Effect of miRNA-23a silencing on ultraviolet B-induced chronic photodamage to fibroblasts

• DOI: 10.3760/cma.j.issn.0412-4030.2014.02.006
• Publication Date: 2014-02-15
• Authors: Jiaan Zhang; Bingrong Zhou; Yanyan Hu; Wei Wu; Huibin Yin; Qian Zhang; Xian-fei Guo
• Keywords: Antagomir

Objective To evaluate the effect of miRNA-23a silencing on ultraviolet B (UVB)-induced chronic photodamage to fibroblasts.Methods Fibroblasts from human foreskin were divided into four groups:blank control group receiving untreated,UVB UVB irradiation only,miRNA-23a transfected with a antagomir,UVB + transfection antagomir followed by irradiation.UVB was carried out once day for five consecutive days at dose 10 mJ/cm2.Three after last irradiation,SA-β-galactosidase staining performed detect senescent cells,flow cytometry analyze cell cycle,and real-time PCR and Western blot measure mRNA protein expressions p53,p16 p21,respectively.Results Both percentage β-galactosidase-positive cells proportion G1-phase significantly higher in than ((94.60 ± 2.58)％ vs.(48.18 3.70)％,(85.06 1.52)％ vs.(57.48 2.01)％,both P＜ 0.05).Significant differences observed p21 between (all P ＜ 0.05).Conclusions The may suppress UVB-induced photodamage,which is likely be associated inhibition senescence-associated downstream signaling molecules.

Key words: Ultraviolet rays; Fibroblasts; MicroRNAs; Cell aging