ABSTRACT Considering that little is known about the epidemiology of Neospora caninum infection in humans, particularly populations with high Toxoplasma gondii rates, present study aimed to investigate presence antibodies N. T. -seropositive and -seronegative individuals. A total 256 serum samples divided into four groups (61 from human immunodeficiency virus [HIV]-positive patients, 50 patients neurological disorders, 91 newborns, 54 healthy subjects) were assessed for serologies by indirect...

The role of other non-perfringens Clostridium species in endophthalmitis infections has not been analyzed. We describe a case and review prior cases the literature. Fulminant clinical presentations poor visual outcomes are rule, but combined aggressive medical surgical approach may conserve vision.

The present study was conducted to evaluate the frequency of antigenic components recognized by serum IgG antibodies in Western blotting (WB) using a Strongyloides ratti larval extract for diagnosis human strongyloidiasis. In addition, WB results were compared enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence antibody test (IFAT) results. Serum samples 180 individuals analyzed (80 with strongyloidiasis, 60 other intestinal parasitoses, 40 healthy individuals). S....

Several studies have shown that chronic alcoholics increased susceptibility to infections due higher exposure infectious agents as well breakdown in their immune defenses. As Strongyloides stercoralis infection is usually more relevant immunocompromised patients, the aim of this study was evaluate frequency S. alcoholics. Thus, coproparasitological examination carried out 145 subjects, from which 45 were (mean age 45.7 +/- 11.0 years), 10 nonalcoholic cirrhotic patients 50.2 13.1 and 90...

The present study was conducted to detected IgG antibodies using Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by enzyme-linked immunosorbent assay (ELISA). Sera from 90 subjects were analyzed (30 with strongyloidiasis, 30 other parasites and healthy individuals). Results expressed in antibody titers, which considered as positive when titer > 80. Sensibility specificity 100% 96.7%, respectively. It can be concluded that heterologous could employed...

To evaluate the rate of infection by Strongyloides stercoralis and other enteroparasites a survey was conducted in city Uberlândia, State Minas Gerais, Brazil. A total 900 stool samples from 300 children aging four months to seven years, randomly selected ten nursery schools September 1994 December 1995, were examined, both Baermann-Moraes Lutz methods. Thirty nine (13%) found be infected S. stercoralis, 64.1% boys 35.9% girls. Taking all as whole results pointed out that 265 (88.4%)...

The aim of this study was to determine the occurrence intestinal parasites and commensals among children in four peripheral districts located northern, southern, eastern western sectors Uberlândia, Minas Gerais, using Baermann methods as modified by Moraes Lutz. Out 160 individuals studied, 93 (58.1% CI: 50.4-65.7) were infected, distributed follows: northern (72.5%), southern (47.5%), (57.5%) (55%). positive findings according age groups were: 0-5 years (26.9%), 5-10 (21.2%) 10-15 (10%)....

IgG avidity assays have been developed for several parasitic diseases although there are no researches focused in strongyloidiasis diagnosis. Definitive diagnosis of is based on the presence Strongyloides larvae stool, but majority cases involve low and irregular larval output. While limitations serological well known, characteristics persons who misdiagnosed negative coproparasitological tests little explored. The aim present study was to evaluate use detect patients with active...

Cryo-microtome sections of larvae Strongyloides stercoralis and S. ratti respectively obtained from human rat feces cultures, were used as antigens. Fluoresceinate conjugates against IgG employed at the ideal titer 10 for 100 ratti. The sensitivity indirect immunofluorescence reaction (IIF) was 94.4% 92.5% specificity 94.2% 97.1% two specific larval antigens, respectively. Sera 123 persons (54 carriers infections 69 controls) submitted to reaction. titers different sera varied 20 2560. There...

This study examined the frequency of Strongyloides stercoralis infection in patients with gastrointestinal cancer through parasitological and immunological tests. A total 77 were evaluated, 33 44 controls other types cancers. All undergoing chemotherapy 14 (18.2%) receiving concomitant radiotherapy. For a diagnosis, we applied Baermann Lutz methods. The diagnosis involved indirect fluorescence antibody test (IFAT) enzyme linked immunosorbent assay (ELISA) to detect IgG antibodies using ratti...

Glycosylated components from Strongyloides have an important role in parasite establishment and host recognition of these substances. Considering the sugar-binding capacity lectins, such as concanavalin-A (Con-A), IgG IgA detection serum samples strongyloidiasis patients was tested using different antigenic preparations. The total saline extract (SE) venezuelensis filariform larvae fractionated Con-A column to obtain unbound (Con-A UF) bound BF) fractions. Sensitivity (Se), specificity (Sp),...

Background Strongyloidiasis, a human intestinal infection caused by the nematode Strongyloides stercoralis, is frequently underdiagnosed and although its high prevalence still neglected parasitic disease because conventional diagnostic tests based on parasitological examination (presence of larvae in stool) are not sufficiently sensitive due to low load irregular larval output. There an urgent need improve assays, especially for immunocompromised patients with as consequence self-infection...

Strongyloidiasis is a human parasitic disease caused by the helminth Strongyloides stercoralis whose treatment particularly difficult in immunosuppressed patients due to their low responsiveness conventional therapy. Carica papaya and its isolated compounds benzyl isothiocyanate, carpaine carpasemine are promising compound for of infections anthelmintic action. This study aims examine vitro ovicidal larvicidal activity C. seed hexane extract against venezuelensis, using egg hatching tests...

The aim of this study was to detect levels IgG and IgA by enzyme-linked immunosorbent assay (ELISA) using alkaline extracts larvae, adult female worms, eggs Strongyloides venezuelensis as antigen. One hundred twenty serum samples divided into 3 groups were analysed: group I (40 strongyloidiasis patients), II patients with other parasitic infections), III healthy subjects). Statistical variations analyzed analysis variance. There a significant statistical difference (P < 0.001) in the...