Effect of 5,6-dihydrocyclopenta-1, 2-dithiole-3-thione on Müller cells under the high glucose

• DOI: 10.3760/cma.j.issn.1005-1015.2017.03.016
• Publication Date: 2017-05-25
• Authors: Qi Huang; Min Tian; Qi Zhou
• Keywords: Viability assay; Group A

Objective To investigate the cellular viability and mitochondrial reactive oxygen species (ROS) production of Muller cells under high glucose condition, explore protection role 5,6-dihydrocyclopenta-1, 2-dithiole-3-thione (CPDT) on cells.

Methods Muller from Sprague Dawley rats were divided into 5 groups randomly, including 25 mmol/L normal group (group A) 65 B). High with 45, 60, 70 μmol/L CPDT cultured them 72 hour was set as C, D E. Water soluble tetrazolium salt (WST)-8 used to measure viability. Flow cytometry active apoptosis index. The expression nuclear factor erythroid 2-related 2 (Nrf2), hemeoxygenase-1 (HO-1), Bcl-2 Bax protein measured by Western blot.

Results Compared A, WST-8 showed that apparently decreased in B (t=39.59, P 0.05), but recovered E (t=-4.17, -7.52; P<0.05). Compared FCM ROS levels higher (t=-30.99, B, (t=27.68, Bax, Nrf2 HO-1 increased (t=-11.03, –63.17, –11.44; P<0.05), while bcl-2 (t=7.861, Nrf2, (t=15.11, 26.59, 6.27; (t=-6.53, P<0.05).

Conclusions Under glucose, may reduce cells. It inhibit through activating Nrf2/HO-1 pathway balancing level ROS.

Key words: Diabetic retinopathy/pathophysiology; Microglia; NF-E2-related 2; Heme oxygenase (decyclizing); bcl-2-Associated X Protein