[Set up drug lymphocyte stimulation test (3H-TdR) and observe its application in drug-induced liver injury].

• DOI: 10.3760/cma.j.issn.1007-3418.2012.03.010
• Publication Date: 2012-03-01
• Authors: Gaoyan Chen; Cheng-wei Chen; Qingchun Fu; Xiaojin Wang; Liu-da Ni; Xiaohua Jiang; Feng Zhou; Li-qin Shi; Rongtao Lai; Jun Yang
• Keywords: Idiosyncrasy; Drug withdrawal

To set up the drug lymphocyte stimulation test (DLST), as a diagnosis means for DILI which was immunity idiosyncrasy, improve Diagnosis, level of DILI.For 59 patients who diagnosed DILI, we separated their PBMC, exploring to suspicious caused then use methods 3H-TdR test, according mixed degree clear PBMC count specific activated by drug.We also group, negative control and Positive at same time. Preliminary experiments including best dose PHA concentration drug. We 40 healthy group in our control, explore them on every two groups handled results t-test.The could be exactly reflect PBMC's proliferation nearly when they were or sensitive When drug, index (SI) Obviously higher than 1.8. Form this there 28 DILI's positive (47.46%), SI from 1.9 43.08, average 22.49, lower 1.8, significantly (5.78+/-0.75/1.16+/-0.25, P less 0.05). Our suggested DLST has Higher specificity (94.92%) sensitivity (47.46%).DLST significance idiosyncrasy's it's reflected these patients' Proliferation explored second