A5070110411- Mast cells and histamine
- Asthma and respiratory diseases
- Single-cell and spatial transcriptomics
- IL-33, ST2, and ILC Pathways
- Food Allergy and Anaphylaxis Research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Acute Myeloid Leukemia Research
- Hematopoietic Stem Cell Transplantation
- Spaceflight effects on biology
- Genetics, Aging, and Longevity in Model Organisms
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Eosinophilic Esophagitis
- 3D Printing in Biomedical Research
- Eosinophilic Disorders and Syndromes
Karolinska Institutet
2021-2023
Karolinska University Hospital
2021-2023
National University of Singapore
2015
Mast cell accumulation is a hallmark of number diseases, including allergic asthma and systemic mastocytosis. Immunoglobulin E-mediated crosslinking the FcεRI receptors causes mast activation contributes to disease pathogenesis. The lineage one least studied among hematopoietic lineages, controversies remain about whether expression appears during progenitor stage or terminal maturation. Here, we used single-cell transcriptomics analysis reveal temporal association between appearance gene...
Background Immunohistochemical analysis of granule-associated proteases has revealed that human lung mast cells constitute a heterogeneous population cells, with distinct subpopulations identified. However, systematic and comprehensive cell-surface markers to study cell heterogeneity yet be performed. Methods Human were obtained from lobectomies, the expression 332 was analyzed using flow cytometry LEGENDScreen™ kit. Markers exhibited high variance selected for additional analyses reveal...
Background: Basophils are implicated in various diseases including allergies, but a comprehensive characterization of human basophils has yet to be performed at the single-cell level. Objective: We aimed generate omics-based resource circulating basophils, made accessible research community. also sought investigate basophil heterogeneity transcriptional and surface epitope levels. Methods: Circulating were analyzed using short- long-read RNA-sequencing combination with large-scale...
Abstract In disease states with chronic inflammation, there is a crosstalk between mast cells and neutrophil granulocytes in the inflamed microenvironment, which may be potentiated by tryptase. systemic mastocytosis (SM), are constitutively active tryptase elevated blood. Mast cell activation SM leads to symptoms from various organs depending on where reside, for example, palpitations, flush, allergic including anaphylactic reactions, osteoporosis. Whether function altered not well...
Innate lymphoid cells (ILCs) play important roles in tissue homeostasis and host defense, but the proliferative properties migratory behavior of especially human ILCs remain poorly understood. Here we mapped at single-cell resolution spatial distribution quiescent within vascular versus compartment. For this purpose, employed MISTRG humanized mice as an in-vivo model to study ILCs. We uncovered subset-specific differences status between non-lymphoid organs. also identified CD117 - CRTH2...
<p dir="ltr">Mast cells play a critical role in the human immune system and adults originate from bone marrow through hematopoiesis. Mast are known to contribute allergic diseases, deregulation of mast cell development leads disorders such as mastocytosis. This thesis focused on investigating health mastocytosis using single-cell sequencing platform.</p><p dir="ltr">In Study I, we characterized circulating progenitors transcriptomics. We revealed presence previously...
<p dir="ltr">Mast cells play a critical role in the human immune system and adults originate from bone marrow through hematopoiesis. Mast are known to contribute allergic diseases, deregulation of mast cell development leads disorders such as mastocytosis. This thesis focused on investigating health mastocytosis using single-cell sequencing platform.</p><p dir="ltr">In Study I, we characterized circulating progenitors transcriptomics. We revealed presence previously...
Abstract Background Immunohistochemical analysis of granule-associated proteases have revealed that human lungs mast cells constitute a heterogeneous population cells, with distinct subpopulations identified. However, systematic and comprehensive cell surface markers to study lung heterogeneity is yet be performed. Methods Human were obtained from lobectomies the expression 332 analyzed using flow cytometry LEGENDScreen ™ kit. Markers exhibited high variance selected for additional analyses...
Abstract Mast cell accumulation is a hallmark of number diseases including allergic asthma and systemic mastocytosis. IgE-mediated crosslinking the FcεRI receptors causes mast activation contributes to disease pathogenesis. The lineage one least studied among hematopoietic lineages there are still controversies about identity progenitor, i.e., whether expression appears during progenitor stage or in maturing cells. Here, we used single-cell transcriptomics reveal temporal association between...