Abstract Polyethyleneimine (PEI), a cationic polymer vehicle, forms complex with DNA which then can carry anionic nucleic acids into eukaryotic cells. PEI‐based transfection is widely used for transient of plasmid DNA. The efficiency affected by numerous factors, including the way PEI/DNA prepared, ratio PEI to DNA, concentration storage conditions solutions, and more. Considering major influencing has been optimized improve its efficiency, reproducibility, consistency. This protocol...
Purpose To evaluate the clinical performance of expanded non-invasive prenatal testing (NIPT-plus) in screening for fetal chromosome aneuploidy and copy number variations (CNVs) among pregnant women with different risk factors to investigate how target population cell-free DNA may change NIPT-plus. Methods The data, test results, confirmatory invasive outcomes, follow-up results 6,220 who underwent NIPT-plus were re-viewed. indicators positive predictive value (PPV), rate (PR), specificity,...
Purpose To evaluate the clinical performance of expanded non-invasive prenatal testing (NIPT-plus) and compare its effectiveness in screening for chromosomal aneuploidies with that NIPT. Methods Screening results, confirmatory invasive follow-up data from pregnant women who underwent either NIPT (6792 cases) or NIPT-Plus (5237 at Luohe Central Hospital, China, January 2019 to June 2023 were collected. The positive predictive value (PPV), sensitivity, specificity, other indicators different...
Few cases of prenatal screening and diagnosis for NFIA-related disorders (OMIM # 613735) have been reported. Noninvasive testing (NIPT) has extensively utilized the fetal chromosomal abnormalities. Here, we report a case an disorder following NIPT result. A 24-year-old pregnant woman chose expanded noninvasive (NIPT-plus) abnormalities at 19 weeks 5 days gestation with singleton pregnancy. The results NIPT-plus revealed 0.9 Mb deletion in chromosome 1p31.3, including NFIA gene, which was...
Objective This study aims to assess the feasibility of detecting and diagnosing Duchenne muscular dystrophy (DMD) during prenatal screening for chromosome abnormalities using cell-free fetal DNA extracted from peripheral blood samples pregnant women. Study Design Two women identified as high risk through noninvasive testing (NIPT) underwent amniocentesis obtain cells. Subsequent chromosomal karyotyping was conducted, genomic cells copy number variation sequencing (CNV-Seq) analysis,...
Hepatitis B virus (HBV) infection may affect sperm motility in patients with HBV. HBV surface protein (HBs) decreases mitochondrial membrane potential, impairs and induces apoptotic-like changes human spermatozoa. However, little is known about how spermatozoa respond to reactive oxygen species (ROS; mainly peroxides) induced by HBs. In this study, HBs supraphysiological ROS levels reduced the formation of 2-cell embryos (obtained from hamster oocytes spermatozoa). a pre-apoptotic status...
In this paper, the association between polymorphisms of IFN-γ +874T/A (rs2430561), IFN-γR1 -56 T/C (rs2234711), +95 C/T (rs7749390), and -611A/G (rs 1327474) human papillomavirus (HPV) susceptibility was investigated in rural women from Luohe, Henan, China.A total 520 were enrolled including 260 with HPV infection mild dysplasia or less without infection. Single-nucleotide (SNPs) +874T/A, T/C, genotyped using TaqMan Pre-Designed SNP Genotyping Assays. Serum levels measured Human Quantikine...
<title>Abstract</title> Background Non-Invasive Prenatal Testing (NIPT) has gained extensive adoption worldwide for screening chromosomal abnormalities like trisomy 21. Nevertheless, controversies persist surrounding the efficacy of NIPT among diverse risk groups pregnant women and its capability to detect sex chromosome aneuploidies. This study evaluates performance various in detecting aneuploidies by retrospectively analyzing results 8,237 Luohe region China. Methods A total 8237 cases...
Context Four algorithms with relatively balanced complexity and accuracy in deep learning classification algorithm were selected for differential diagnosis of primary membranous nephropathy (PMN).