Variant Polymorphisms of GST and XRCC Genes and the Early Risk of Age Associated Disease in Kazakhstan

• DOI: 10.5281/zenodo.1057847
• Publication Date: 2012-11-22
• Authors: Zeinep A. Berkimb...; Almagul T. Mansha...; Elmira Khussainova; Leyla Djansugurova
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; gene polymorphism; xenobiotic detoxification.; risk factors; DNA reparation; 16. Peace & justice; Cardiovascular disease; 3. Good health

It is believed that DNA damaging toxic metabolites contributes to the development of different pathological conditions. To prevent harmful influence of toxic agents, cells developed number of protecting mechanisms, such as enzymatic reaction of detoxification of reactive metabolites and repair of DNA damage. The aim of the study was to examine the association between polymorphism of GSTT1/GSTM1 and XRCC1/3 genes and coronary artery disease (CAD) incidence. To examine a polymorphism of these genes in CAD susceptibility in patients and controls, PCR based genotyping assay was performed. For GST genes, frequency of GSTM1 null genotype among CAD affected group was significantly increased than in control group (P<0.001). Frequencies of the GSTT1 null and positive alleles are almost equal in both groups (P>0.1). We found that neither XRCC1 Arg399Gln nor XRCC3 Thr241Met were associated with CAD risk. 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