Iron excretion in iron dextran-overloaded mice.

• DOI: 10.2450/2014.0288-13
• Publication Date: 2014-10-01
• Authors: Marco Musumeci; Stefania Maccari; Alessia Raffaella...; Tonino Stati; Paola Sestili; Elisa Corritore; Augusto Alberto P...; Paolo Stacchini; Giuseppe Marano; Liviana Catalano
• Keywords: Excretory system

Iron homeostasis in humans is tightly regulated by mechanisms aimed to conserve iron for reutilisation, with a negligible role played excretory mechanisms. In previous study we found that mice have an astonishing ability tolerate very high doses of parenterally administered dextran. Whether this linked the existence pathway remains be ascertained.Iron overload was generated intraperitoneal injections dextran (1 g/kg) once week 8 weeks two different mouse strains (C57bl/6 and B6D2F1). Urinary faecal excretion assessed inductively coupling plasma-mass spectrometry, whereas cardiac liver architecture evaluated echocardiography histological methods. For both strains, 24-hour faeces urine samples were collected concentration determined on days 0, 1 2 after administration.In iron-overloaded C57bl/6 mice, increased 218% 157% 2, respectively (p<0.01). The excreted represented loss 14% total administered. Similar but smaller changes also B6D2F1 mice. Conversely, no significant either examination showed accumulation heart which tended decrease over time.This indicates mechanism removal excess body provides insights into possible excretion.