A5013947507- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Mycology
- Helicobacter pylori-related gastroenterology studies
- Bacteriophages and microbial interactions
- Legionella and Acanthamoeba research
- Bacterial biofilms and quorum sensing
National Jewish Health
2021-2024
University of Colorado Denver
2021-2024
Rationale: Healthcare-associated transmission of nontuberculous mycobacteria (NTM) among people with cystic fibrosis (pwCF) has been investigated at CF centers worldwide, conflicting conclusions. We the Colorado Adult Program. Objectives: To systematically investigate healthcare-associated and/or acquisition NTM to determine similarity respiratory and environmental isolates, compare home residence watershed mapping pwCF having genetically similar isolates. Methods: Whole-genome sequencing...
Rationale: Outbreaks of nontuberculous mycobacteria (NTM) among people with cystic fibrosis (pwCF) have been reported at CF centers conflicting conclusions. The occurrence NTM the UVMC (University Vermont Medical Center) adult program was investigated. Objectives: Use HALT (Healthcare-associated Links in Transmission NTM) toolkit to investigate healthcare-associated transmission and/or acquisition pwCF having genetically similar isolates. Methods: Whole genome sequencing isolates from 23...
Background Healthcare-associated transmission of nontuberculous mycobacteria (NTM) among people with cystic fibrosis (pwCF) has been reported and is increasing concern. No standardized epidemiologic investigation tool published for healthcare-associated NTM outbreak investigations. This report describes the design an ongoing observational study to standardize approach pwCF. Methods a parallel multi-site pwCF within single Center who have respiratory isolates identified as being...
Rationale Nontuberculous mycobacteria (NTM) has been reported to be transmitted between people with cystic fibrosis (CF) attending CF centres. A suspected Mycobacterium abscessus outbreak was investigated at the University of Texas Southwestern (UTSW) Adult Program using a combination pathogen genomic sequencing and epidemiologic methods. The objectives present study were apply Healthcare-Associated Links in Transmission NTM (HALT NTM) investigate occurrence potential healthcare-associated...
Mycobacterium abscessus subsp. (MABS) is an emerging, opportunistic pathogen found globally in freshwater biofilms and soil. Typically, isolates are treated as a uniform group of organisms very little known about their comparative survival healthy host cells. We posit that environmentally- clinically derived isolates, show differential infectivity immune cells resistance to innate defenses.Six MABS were tested including three water biofilm/soil sputum-derived isolates. A clinical type strain...
Free-living amoebae are ubiquitous in aquatic environments and act as environmental reservoirs for nontuberculous mycobacteria. Mycobacterium avium subsp. hominissuis recovered from Acanthamoeba has been demonstrated to be more virulent both human murine models. Here, we investigate the persistence of M. after short-term (2 weeks) long-term (42 co-culture lenticulata We hypothesize that A. lenticulata-adapted demonstrate phenotypic genomic changes facilitating intracellular naïve...