Overexpression of HIF-2a-Dependent NEAT1 Promotes the Progression of Non-Small Cell Lung Cancer through miR-1013p/SOX9/Wnt/β-Catenin Signal Pathway

• DOI: 10.33594/000000026
• Publication Date: 2019-03-07T15:51:10Z
• Authors: Xiangjun Kong; Yue Zhao; Xinmeng Li; Zhenxia Tao; Mingming Hou; Hui Ma
• Keywords: MTT assay; Tumor progression

The present study aimed to explore the function of NEAT1 on non-small cell lung cancer (NSCLC), as well its underlying mechanisms.Quantitative realtime PCR (qRT-PCR) was used measure expression in NSCLC tissues and cells. MTT assay transwell were performed detect proliferation, migration invasion. Potential target genes identified via luciferase reporter assay. Protein analysis through western blotting.The expressions significantly higher both cells than normal controls. High associated with TNM stage (P=0.000) metastasis (P=0.000). knockdown inhibited invasion Hypoxia induction mediated by HIF-2α promoted EMT expressions. Moreover, miR-101-3p a NEAT1. We also found that SOX9 miR-101-3p. Oncogenic progression miR-101-3p/SOX9/Wnt/β-catenin signaling pathway.NEAT1 up-regulation induced over-expression could promote under hypoxic condition. takes part axis.