A5085273044- Graphene and Nanomaterials Applications
- Carbon and Quantum Dots Applications
- Nanoparticles: synthesis and applications
- Graphene research and applications
- Nanoplatforms for cancer theranostics
- Advanced Sensor and Energy Harvesting Materials
- Hydrogels: synthesis, properties, applications
- Advanced Nanomaterials in Catalysis
- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Anesthesia and Neurotoxicity Research
- 2D Materials and Applications
- Nanocluster Synthesis and Applications
- Nanoparticle-Based Drug Delivery
- Polydiacetylene-based materials and applications
- Luminescence and Fluorescent Materials
- Advanced Materials and Mechanics
- Supercapacitor Materials and Fabrication
- Neuroscience and Neural Engineering
- Polymer composites and self-healing
- Microplastics and Plastic Pollution
- Electrochemical sensors and biosensors
- Enzyme-mediated dye degradation
- Dielectric materials and actuators
- Geophysical Methods and Applications
Universidad Carlos III de Madrid
2021-2024
Université de Strasbourg
2018-2023
Centre National de la Recherche Scientifique
2018-2023
Immunologie, Immunopathologie et Chimie Thérapeutique
2022
University of Castilla-La Mancha
2015-2020
University of Puerto Rico at Carolina
2019-2020
University of Trieste
2020
CIC biomaGUNE
2020
Hospital General Universitario de Ciudad Real
2018-2019
Bipar
2019
We present an overview of the main techniques for production and processing graphene related materials (GRMs), as well key characterization procedures. adopt a 'hands-on' approach, providing practical details procedures derived from literature authors' experience, in order to enable reader reproduce results.
The potential risks associated with two-dimensional (2D) nanomaterials may cause serious concerns about their real applications and impact in biological systems.
Abstract Impressive properties make graphene-based materials (GBMs) promising tools for nanoelectronics and biomedicine. However, safety concerns need to be cleared before mass production of GBMs starts. As skin, together with lungs, displays the highest exposure GBMs, it is fundamental importance understand what happens when get in contact skin cells. The present study was carried out on HaCaT keratinocytes, an vitro model toxicity, which effects four were evaluated: a few layer graphene,...
Biodegradability of graphene is one the fundamental parameters determining fate this material in vivo. Two types aqueous dispersible graphene, corresponding to single-layer (SLG) and few-layer (FLG), devoid either chemical functionalization or stabilizing surfactants, were subjected biodegradation by human myeloperoxidase (hMPO) mediated catalysis. Graphene was also studied presence activated, degranulating neutrophils. The degradation both FLG SLG sheets confirmed Raman spectroscopy...
Graphene based nanomaterials induce a reactive oxygen species-mediated mitochondrial depolarization, caused by the activation of NADH dehydrogenase and xanthine oxidase.
Abstract In tissue engineering strategies, the design of scaffolds based on nanostructures is a subject undergoing intense research: nanomaterials may affect properties, including their ability to interact with cells favouring cell growth and improving performance. Hydrogels are synthetic materials widely used obtain realistic constructs, as they resemble living tissues. Here, different hydrogels varying content graphene, synthesised by in situ radical polymerization acrylamide aqueous...
Graphene is an innovative nanomaterial, made of two-dimensional honeycomb-like carbon lattice, with potential in many different applications. Studying the behaviour graphene-related materials (GRMs) biological systems is, therefore, crucial to assess possible side effects. In this work studied biotransformation and impact few-layer pristine graphene (FLG) oxide (GOX), following ingestion as exposure route. To mimic FLG GOX ingestion, vitro digestion assay based on a standardized operating...
Abstract Carbon‐based nanomaterials have demonstrated to be potent candidates for biomedical applications. Recently, graphene quantum dots (GQDs) emerged as an attractive tool bioimaging, biosensing, and therapy. Hence, studying their biodegradability in living systems is essential speed up the translation toward real clinical innovations. Here, enzymatic degradation of GQDs using human myeloperoxidase eosinophil peroxidase investigated. Transmission electron microscopy, fluorescence, Raman...
The design of multifunctional materials able to both selectively deliver a drug into cells in targeted manner and display an enhanced propensity for biodegradation is important goal. Here, graphene oxide (GO) functionalized with the chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (fMLP) known interact formyl receptor, which expressed different cancer cells, including cervical carcinoma cells. This study highlights ability GOfMLP delivery cell killing subsequent degradation...
