A5023247141- Wound Healing and Treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Mesenchymal stem cell research
- Neurogenesis and neuroplasticity mechanisms
- Adipose Tissue and Metabolism
- Hair Growth and Disorders
- Immune cells in cancer
- Cerebrospinal fluid and hydrocephalus
- Pressure Ulcer Prevention and Management
- Tendon Structure and Treatment
- Dermatology and Skin Diseases
- Immune Cell Function and Interaction
- Neuroinflammation and Neurodegeneration Mechanisms
- Body Contouring and Surgery
- Histone Deacetylase Inhibitors Research
- Fetal and Pediatric Neurological Disorders
- Immune Response and Inflammation
- Muscle Physiology and Disorders
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- CAR-T cell therapy research
- Fatty Acid Research and Health
- Mitochondrial Function and Pathology
- Low-power high-performance VLSI design
- Fibroblast Growth Factor Research
- Protist diversity and phylogeny
George Washington University
2021-2024
Yale University
2014-2020
Intel (United States)
2020
University of Connecticut
2008-2015
Myofibroblast diversity with injury and aging Fibroblasts deposit extracellular matrix (ECM) molecules to regulate tissue strength function. However, if too much ECM is deposited, fibrosis scarring results. Shook et al. examined cells during mouse skin wound healing, fibrosis, (see the Perspective by Willenborg Eming). They identified distinct subpopulations of myofibroblasts, including as adipocyte precursors (APs). In cellular ablation models, CD301b-expressing macrophages selectively...
The study of adipose tissue in vivo has been significantly advanced through the use genetic mouse models. While aP2-Cre(BI) and aP2-Cre(Salk) lines have widely used to target tissue, specificity these for adipocytes recently questioned. Here we characterize Cre recombinase activity multiple cell populations major depots other using membrane-Tomato/membrane-GFP (mT/mG) dual fluorescent reporter. We find that lack within line does not efficiently white depots. Alternatively, Adiponectin-CreERT...
Through adulthood, the rodent subventricular zone (SVZ) stem cell niche generates new olfactory bulb interneurons. We had previously reported that number of neurons produced in SVZ declines through aging; however, age-related changes attributable specifically to neural (NSC) population have not been fully characterized. Here, we conducted a spatiotemporal evaluation adult NSCs. assessed ventricle-contacting NSCs, which together with ependymal cells form regenerative units (pinwheels) along...
Adipocytes undergo pronounced changes in size and behavior to support diverse tissue functions, but the mechanisms that control these are not well understood. Mammary gland-associated white adipose (mgWAT) regresses of milk fat production during lactation expands subsequent involution milk-producing epithelial cells, providing one most marked physiological examples growth. We examined cellular functional implications adipocyte lipid dynamics mouse mammary gland (MG). Using vivo analysis...
The subventricular zone (SVZ) of the adult mouse brain is a narrow stem cell niche that lies along length lateral wall ventricles. SVZ supports neurogenesis throughout adulthood; however, with increasing age, ventral deteriorates and only dorsolateral remains neurogenic. Associated elderly SVZ, we reported previously an increased number astrocytes interposed within adjacent ependymal lining. Here, show integrated ependyma are dividing, BrdU-labeled share cellular adherens neighboring cells....
Summary Age‐associated ventriculomegaly is typically attributed to neurodegeneration; however, additional factors might initiate or contribute progressive ventricular expansion. By directly linking postmortem human MRI sequences with histological features of periventricular tissue, we show that substantial lateral ventricle surface gliosis associated ventriculomegaly. To examine whether loss ependymal cell coverage resulting in glial scarring can lead enlargement independent any other injury...
A novel machine learning based parasitic estimation (MLParest) method for pre-layout custom circuit design is presented. It reduces the error between and post-layout simulation from 37% to 8% on average different measurements across a variety of analog circuits. MLParest can thus greatly reduce number iterations phases. The key contributions this work are approach push-button model training framework, scalable technology nodes. To best our knowledge, framework an industry first.
Lipids have emerged as potent regulators of immune cell function. In the skin, adipocyte lipolysis increases local pool free fatty acids and is essential for coordinating early macrophage inflammation following injury. Here, we investigate G-protein-coupled receptor 84 (GPR84), a medium-chain acid (MCFA) receptor, its potential to propagate pro-inflammatory signaling after skin GPR84 was identified key component regulating myeloid numbers subsequent tissue repair through in vivo...
The ventricular system carries and circulates cerebral spinal fluid (CSF) facilitates clearance of solutes toxins from the brain. functional units ventricles are ciliated epithelial cells termed ependymal cells, which line through ciliary action capable generating laminar flow CSF at ventricle surface. This monolayer also provides barrier filtration functions that promote exchange between brain interstitial fluids (ISF) circulating CSF. Biochemical changes in thereby reflected composition...
Diabetic wound healing is associated with impaired function and reduced numbers of myofibroblasts, a heterogeneous cell population varying capacities to promote repair. To determine how diabetes alters myofibroblast composition, we performed flow cytometry spatial tissue analysis subsets throughout the process in diabetic (db/db) control (db/+) mouse skin. We observed profibrotic SCA1+; CD34+; CD26+ myofibroblasts wounds five days after injury, decreased expression fibrosis-associated genes...
The ventricular system carries and circulates cerebral spinal fluid (CSF) facilitates clearance of solutes toxins from the brain. functional units ventricles are ciliated epithelial cells termed ependymal cells, which line through ciliary action capable generating laminar flow CSF at ventricle surface. This monolayer also provides barrier filtration functions that promote exchange between brain interstitial fluids (ISF) circulating CSF. Biochemical changes in thereby reflected composition...
Abstract Tumor-associated macrophages (TAMs) play a critical role in shaping the tumor microenvironment (TME). Responding to numerous cues from various cell types present tumor, TAMs often exhibit spectrum of phenotypes, ranging between M1 (inflammatory) and M2 (tumor-supportive) macrophages. Therefore, M1/M2 macrophage ratio determines suppression or growth. Higher infiltration is usually associated with poor prognosis several cancer due their predisposition towards function. reprogramming...
Abstract The interplay between tumor cells and immune within the microenvironment (TME) dictates protumor or antitumor responses. Tumor-associated macrophages (TAMs) respond to signals of TME exhibit a spectrum phenotypes ranging M1 (antitumor) M2 (protumor) macrophages. In most types, including melanoma, balance is critical with higher M1/M2 ratio favoring immunity. Therefore, strategies enhancing can significantly alter towards this study, we administered luciferase GFP-expressing as an...