A5044322389- Food Allergy and Anaphylaxis Research
- T-cell and B-cell Immunology
- Parasitic Diseases Research and Treatment
- Allergic Rhinitis and Sensitization
- Insects and Parasite Interactions
- Glycosylation and Glycoproteins Research
- Insect symbiosis and bacterial influences
- Monoclonal and Polyclonal Antibodies Research
- Contact Dermatitis and Allergies
- Immune Cell Function and Interaction
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- Antifungal resistance and susceptibility
- Systemic Lupus Erythematosus Research
- Renal cell carcinoma treatment
- Mast cells and histamine
- Hematopoietic Stem Cell Transplantation
- Immune Response and Inflammation
- RNA and protein synthesis mechanisms
- Streptococcal Infections and Treatments
- Reproductive System and Pregnancy
- Biochemical and Structural Characterization
- Cancer Genomics and Diagnostics
- Asthma and respiratory diseases
- Eosinophilic Esophagitis
Fred Hutch Cancer Center
2023-2024
Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa
2024
Vanderbilt University Medical Center
2018-2024
Vanderbilt University
2020-2023
University of Tehran
2018
Kowsar Hospital
2018
Colony stimulating factor 1 (CSF1) and interleukin 34 (IL34) signal via the CSF1 receptor to regulate macrophage differentiation. Studies in IL34- or CSF1-deficient mice have revealed that IL34 function is limited central nervous system skin during development. However, roles of at homeostasis context inflammatory diseases cancer wild-type not been clarified vivo. By neutralizing and/or adult mice, we identified they play important differentiation, specifically steady state microglia,...
Single-cell spatial transcriptomics promises a highly detailed view of cell's transcriptional state and microenvironment, yet inaccurate cell segmentation can render this data murky by misattributing large numbers transcripts to nearby cells or conjuring nonexistent cells. We adopt methods from ab initio simulation rapidly infer morphologically plausible boundaries that preserve type heterogeneity. Benchmarking applied datasets generated three commercial platforms show superior performance...
Sensitization to Aspergillus species is associated with allergic respiratory diseases. Allergen immunotherapy nonstandardized extracts commonly used as therapy in these patients. Unfortunately, no method exists measure the relevant allergen protein content diagnostic and therapeutic extracts. Thus, there a critical need for extract standardization. We hypothesized that development of Aspergillus-specific human IgE mAbs would allow characterization allergenic epitopes among currently...
Inhibiting the kinase IRAK4 may suppress a multicellular autoinflammatory loop in patients with lupus.
Much of our understanding the targets IgE comes from studies allergy, though little is known about natural immunogenic seen after parasitic worm infections.We used human monoclonal antibodies (mAbs) for an unbiased and comprehensive characterization immunodominant antigens targeted by in conditions like allergy or helminth infection that are associated with elevated levels IgE.Using hybridoma technology to immortalize encoding B-cells peripheral blood subjects filarial infections IgE, we...
Background Tropical pulmonary eosinophilia (TPE) is a chronic respiratory syndrome associated with Lymphatic Filariasis (LF), tropical parasitic infection of the human, transmitted by mosquitoes. The larval form LF (microfilariae) are trapped in lungs TPE subjects have major role initiating syndrome. To date, there no reports on potential allergen that responsible for generating parasite-specific IgE TPE. Methodology/Principal findings In this project, we screened cDNA expression library...
Abstract Bacterial binding to host receptors underlies both commensalism and pathogenesis. Many streptococci adhere protein-attached carbohydrates expressed on cell surfaces using Siglec-like regions (SLBRs). The precise glycan repertoire recognized may dictate whether the organism is a strict commensal versus pathogen. However, it currently not clear what drives receptor selectivity. Here, we use five representative SLBRs identify of site that are hypervariable in sequence structure. We...
The Siglec-like Serine-Rich Repeat (SRR) adhesins mediate bacterial attachment to mammalian hosts via sialoglycan receptors. Here, we combine structural, computational, biochemical, and phylogenetic approaches elucidate the determinants of sialoglycan-binding spectrum across family SRR adhesins. We further identified mutable positions that disproportionately affect selectivity, as demonstrated by increases in binding alternative ligands 2- 3-orders magnitude. Biologically, these studies...
Human leukocyte antigen (HLA) gene is a highly polymorphic region. HLA typing required to match patients and donors for transplantation; therefore, development of registries necessary finding donors. system informative, numerous studies have been conducted on allele distribution in different populations.In this study, 100 unrelated Iranian individuals were typed HLA-A locus using sequence-based method. Samples subjected the PCR, followed by Sanger sequencing software analysis.A*02:01 (13%)...
Abstract Tropical Pulmonary Eosinophilia (TPE) is an asthma-like hyper-responsive syndrome characterized by accumulation of a high number eosinophils in the lungs leading to severe respiratory morbidity patients infected with lymphatic filariasis (LF), helminth parasitic infection that affects 51 million people 47 different countries worldwide. Subjects showing TPE also have circulating microfilariae (larval stages LF) and titer parasite-specific IgE their blood. are thought play major role...
<h3>Background</h3> Renal cell carcinoma (RCC) is a prevalent and aggressive form of kidney cancer, with variable response rates to checkpoint blockade immunotherapies. While high T infiltration often indicates favorable immunotherapies, this correlation inconsistent in RCC. To accurately identify RCC patients who will benefit from additional markers are needed differentiate between subsets tumor-infiltrating cells. Recent advances single-cell sequencing subcellular spatial transcriptomics...
Abstract Tropical pulmonary eosinophilia (TPE) is a chronic respiratory syndrome associated with Lymphatic Filariasis (LF), tropical parasitic infection of the human, transmitted by mosquitoes. A larval form LF, microfilariae trapped in lungs TPE subjects have major role initiating syndrome. To date, there are no reports on potential allergen that responsible for generating parasite-specific IgE TPE. In this project, we screened cDNA expression library microfilarial stages Wuchereria...
Abstract According to WHO estimates, more than 1.5 billion people (&gt;24% worldwide) are living with some type of helminth infection and there is no FDA-approved anti-helminth vaccine available. The 2 immune response understood be involved in our defense against infection. In developing countries, elevated serum IgE level a hallmark parasitic disease. However, many aspects the human still unknown; including, identity proteins targeted by antibodies size complexity functional repertoire....