Polymer-based hydrogels, in particular those containing nanoscale fillers, are currently regarded as promising candidates for a plethora of applications. With respect to graphene, the vast majority publications concern chemical derivatives, and, consequence, knowledge potential pristine graphene within these systems is lacking. In this study, novel graphene-based hydrogels nonoxidized have been prepared by mild aqueous process. The mechanical and electrical properties hybrid materials were...
Fluorescence based on quantum confinement is a property restricted to the nanoscopic range. The incorporation of nanoparticles in three-dimensional polymeric network could afford macroscopic scaffolds that show properties. Moreover, if these are strong bonds, stability resulting materials can be preserved, thus enhancing their final applications. We report for first time preparation graphene dot (GQD) composite cationic covalent network. This new material has unusual features: (i) remains...
Besides inhalation, skin contact may be considered one of the most relevant exposure routes to graphene-based materials (GBMs). However, very few data on cutaneous toxicity these are available, so far. This study is focused irritation potential a panel GBMs: few-layer graphene (FLG), exfoliated by ball milling graphite, FLG ultrasonication using sodium dodecyl sulfate (FLG-SDS) or dodecylbenzenesulfonate (FLG-SDBS), CVD-graphene, obtained chemical vapor deposition, oxide (GO) and reduced GO...
Low doses of GO or FLG produce a metabolic remodeling that differentially affects human skin cell death and motility.
The use of graphene nanomaterials (GNMs) for biomedical applications targeted to the central nervous system is exponentially increasing, although precise information on their effects brain cells lacking. In this work, molecular changes induced in cortical astrocytes by few-layer (FLG) and oxide (GO) flakes are addressed. results show that exposure FLG/GO does not affect cell viability or proliferation. However, proteomic lipidomic analyses unveil alterations several cellular processes,...
Understanding the biodegradability of graphene materials by action oxidative enzymes secreted immune cells is essential for developing applicable biomedical products based on these materials. Herein, we demonstrate biodegradation oxide (GO) recombinant eosinophil peroxidase (EPO) enzyme extracted from human eosinophils in presence a low concentration hydrogen peroxide and NaBr. We compared degradation capability three different GO samples containing degrees oxygen functional groups their...
As an emerging member of the graphene family, structurally defined nanoribbons (GNRs) have shown promising applications in various fields. The evaluation degradability GNRs is particularly important for assessing persistence level and risk these materials living organisms environment. However, there a void study degradation GNRs. Here, we report behavior presence human myeloperoxidase (hMPO) or treated with photo-Fenton (PF) reaction. With assistance potassium hydroxide imidazole, which...
Graphene (G), graphene oxide (GO) and quantum dots (GQDs) have been introduced into a three-dimensional polymeric network based on polyacrylamide in order to ascertain the role of each nanomaterial hydrogels.
Skin provides the first interface between body and environment, representing one of most feasible exposure routes to graphene-based materials (GBMs). However, interactions GBMs with skin are poorly understood. In particular, low-concentration effects have not been investigated. Here we explored ability endotoxin-free, few-layer graphene (FLG) dehydrated oxide (d-GO) initiate an inflammatory response at cutaneous level by using human HaCaT keratinocytes. cell low concentrations (0.01–1.0...
Graphene-related materials (GRMs) are widely used in various applications due to their unique properties. A growing number of reports describe the impact different carbon nanomaterials, including graphene oxide (GO), reduced GO (rGO), and nanotubes (CNT), on immune cells, but there is still a very limited studies graphene. In this work, we investigated biological responses few layer (FLG) mouse macrophages (bone marrow derived macrophages, BMDMs), which part first line defense innate...
Wound infection is inevitable in most patients suffering from extensive burns or chronic ulcers, and there an urgent demand for the production of bactericidal dressings to be used as grafts restore skin functionalities. In this context, present study explores fabrication plasma-derived fibrin hydrogels containing hybrids based on graphene oxide (GO). The were fully characterized regarding gelation kinetics, mechanical properties, internal hydrogel structures by disruptive cryo scanning...
Graphene related materials (GRMs) are currently being used in products and devices of everyday life this strongly increases the possibility their ultimate release into environment as waste items. GRMs have several effects on plants, graphene oxide (GO) particular, can affect pollen germination tube growth due to its acidic properties. Despite socio-economic importance sexual reproduction seed effect process is still largely unknown. Here, Corylus avellana L. (common Hazel) was germinated...