Quercetin Increases Doxorubicin-Induced Apoptosis Through Oxidative DNA Damage in KATO III Gastric Cancer Cells

• DOI: 10.32592/ircmj.2021.23.4.350
• Publication Date: 2021-05-02T09:33:15Z
• Authors: Mingxiang Chen; Chunxiao Duan; Jun Pan
• Keywords: Viability assay; Glutathione reductase; Terminal deoxynucleotidyl transferase

Background: Gastric cancer is the most common gastrointestinal malignancy with an increasing incidence rate worldwide. Finding novel curative and preventive approaches that could target tumor cells without affecting normal overcome drug resistance will be tremendously useful. Objectives: This study aimed to evaluate effects of quercetin (QUE) in combination doxorubicin (DOX) on apoptosis its underlying mechanisms KATO III gastric cell line. Methods: The Que DOX viability were measured using MTT assay. Western blot was used for measurement γH2AX protein expression. expression levels 8-Hydroxy-2'-deoxyguanosine evaluated by enzyme-linked immunosorbent DCFH-DA fluorescence dye detect formation reactive oxygen species (ROS). activities antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, reductase, S-transferase) also assessed. For evaluation apoptosis, terminal deoxynucleotidyl transferase dUTP nick end labeling assay used. Results: Based findings, QUE significantly increased cytotoxic DOX. Besides, considerably γH2AX. Upon treatment, ROS increased, enzyme markedly decreased. Moreover, treatment resulted potentiation doxorubicin-induced cells, compared treated either or Conclusion: Overall, co-administration enhances cytotoxicity, increases levels, induces oxidative DNA damage, decreases cellular defense, thereby might promise a therapeutic regimen promoting clinical efficacy patients cancer.