[Simultaneous determination of 10 phthalate metabolites in urine by ultra performance liquid chromatography -tandem mass spectrometry].

• DOI: 10.3724/sp.j.1123.2024.04002
• Publication Date: 2025-06-01
• Authors: Xiaoying Zhao; Hui Kang; Yanyan Wang; Wenhui Li; Ming Chen; Changhong Ke; Feng Qin; Xixiong Kang

Phthalates （PAEs） are widely employed as plasticizers in plastic products that used industrial， agricultural， food， medical， and other fields. PAEs relatively weakly bonded to through non-covalent interactions. Consequently， can easily leak from the product into environment， which exposes public food intake， skin absorption personal care products， by inhaling air. Related studies have shown endocrine-disrupting substances long-term exposure may result diseases of nervous， reproductive， cardiovascular immune systems. In addition， excessive trigger inflammatory responses induce tumors. Therefore， establishing a highly sensitive assay for determining PAE levels human body following is an important objective. generally half-lives less than 24 h； they rapidly metabolized enzymatic hydrolysis after entering excreted urine. most focused on metabolites target compounds； hence， be assessed analyzing types these metabolites. Herein， we established method simultaneously ten phthalate （PAE） urine using ultra performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）. The were separated ACQUITY UPLC BEH Phenyl column （50 mm×2.1 mm， 1.7 μm）. Gradient elution was performed 0.1% formic acid aqueous solution acetonitrile mobile phases， at flow rate 0.5 mL/min， temperature 40 ℃， sample size 20 μL. Data acquired negative-ion electrospray ionization （ESI） multiple reaction monitoring （MRM） modes， quantified isotope internal standard method. found specific， with exhibiting good linearities their linear ranges， limits detection （LODs） quantification （LOQs） 0.03-0.3 0.1-1 ng/mL， respectively. Under four quality control （QC） levels， intra-day inter-day precisions all ≤8.3%， accuracy ranged ‒10.5% 7.3%. assess samples 60 volunteers， 1‒6 kinds detected each volunteer. This sensitive， accurate， simple， efficient， suitable large-scale biological