A5100650223- Gout, Hyperuricemia, Uric Acid
- Natural product bioactivities and synthesis
- Pharmacological Effects of Natural Compounds
- Inflammasome and immune disorders
- Systemic Lupus Erythematosus Research
- Ginseng Biological Effects and Applications
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Phytochemistry and Biological Activities
- Veterinary medicine and infectious diseases
- NF-κB Signaling Pathways
- Ethics in Business and Education
- Reproductive Biology and Fertility
- Polysaccharides and Plant Cell Walls
- Plant-Microbe Interactions and Immunity
- Traditional Chinese Medicine Analysis
- Traditional Chinese Medicine Studies
- Immune Response and Inflammation
- Phytochemistry and Bioactive Compounds
- Reproductive System and Pregnancy
- Nuclear Receptors and Signaling
- Kruppel-like factors research
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer-related gene regulation
- Legume Nitrogen Fixing Symbiosis
- Peroxisome Proliferator-Activated Receptors
Heilongjiang University of Chinese Medicine
2013-2024
Hangzhou Hospital of Traditional Chinese Medicine
2023
Nanjing University
2023
Sun Yat-sen University
2018
Jiamusi University
2017
Shanghai University
2010-2015
Shanghai University of Traditional Chinese Medicine
2010
Rhizoma Dioscoreae Nipponicae (RDN) is a widely used traditional Chinese herb, which to treat arthroncus, arthrodynia and arthritis. As known us, inflammatory mechanisms have played an important role in the occurrence, course prognosis of gouty arthritis (GA). The aim this study was determine characteristic expressed proteins synovium GA rat synovial cell. model induced by monosodium urate (MSU) crystal. Tissue samples were assayed immunohistochemical method. effects RDN on Stromal...
Rhizoma Dioscoreae Nipponicae, from Discorea nipponica, is a widely used traditional Chinese herb. It to treat arthroncus, arthrodynia, and arthritis. Hyperuricemia an important foundation of gouty The current study was aimed at investigating whether the effects total saponins Nipponicae on hyperuricemia were due renal organic ion transporters in potassium oxonate-induced mice. Hyperplasia synovial cells prepared Wistar rats induced by IL-1β (1 × 10(4) µg/mL). MTT screen active components...
Abstract Background Dioscorea nipponica Makino is widely used in traditional Chinese medicine to treat gouty arthritis. Methods Sixty male Wistar rats were divided into six groups: the normal group, model colchicine group (COL) and three total saponin groups (RDN) (high dose [160 mg/kg], middle [80 mg/kg] low [40 mg/kg]). HE staining was detect histopathologic changes of synovial tissue joint. Immunohistochemical method protein expressions P-38, p-P38, JNK, p-JNK, ERK1/2, p-ERK1/2, MEK1/2,...
The objective was to study the mechanism of reducing level uric acid by rhizoma dioscoreae nipponese.A total 40 rats were divided into four groups: A normal group, hyperuricemia benzbromarone group (9 mg/kg) and saponins from nipponese (TDN) (40 mg/kg). Adenine (100 ethambutol (250 used induce hyperuricemic rats. Immunohistochemical Western blotting methods detect mRNA proteins expressions rat organic anion transporter1 (rOAT1), transporter3 (rOAT3) urate (rURAT1) in kidneys different...
Dioscorea nipponica Makino is one of the most common used traditional Chinese drugs which are to treat gouty arthritis (GA). Nuclear factor-κB (NF-κB) pathway plays an important role during this process. In present study, we investigated effects total saponins from D. (TDN) on NF-κB in interleukin-1β (IL-1β) induced fibroblast-like synoviocytes (FLS).FLS were divided into three groups: Normal group, model was given 10 μg/L IL-1β induce proliferation, and TDN group (10 +100 TDN). 1 h, 24 48...
Gouty arthritis (GA) is an inflammatory disorder that associated with elevated serum levels of uric acid. Total saponins from Dioscorea nipponica Makino (TSDN) are a natural component ameliorates inflammation while also decreasing acid levels. The aim the present study was to unravel mechanism TSDN in gouty rats regard regulation formation neutrophil extracellular traps (NETs) via PI3K/AKT/mTOR axis. A total 40 Wistar were divided into 4 groups: normal, model, and rapamycin groups....
