Effect of Shen fu injection on the expressions of ORP150, XBP1 and CHOP mRNAs in hypoxic-ischemic neonatal rats

• DOI: 10.3760/cma.j.issn.1673-4165.2014.09.006
• Publication Date: 2014-09-28
• Authors: Xiuli Cao; Yujie Wan; Jun Wang; Yan Xu; Yan Li
• Keywords: XBP1; Intraperitoneal injection

Objective To investigate the effect of Shenfu injection on expressions endoplasmic reticulum stress-related factors oxygen-regulated protein 150 (ORP150), X-box binding 1(XBP1), and C/EBP homologous (CHOP) mRNAs in cerebral cortical neurons after hypoxic-ischemic brain damage (HIBD) neonatal rats.

Methods The 7 day-newborn Sprague-Dawley rats were randomly divided into sham operation, normal saline treatment (Shenfu injection, 10ml/kg per day, peritoneal for 3 days) groups. They redivided 3h, 6h, 12h, 24h, 3d 7d subgroups at different time pointsafter modeling(n=8 each group). A HIBD model was induced. Reverse transcriptionpolymerase chain reaction used to detect ORP150, XBP1, CHOP rat cortex.

Results The XBP1 operation group very week. The significantly upregulated compared those 3h modeling (all P< 0.05); reached peak 6 respectively. Then they decreased mildly closed level day 7. 12, 24h lower than 0.05). mRNA expression positively correlated with (r=0.649, 0.05).

Conclusions HIBD induces stress response. may be involved delayed neuronal process rats. downregulates inhibits response.

Key words: Hypoxia-Ischemia, Brain; Regulatory Factor X Transcription Factors; Transcription CHOP; Oxygen-Regulated Proteins; Gene Expression; Endoplasmic Reticulum; Shen Fu Zhu She Ye; Rats