Hepcidin promotes proliferation and migration of breast cancer cells

• DOI: 10.3760/cma.j.issn.1674-1927.2019.02.008
• Publication Date: 2019-04-15
• Authors: Hailing Cui; Fang Li; Xiuying Zhou; Daoqiang Zhang
• Keywords: CXCL1

Objective To investigate the effect of Hepcidin on proliferation and migration breast cancer cell line MDA-MB-231 its mechanism.

Methods The serum samples 58 patients schedμled for operation, who were admitted to Wendeng Central Hospital in Weihai between April 2014 May 2016, healthy controls included study. The expression was determined by enzyme-linked immunosorbent assay (ELISA) . After adding at concentrations 0, 1, 2, 4 μmol/L cells, methylthiazoletetrazolium (MTT) used examine each group, examined scratch test. levels CXCL1 CXCR2 a concentration measured real-time quantitative PCR (qRT-PCR) Western blotting determine CXCR2, PI3K, AKT their phosphorylation levels.

Results The level hepcidin were[27.01 (19.80, 32.65) ]ng/ml [15.05 (10.49, 22.00) respectively, which showed significant difference two groups (U=769, P<0.01) promoted MDA-MB-231; (2.42±0.37 vs 0.89±0.11) , [mRNA: (2.91±0.17 1.13±0.11) protein (0.836±0.048 0.458±0.022) ], PI3K (0.920±0.053 0.561±0.067) (0.905±0.041 0.547±0.071) significantly higher than those (all .

Conclusion Hepcidin is highly expressed patients. promotes CXCL1/CXCR2, thereby activate PI3k/AKT signaling pathway, promote MDA-MB-231.

Key words: Hepcidin; Breast cancer; CXCL1/CXCR2; Proliferation; Migration