A5008357873- Amyotrophic Lateral Sclerosis Research
- Neurogenetic and Muscular Disorders Research
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- biodegradable polymer synthesis and properties
- Heat shock proteins research
- Trace Elements in Health
- Crystallography and molecular interactions
- Exercise and Physiological Responses
- Computational Drug Discovery Methods
- Crystal structures of chemical compounds
- Mitochondrial Function and Pathology
- Molecular Sensors and Ion Detection
- ATP Synthase and ATPases Research
- Metal complexes synthesis and properties
- Synthetic Organic Chemistry Methods
- Lanthanide and Transition Metal Complexes
- Endoplasmic Reticulum Stress and Disease
- Biotin and Related Studies
- Muscle metabolism and nutrition
- RNA Research and Splicing
- Aluminum toxicity and tolerance in plants and animals
- Click Chemistry and Applications
- Neurological diseases and metabolism
- Muscle Physiology and Disorders
The University of Melbourne
2011-2020
Florey Institute of Neuroscience and Mental Health
2013-2015
Parks Victoria
2014
Mental Health Research Institute
2012
Radiolabeled diacetylbis(4-methylthiosemicarbazonato)copper II [Cu (atsm)] is an effective positron-emission tomography imaging agent for myocardial ischemia, hypoxic tumors, and brain disorders with regionalized oxidative stress, such as mitochondrial myopathy, encephalopathy, lactic acidosis stroke-like episodes (MELAS) Parkinson’s disease. An excessively elevated reductive state common to these conditions has been proposed important mechanism affecting cellular retention of Cu from...
Abnormal processing of TAR DNA binding protein 43 (TDP-43) has been identified as a major factor in neuronal degeneration during amyotrophic lateral sclerosis (ALS) or frontotemporal lobar (FTLD). It is unclear how changes to TDP-43, including nuclear cytosolic translocation and subsequent accumulation, are controlled these diseases. TDP-43 member the heterogeneous ribonucleoprotein (hnRNP) RNA family known associate with stress granule proteins ALS FTLD. hnRNP trafficking accumulation by...
Amyotrophic lateral sclerosis (ALS) is a progressive, fatal, motor neuron disease with no effective long-term treatment options. Recently, TDP-43 has been identified as key protein in the pathogenesis of some cases ALS. Although role degeneration not yet known, shown to accumulate RNA stress granules (SGs) cell models and spinal cord tissue from ALS patients. The SG association may be an early pathological change metabolism such potential target for therapeutic intervention. Accumulation SGs...
Cytosolic accumulation of TAR DNA binding protein 43 (TDP-43) is a major neuropathological feature amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). However, the mechanisms involved in TDP-43 remain largely unknown. Previously, we reported that inhibitors cyclin-dependent kinases (CDKs) prevented cytosolic stress granule TDP-43, correlating with depletion heterogeneous ribonucleoprotein (hnRNP) K from granules. In present study, further investigated...
XFM approach detects subcellular zinc and calcium mishandling in a fatal neurodegenerative disease, that is corrected by delivery of bioavailable zinc.
The intracellular distribution of fluorescently labeled copper and zinc bis(thiosemicarbazonato) complexes was investigated in M17 neuroblastoma cells primary cortical neurons with a view to providing insights into the neuroprotective activity complex known as CuII(atsm). Time-resolved fluorescence measurements allowed identification CuII ZnII well free ligand inside by virtue distinct lifetime each species. Confocal fluorescent microscopy treated copper(II)bis(thiosemicarbazonato) revealed...
Abstract To take advantage of the luminescent properties d 6 transition metal complexes to label proteins, versatile bifunctional ligands were prepared. Ligands that contain a 1,2,3‐triazole heterocycle synthesised using Cu I catalysed azide–alkyne cycloaddition “click” chemistry and used form phosphorescent Ir III Ru II complexes. Their emission readily tuned, by changing either ion or co‐ligands. The tethered metalloprotein transferrin several conjugation strategies. /Ru –protein...
Skeletal myogenesis is a coordinated sequence of events associated with dramatic changes in cell morphology, motility, and metabolism, which causes cellular stress alters proteostasis. Chaperones, such as heat-shock proteins (HSPs), play important roles limiting stresses maintaining proteostasis, but whether HSPs are specifically involved not well understood. Here, we characterized gene protein expression subcellular localization various proliferating C2C12 myoblasts differentiating myotubes...
In response to injury, skeletal muscle stem cells (MuSCs) undergo myogenesis where they become activated, proliferate rapidly, differentiate and fusion form multinucleated myotubes. Dramatic changes in cell size, shape, metabolism motility occur during which cause cellular stress alter proteostasis. The molecular chaperone heat shock protein 70 (HSP70) maintains proteostasis by regulating biosynthesis folding, facilitating transport of polypeptides across intracellular membranes preventing...
Background TAR-DNA binding protein 43 (TDP-43) is a heterogeneous ribonucleoprotein (hnRNP) identified as major constituent of cytosolic inclusions in spinal cord patients with motor neuron disease. The are formed by movement TDP-43 from its predominantly nuclear localization to the cytosol, followed accumulation ubiquitinated and phosphorylated C-terminal fragments. mechanisms controlling trafficking not well known, however, it has been demonstrated previously that kinases control hnRNPs....