[Therapeutic mechanism of natural astaxanthin against renal clear cell carcinoma based on network pharmacology and bioinformatics].

• DOI: 10.12122/j.issn.1673-4254.2021.12.02
• Publication Date: 2021-12-20
• Authors: Junjie Gao; Dandan Yang; R Cao; Xueshan Pan; Jun Xia
• Keywords: Docking (animal)

To explore the molecular mechanism by which natural astaxanthin (AST) inhibits renal clear cell carcinoma (KIRC) based on network pharmacology and bioinformatics.PharmMapper database was used to retrieve targets of astaxanthin, TCGA identify differentially expressed genes (DEGs) in KIRC adjacent tissues. The target AST analyzed using Cytoscape software construct "drug-target" diagram. visual protein-protein interaction (PPI) constructed String database, GO enrichment analysis core performed. Single gene bioinformatics performed verify screened AST, namely placental growth factor (PGF). effect viability cells tested CCK-8 method, binding between PGF assessed with docking technology. mRNA protein expression RT-qPCR Western blotting.We identified 278 candidate 1081 KIRC-related targets, 7 involved therapeutic against KIRC. Among these showed significantly upregulated (P < 0.001) correlation a poor prognosis (HR=1.37, P=0.043). Molecular that energy -5.43 kcal/mol. assay at concentration 50 μmol/L capable inhibiting proliferation cells, higher resulted stronger inhibitory effect. results blotting treatment reduced both levels cells.Natural can suppress inhibit cells.