A5100320835- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- HER2/EGFR in Cancer Research
- Bacteriophages and microbial interactions
- Fire dynamics and safety research
- Computational Fluid Dynamics and Aerodynamics
- Peptidase Inhibition and Analysis
- Clostridium difficile and Clostridium perfringens research
- Insect Utilization and Effects
- Fluid Dynamics and Turbulent Flows
- Combustion and flame dynamics
- vaccines and immunoinformatics approaches
- Bacterial Identification and Susceptibility Testing
- Metabolomics and Mass Spectrometry Studies
- CAR-T cell therapy research
- Physiological and biochemical adaptations
- Chemical Synthesis and Analysis
- Melanoma and MAPK Pathways
- Carbohydrate Chemistry and Synthesis
- Combustion and Detonation Processes
- Protein Degradation and Inhibitors
- Fungal Plant Pathogen Control
- Cellular transport and secretion
- Advanced Numerical Methods in Computational Mathematics
- Transgenic Plants and Applications
University of Maryland, College Park
2018-2023
Park University
2021
Sericultural Research Institute
2019
Jiangsu University of Science and Technology
2019
Lysosome-targeting chimeras (LYTACs) offer an opportunity for the degradation of extracellular and membrane-associated proteins interest. Here, we report efficient chemoenzymatic method that enables a single-step site-specific conjugation high-affinity mannose-6-phosphate (M6P) glycan ligands to antibodies without need protein engineering conventional click reactions would introduce "unnatural" moieties, yielding homogeneous antibody-M6P conjugates targeted proteins. Using trastuzumab...
Site-specific labeling and conjugation of antibodies are highly desirable for fundamental research developing more efficient diagnostic therapeutic methods. We report here a general robust chemoenzymatic method that permits one-pot site-specific functionalization antibodies. A series selectively modified disaccharide oxazoline derivatives were designed, synthesized, evaluated as donor substrates different endoglycosidases antibody Fc glycan remodeling. found among several tested, wild-type...
Targeted degradation using cell-specific lysosome targeting receptors is emerging as a new therapeutic strategy for the elimination of disease-associated proteins. The liver-specific human asialoglycoprotein receptor (ASGPR) particularly attractive leveraged targeted protein (TPD). However, efficiency different glycan ligands ASGPR-mediated lysosomal delivery remains to be further characterized. In this study, we applied chemoenzymatic Fc remodeling method construct an array site-specific...
Monoclonal antibodies (mAbs) are one of the most rapidly growing drug classes used for treatment cancer, infectious and autoimmune diseases. Complement-dependent cytotoxicity (CDC) is effector functions to deplete target cells. We report here an efficient chemoenzymatic synthesis structurally well-defined conjugates a monoclonal antibody with rhamnose- αGal trisaccharide-cluster recruit natural anti-rhamnose anti-αGal antibodies, respectively, enhance CDC-dependent targeted cell killing. The...
Antibody-drug conjugates (ADCs) hold great promise for targeted cancer cell killing. Site-specific antibody-drug conjugation is highly desirable synthesizing homogeneous ADCs with optimal safety profiles and high efficacy. We have recently reported that azide-functionalized disaccharide oxazolines of the Manβ1,4GlcNAc core were an efficient substrate wild-type endoglycosidase Endo-S2 Fc glycan remodeling conjugation. In this paper, we report synthesis evaluation new as enzyme substrates...
A new Barely Implicit Correction (BIC) algorithm is presented for the simulation of low-Mach-number flows. This based on original, introduced by G. Patnaik et al. [G. Patnaik, R. H. Guirguis, J. P. Boris and E. S. Oran, barely implicit correction flux-corrected transport. In: Journal Computational Physics 71.1 (1987), pp. 120], which was a solution procedure including an explicit predictor step to solve convective portion Navier–Stokes equations corrector remove acoustic limit integration...
Glycosylation plays important roles in SARS-CoV-2 infection. We describe here a facile chemoenzymatic synthesis of core-fucosylated N-glycopeptides derived from the Spike protein and their binding with glycan-dependent neutralizing antibody S309 human lectin CLEC4G. The synthetic glycopeptides provide tools for further functional characterization viral glycosylation.
An efficient one-pot M6P glycan remodeling of a multiply glycosylated lysosomal enzyme (Lumizyme) affords new glycoengineered protein that shows greatly improved receptor binding, cellular uptake, and degradation glycogen in an vitro model Pompe disease.
Fluoride tolerance is an important economic trait in sericulture, especially some industrial development regions. Analyses of physiological changes involving structural damage to the insect body and molecular analyses related genes have focused on this area; however, that occur at metabolic level silkworms after eating fluoride-contaminated mulberry leaves remain unclear. Here, metabonomic analysis was conducted using gas chromatography-mass spectrometry (GC-MS) analyze midgut tissue NaF...
In this paper, the Barely Implicit Correction (BIC) algorithm is applied to simulation of low-Mach-number, reactive flows. The BIC a solution procedure consisting an explicit predictor step solve convective portion Navier-Stokes equations and implicit corrector which removes acoustic limit on integration time step. We show how include reaction processes into procedure. then four test problems with successively increased difficulty. First, series one-dimensional flames are computed, showing...
The incorporation of well-designed antibiotic nanocarriers, along with an adjuvant effect, in combination various antibiotics, offers opportunity to combat drug-resistant strains. However, precise control over morphology and encapsulated payload release can significantly impact their antibacterial efficacy synergistic effects when used alongside antibiotics. Here, this study focuses on developing lipopeptide-based nanoantibiotics, which demonstrate effect by inducing pH-induced collapse...
Fc-glycosite-specific antibody-drug conjugation represents a promising direction for the preparation of site-specific conjugates (ADCs). In present research, we conducted systemic evaluation two endoglycosidase-catalyzed chemoenzymatic glycoengineering technologies to prepare glycosite-specific ADCs. first two-step approach, antibody was deglycosylated and then reglycosylated with modified intact N-glycan oxazoline. second one-pot antibodies were simultaneously glycosylated functionalized...
A fire whirl forms when there is strong coupling of circulation and combustion reactive material. This work demonstrates the capability a recently developed numerical model to simulate whirl. We consider square enclosure that open at top closed bottom with air forced in along vertical corners apply circulation. constant flux heptane injected center wall. The solves compressible Navier-Stokes equations using barely implicit correction corrected transport algorithms. chemical energy release...