A5032426101- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Microtubule and mitosis dynamics
- Microfluidic and Bio-sensing Technologies
- Force Microscopy Techniques and Applications
- Lipid Membrane Structure and Behavior
- Genetic Neurodegenerative Diseases
- Fungal and yeast genetics research
- Skin and Cellular Biology Research
- Advanced Fluorescence Microscopy Techniques
- Micro and Nano Robotics
Delft University of Technology
2018-2023
Technical University of Munich
2018
Raman Research Institute
2017-2018
Max Planck Computing and Data Facility
2018
Bottom-up biology is an expanding research field that aims to understand the mechanisms underlying biological processes via in vitro assembly of their essential components synthetic cells. As encapsulation and controlled manipulation these elements a crucial step recreation such cell-like objects, microfluidics increasingly used for production minimal artificial containers as single-emulsion droplets, double-emulsion liposomes. Despite importance cell morphology on cellular dynamics, current...
Abstract Growing microtubule ends organize end-tracking proteins into comets of mixed composition. Here using a reconstituted fission yeast system consisting end-binding protein Mal3, kinesin Tea2 and cargo Tip1, we found that these can be driven liquid-phase droplets both in solution at under crowding conditions. In the absence agents, cryo-electron tomography revealed motor-dependent consist disordered networks where multivalent interactions may facilitate non-stoichiometric accumulation...
Although cytoskeletal networks are interpenetrating and interacting in living cells, very little is understood as to the effect their interaction has on properties. Here, a step towards elucidating synergistic cellular role of these structural proteins, we investigate isolated keratin actin composites show how vitro network formation influences properties vice versa. By encapsulating purified composite into vesicles separating time scales able demonstrate that stabilizes by providing an...
Changes in cell-substrate adhesion are believed to signal the onset of cancer metastasis, but such changes must be quantified against background levels intrinsic heterogeneity between cells. Variations strengths can probed through biophysical measurements cell detachment from substrates upon application an external force. Here, we investigate, theoretically and experimentally, cells adhered when these subjected fluid shear. We present a theoretical framework within which calculate fraction...
Abstract Growing microtubule ends provide platforms for the accumulation of plus-end tracking proteins that organize into comets mixed protein composition. Using a reconstituted fission yeast system consisting end-binding Mal3, kinesin Tea2 and cargo Tip1, we found these can be driven liquid phase droplets both in solution at under crowding conditions. In absence agents, cryo-electron tomography revealed motor-dependent consist disordered networks where multivalent interactions appear to...
Abstract Changes in cell-substrate adhesion are believed to signal the onset of cancer metastasis, but such changes must be quantified against background levels intrinsic heterogeneity between cells. Variations strengths can probed through biophysical measurements cell detachment from substrates upon application an external force. Here, we investigate, theoretically and experimentally, cells adhered when these subjected fluid shear. We present a theoretical framework within which calculate...
Abstract Regulation of adhesion is a ubiquitous feature living cells, observed during processes such as motility, antigen recognition or rigidity sensing. At the molecular scale, myriad mechanisms are necessary to recruit and activate essential proteins, while at cellular scale efficient regulation relies on cell’s ability adapt its global shape. To understand role shape remodeling adhesion, we use synthetic biology approach design minimal model, starting with limited number building blocks....