- Plant-Microbe Interactions and Immunity
- Entomopathogenic Microorganisms in Pest Control
- Genomics and Phylogenetic Studies
- Fungal Biology and Applications
- Insect Resistance and Genetics
- Plant Pathogens and Fungal Diseases
- Nematode management and characterization studies
- Nail Diseases and Treatments
- Microbial Metabolism and Applications
- Enzyme Production and Characterization
- Identification and Quantification in Food
- Plant Parasitism and Resistance
- Eosinophilic Disorders and Syndromes
- Insect symbiosis and bacterial influences
- Insect and Pesticide Research
- Microbial Community Ecology and Physiology
- Legume Nitrogen Fixing Symbiosis
- Plant Stress Responses and Tolerance
- Agricultural Economics and Practices
- Plant Pathogens and Resistance
- Plant tissue culture and regeneration
- Mycorrhizal Fungi and Plant Interactions
- Aluminum toxicity and tolerance in plants and animals
- Chromium effects and bioremediation
- Autoimmune Bullous Skin Diseases
University of California, San Francisco
2024
Karachi Medical and Dental College
2023
Liaquat National Hospital
2023
Jinnah Sindh Medical University
2023
Ziauddin University
2023
Dow University of Health Sciences
2023
Chandka Medical College
2023
Aga Khan University
2023
Lenox Hill Hospital
2023
Hayatabad Medical Complex
2021-2023
Nicotiana otophora is a wild parental species of tabacum, an interspecific hybrid N. tomentosiformis and sylvestris. However, least understood as alternative paternal donor. Here, we compared the fully assembled chloroplast (cp) genome with those closely related species. The analysis showed cp size 156,073 bp exhibited typical quadripartite structure, which contains pair inverted repeats separated by small large single copies, containing 163 representative genes, 165 microsatellites...
Abstract A liquid-crystal (LC)-filled transmission electron microscopy (TEM) grid cell coated with the cationic surfactant dodecyltrimethylammonium bromide (DTAB), to which a single-stranded deoxyribonucleic acid probe (ssDNA ) was adsorbed at LC/aqueous interface (TEM DTAB/DNA ), applied for highly specific detection of target DNA molecules. The DTAB-coated E7 (used LC mixture) in TEM DTAB exhibited homeotropic orientation, and changed planar orientation upon adsorption ssDNA . then exposed...
Serratia marcescens RSC-14 is a Gram-negative bacterium that was previously isolated from the surface-sterilized roots of Cd-hyperaccumulator Solanum nigrum. The strain stimulates plant growth and alleviates Cd stress in host plants. To investigate genetic basis for these traits, complete genome obtained by single-molecule real-time sequencing. S. comprised 5.12-Mbp-long circular chromosome containing 4,593 predicted protein-coding genes, 22 rRNA 88 tRNA 41 pseudogenes. It contained genes...
AGEs are a heterogeneous group of molecules formed from the nonenzymatic reaction reducing sugars with free amino groups proteins, lipids, and/or nucleic acids. have been shown to play role in various conditions including cardiovascular disease and diabetes. In this study, we hypothesized that “multiple hit hypothesis” nonalcoholic fatty liver (NAFLD) contribute pathogenesis hepatosteatosis. We measured effects mouse chows containing high or low AGE presence fat content on weight epididymal...
Oryza minuta (Poaceae family) is a tetraploid wild relative of cultivated rice with BBCC genome. O. has the potential to resist against various pathogenic diseases such as bacterial blight (BB), white backed planthopper (WBPH) and brown plant hopper (BPH). Here, we sequenced annotated complete mitochondrial genome minuta. The mtDNA 515,022 bp, containing 60 protein coding genes, 31 tRNA genes two rRNA genes. organization gene content at nucleotide level are highly similar (89%) that...
Current study aimed at exploring the diversity of bacterial endophytes with Boswellia sacra and their role in copper (Cu) stress to tomato plants. Bacterial were belonged Bacillus, Rhizobium Paenibacillus, which screened against Cu (0–10 mM) dwarf normal rice seeds. Among strains, Bacillus amyloliquefaciens BSL16 showed significantly higher bioremediation potential by accumulating high promoting growth B. increased plants during 2.5 mM stress. Active colonization reduced accumulation leaf,...
A bioassay-guided column chromatographic strategy was adopted in the present study to fractionate culture extract of Photorhabdus temperata M1021 identify potential insecticidal and antimicrobial compounds. An ethyl acetate (EtOAc) P. assayed against Galleria mellonella larvae through intra-hemocoel injection exhibited 100% insect mortality within 60 h. The EtOAc fraction an isolated compound phenoloxidase (PO) inhibition up 60% 63%, respectively. identified as 1,2-benzenedicarboxylic acid...
The present study demonstrates cloning, expression, and characterization of hyperthermostable L ‐asparaginase from Thermococcus kodakarensis KOD1 in Escherichia coli BLR(DE3). recombinant 6× His‐tagged protein T. (TkAsn), was purified to homogeneity by heat treatment followed affinity chromatography using a nickel‐nitrilotriacetic acid (Ni‐NTA) column. molecular mass the enzyme found be approximately 37 kDa sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE). enzymatic...
In the present study, different types of gibberellins (GAs) in culture filtrate (CF) Photorhabdus temperata M1021 were quantified. The analysis CF helped profiling various bioactive GAs: GA1, GA3, GA4, and GA7. Several physiologically inactive GA9, GA12, GA20 detected as well. Siderophore production was also investigated by growing P. on chrome azurol-S blue agar plates. Furthermore, strain inoculated into 'Waito-C' (Oryza sativa L.) rice plants, which significantly (P < 0.05) increased...
Boswellia sacra (Burseraceae), a keystone endemic species, is famous for the production of fragrant oleo-gum resin. However, genetic make-up especially genomic information about chloroplast still unknown. Here, we described first time (cp) genome B. sacra. The complete cp sequence revealed circular 160,543 bp size with 37.61% GC content. typical quadripartite structure inverted repeats (IRs 26,763 bp) separated by small single copy (SSC; 18,962 and large (LSC; 88,055 regions. De novo...
It is not known whether the prevalence of childhood esophagitis has changed over time. In children, intraepithelial eosinophil considered hallmark esophageal inflammation. This study compares eosinophils in biopsies obtained from 1980-1988 and 2001-2002.This reevaluated all at a single center during 2 periods: 2001-2002. Histological appearances were characterized per high-power field quantified.In total, 1058 510 subjects reviewed. Between 1980 1988, 247 188 evaluated. Of these, 62 met...