Abstract Rhizoma Dioscoreae Makino (RDM) is effective in treating gouty arthritis (GA) and hyperuricacidemia, especially promoting uric acid excretion reducing the inflammatory reaction. Bioactive constituents RDM are mainly steroidal saponins such as dioscin, trillin, protodioscin protogracillin. However, mechanism of its anti‐GA action still unclear, owing to complex pathological physiological characteristics GA, integration with multiple components, targets pathways. Herein, a GA rat...
Abstract Background Rheumatoid arthritis (RA) is a kind of systemic autoimmune disease, and the joint inflammation cartilage destruction are major features. Some traditional Chinese medicine have been discovered to exhibit regulatory roles in treatment RA. Forsythiaside A (FA) as an active ingredient isolated from forsythia suspensa has participate into regulation some diseases through improving inflammation. However, effects FA on progression RA keep indistinct. Methods IL‐1β (10 ng/mL)...
With the development of quality life, morbidity hyperuricemia is increasing year by year. At same time, it appears that this disease attacks young people currently. As study pathogenesis advanced, a series uric acid transporters were found during process. Meanwhile, definition transporterome was proposed. They divided into three groups according to functions: reabsorption proteins, excretion proteins and skeleton proteins. moment, drugs for hyperuricmia mainly include composition inhibitors...
To explore the prevention and protection effect of Diosocorea nipponica (DNM)) on acute gouty arthritis (AGA) rats based liver metabonomics, find potential biomarkers related pathways. AGA model were induced by monosodium urate crystal suspension. UPLC-TOF-MS coupled with pattern recognition technique was employed to out metabolic Eleven common identified. Among intervention targets in normal given DNM, 4 up-regulated, other down regulated. 5 metabolites up-regulated MSU The abnormal...
Gouty arthritis (GA) is an inflammatory disease caused by the deposition of monosodium urate in synovial membrane and cartilage due to a high concentration uric acid blood. Dioscorea nipponica Makino widely used clinic treat GA. Total saponins are its main components showed anti-inflammatory effect on GA previous study. The mitogen-activated protein kinase (MAPK)-peroxisome proliferator-activated receptor γ (PPARγ) signaling pathway plays key role during onset GA; however, little known about...
Background: The deposition of MSU crystals within joints and surrounding soft tissues can potentially elicit the onset GA. While there have been investigations regarding inflammatory role microRNA in gout disease, research on impact PMN-Exo inflammation GA joint is scarce.Objective: present study aims to isolate extract for subsequent situ infusion testing. objective was investigate injury.Method: sequencing performed tissue screen DE-miRNAs predict their respective target gene...
Objective To investigate the cellular viability and mitochondrial reactive oxygen species (ROS) production of Muller cells under high glucose condition, explore protection role 5,6-dihydrocyclopenta-1, 2-dithiole-3-thione (CPDT) on cells. Methods Muller from Sprague Dawley rats were divided into 5 groups randomly, including 25 mmol/L normal group (group A) 65 B). High with 45, 60, 70 μmol/L CPDT cultured them 72 hour was set as C, D E. Water soluble tetrazolium salt (WST)-8 used to...
Plants initiate immunity by cell surface pattern-recognition receptors (PRRs) perceiving non-self molecules. PRRs are mostly receptor serine/threonine (Ser/Thr) kinases evolutionarily related to animal interleukin-1 receptor-associated kinase (IRAK)/Pelle-soluble kinases. However, how the activity of these is modulated remains poorly understood. Here, we report that Arabidopsis PRR CHITIN ELICITOR RECEPTER KINASE 1 (CERK1) undergoes autophosphorylation in unelicited cells at tyrosine428...
Neutrophil extracellular traps(NETs) are networks of fibers primarily composed DNA, histones, granular proteins, and cytoplasmic proteins released to the outside cells by neutrophils under stimulation bacteria, fungi, viruses, parasites, etc. NETs generated in two forms, suicidal vital NETs, according different stimuli. have both anti-inflammatory pro-inflammatory effects. On one hand, they can play anti-microbial role resist inflammation capturing, fixing, killing invading pathogens, which...