Effects of fluoxetine and pyrrolidine dithiocarbamate on the lipopolysaccharide-induced interleukin 6 release in cultured rat astrocytes

• DOI: 10.3760/cma.j.issn.1674-6554.2013.02.002
• Publication Date: 2013-02-20
• Authors: A. Dong; Wenwei Xu; Haihong Li; Lili Zhao; Yue Wu
• Keywords: Pyrrolidine dithiocarbamate

Objective To investigate the effects of fluoxetine and pyrrolidine dithidarbamate(PDTC) on lipopolysaccharide(LPS)-induced interleukine-6(IL-6) release in cultured rat astrocytes.

Methods The purified astrocytes were 48-well tissue culture plate classified into control group, LPS group PDTC group.Control as usual, with or at different concentrations for 48 hours, then Fluoxetine incubated 1ug/ml 24 hours. Finally, levels IL-6 cell supernatant detected by enzymatic linked immunosorbent assay (ELISA) method.

Results The level ((1975.46±171.54)pg/ml) was significantly higher than that group((10633±782.15)pg/ml)(P<0.01). The (6198.6±379.4)pg/ml, (4973.6±132.5)pg/ml (4747.9±473.9)pg/ml respectively when 10μΜ, 20μΜ 40μΜ, (4821.6±180.8)pg/ml, (4735.7±620.0)pg/ml (3525.9±240.0)pg/ml 100μΜ, 150μΜ 200μΜ. There significant difference between (P<0.05), well (P<0.05).

Conclusion LPS can induce from astrocytes, while some suppress LPS-induced release.

Key words: Fluoxetine; Pyrrolidine dithiocarbamate; Astrocyte; Interleukine-6