A5080891366- Bladder and Urothelial Cancer Treatments
- DNA Repair Mechanisms
- Multiple and Secondary Primary Cancers
- Genetic factors in colorectal cancer
- PARP inhibition in cancer therapy
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Acute Lymphoblastic Leukemia research
- Pediatric Hepatobiliary Diseases and Treatments
- Pancreatitis Pathology and Treatment
- Advanced Radiotherapy Techniques
- Gallbladder and Bile Duct Disorders
- Lung Cancer Treatments and Mutations
- Ovarian cancer diagnosis and treatment
- Cancer Genomics and Diagnostics
- Advances in Oncology and Radiotherapy
- Carcinogens and Genotoxicity Assessment
- Esophageal and GI Pathology
- Gastroesophageal reflux and treatments
- Liver Disease and Transplantation
- Eosinophilic Esophagitis
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Pancreatic function and diabetes
- Radiation Therapy and Dosimetry
University of Massachusetts Chan Medical School
2018-2024
Harvard University
2013-2016
Dana-Farber Cancer Institute
2013-2016
Pennsylvania State University
2013
Indiana University – Purdue University Indianapolis
1985-1990
Indiana University School of Medicine
1988-1990
University of Louisville
1990
University Medical Center
1990
University Hospital and Clinics
1990
Office of Science
1990
Cisplatin-based chemotherapy is the standard of care for patients with muscle-invasive urothelial carcinoma. Pathologic downstaging to pT0/pTis after neoadjuvant cisplatin-based associated improved survival, although molecular determinants cisplatin response are incompletely understood. We performed whole-exome sequencing on pretreatment tumor and germline DNA from 50 carcinoma who received followed by cystectomy (25 "responders," 25 pT2+ "nonresponders") identify somatic mutations that...
Platinum and PARP inhibitor (PARPi) sensitivity commonly coexist in epithelial ovarian cancer (EOC) due to the high prevalence of alterations homologous recombination (HR) DNA repair pathway that confer both drugs. In this report, we describe a unique subset EOC with another pathway, nucleotide excision (NER) which may exhibit discordance sensitivities these Specifically, 8% high-grade serous from The Cancer Genome Atlas dataset exhibited NER alterations, including nonsynonymous or splice...
The accuracy and clinical relevance of enteroclysis in the evaluation 138 patients referred for suspected Crohn disease small intestine are reported. original prospective interpretations results were assessed after a follow-up period 2 or more years. With all considered, had sensitivity, specificity, 100%, 98.3%, 99.3%, respectively, with only one false-positive diagnosis no false-negative diagnoses. Thirty-one percent (n = 43) lesions early disease. All who required surgery 23) advanced...
CRISPR (clustered regularly interspaced short palindromic repeats) genome editing holds promise in the treatment of genetic diseases that currently lack effective long-term therapies. Patients with alpha-1 antitrypsin (AAT) deficiency develop progressive lung disease due to loss AAT's antiprotease function and liver a toxic gain common mutant allele. However, it remains unknown whether CRISPR-mediated AAT correction liver, where is primarily expressed, can correct either or both defects....
Abstract Relapse in T-cell acute lymphoblastic leukemia (T-ALL) may signify the persistence of leukemia-initiating cells (L-ICs). Ectopic TAL1/LMO expression defines largest subset T-ALL, but its role leukemic transformation and impact on relapse-driving L-ICs remain poorly understood. In TAL1 / LMO mouse models, double negative-3 (DN3; CD4 − CD8 CD25 + CD44 ) thymic progenitors harbored L-ICs. However, only a DN3 exhibited L-IC activity, studies linking chemotolerance are needed. To...
Treatment options for patients with pancreatic ductal adenocarcinoma (PDAC) remain limited. Therapeutic targets of interest include mutated molecules that predispose to cancer such as KRAS and TP53. Here, we show an element the homologous recombination pathway DNA repair, PARP-binding protein C12orf48/PARI (PARPBP), is overexpressed specifically in cells where it appealing candidate targeted therapy. PARI upregulation or avian DT40 conferred repair deficiency genomic instability....
Synthetic glucocorticoids (GCs), such as dexamethasone and prednisone, remain key components of therapy for patients with lymphoid malignancies. For pediatric acute lymphoblastic leukemia (ALL), response to GCs remains the most reliable prognostic indicator; failure respond GC correlates poor event-free survival. To uncover resistance mechanisms, we performed a genome-wide, survival-based short hairpin RNA screen identified orphan nuclear receptor estrogen-related receptor-β (ESRRB) critical...
RNA sequencing (RNA-seq) has become a standard method for profiling gene expression, yet genomic DNA (gDNA) contamination carried over to the library poses significant challenge data integrity. Detecting and correcting this is vital accurate downstream analyses. Particularly, when samples are scarce invaluable, it becomes essential not only identify but also correct gDNA maximize data's utility. However, existing tools capable of limited lack thorough evaluation. To fill gap, we developed...
Upfront radiation therapy consisting of brachytherapy with or without external beam is considered standard care for patients endometrial carcinoma who are unable to undergo surgical intervention. This study evaluated the cancer-free survival (CFS), cancer-specific (CSS), and overall (OS) managed definitive-intent therapy.This was a single-institution retrospective analysis medically inoperable biopsy-proven up-front, definitive at UMass Memorial Medical Center between May 2010 October 2021....
A patient with the painless onset of a cholecystocolonic fistula associated virtually complete common bile duct obstruction due to stones provided unique opportunity assess consequences prolonged acid depletion on digestion and absorption nutrients. Over 2 years, insidiously developed steatorrhea, osteomalacia an atraumatic pelvic fracture, congestive heart failure complicated by polymorphic ventricular tachycardia (torsade de pointes) all which could be attributed malabsorption fat...
Should genetically engineered animals be patented? This issue has been one of the most contentious as lawmakers have grappled with how best to protect intellectual property. Since 1980 case Diamond v. Chakrabarty, in which U.S. Supreme Court ruled that a living microorganism is patentable, Patent and Trademark Office determined plants nonhuman can patented. These policy decisions led congressional debate on whether should patentable subject matter. Patenting organisms unique for three...