A5114705996- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Sports Performance and Training
- Literature: history, themes, analysis
- Cell Adhesion Molecules Research
- Sport Psychology and Performance
- Reformation and Early Modern Christianity
- Motor Control and Adaptation
- Immunotherapy and Immune Responses
- American Constitutional Law and Politics
- Galectins and Cancer Biology
- Physical Education and Pedagogy
- Immune Response and Inflammation
- Protein purification and stability
- Immunodeficiency and Autoimmune Disorders
- Toxin Mechanisms and Immunotoxins
- Erythrocyte Function and Pathophysiology
- Sports and Physical Education Research
- Sports injuries and prevention
- Lipid Membrane Structure and Behavior
- Cancer Genomics and Diagnostics
- Motivation and Self-Concept in Sports
- Natural product bioactivities and synthesis
Loughborough University
2024-2025
University of Oxford
1989-2024
University of Surrey
2024
University of America
2023
Catholic University of America
2023
Louisiana State University Health Sciences Center New Orleans
2022
Florida Institute for Human and Machine Cognition
2021
Franciscan University of Steubenville
2020
King's College London
2019
Brunel University of London
2009-2018
Abstract A fraction of la‐like glycoproteins was prepared from rat thymocytes by lentil lectin affinity chromatography and gel filtration in deoxycholate. Spleen cells mice immunized with this preparation were fused myeloma to produce antibody‐secreting hybrid cell lines. Antibody four lines called MRC OX, 3, 4, 5, 6 reacted the glycoproteins, OX 3 antibody recognized an antigenie determinant polymorphic rat. All antibodies also bound mouse spleen all detected polymorphisms. Studies on...
Abstract A new monoclonal mouse antibody that recognizes a subset of rat peripheral T cells has been prepared by immunizing mice with thymocyte glycoprotein. This antibody, designated MRC OX 8, labels all are unlabeled the previously described W3/25 antibody. No were found bound both antibodies, but, in contrast, 90% thymocytes doubly labeled. Thoracic duct lymphocytes congenitally athymic nude rats not labeled either but spleens such animals contained + and 8 cells. These splenocyte...
Abstract A rat Ia (RT‐1B) antigen (called Ia‐A) equivalent to the mouse I‐A product has been defined with monoclonal antibodies (W. R. McMaster and A. F. Williams, Eur. J. Immunol. 1979. 9 : 426). To identify other antigens were raised against spleen glycoproteins depleted of Ia‐A antigen. An IgG antibody MRC OX 17) was obtained used purify a molecule which had similar structure reacted anti‐Ia alloantibodies. There no cross‐reaction between two in assays antibodies, alloantibodies or rabbit...
A leukocyte-common (L-C) antigen which can be dominant as an immunogen in rabbit anti-rat thoracic duct lymphocyte serum has been purified from rat thymocytes. Initially, antigenic fragment of 100,000 apparent mol. wt. was prepared at 400 to 900-fold purification by lentil lectin affinity chromatography and gel filtration deoxycholate. Mice were then immunized with this fraction, a hybrid myeloma cell line secreting antibody the L-C fusion. This used for gave pure 1400-fold compared The is...
Cell surface molecules of eukaryotic cells have been considered to be integrated into the membrane bilayer by a transmembrane protein sequence. The Thy-1 antigen rodent thymocytes and brain was first molecule for which biochemical data clearly suggested integration via nonprotein tail. Direct evidence is now presented showing that glycophospholipid structure attached carboxyl-terminal cysteine residue 31 amino acids predicted from complementary DNA sequence are not present in mature...
Abstract The molecular forms and antigenic heterogeneity of the leukocyte‐common antigen (L‐CA) rat lymphocytes have been analyzed. Thymocytes show one main band at 180 kDa, T cells four bands 180,190, 200 220 kDa B broadband about 240 kDa. helper cytotoxic cell subsets same with some differences in proportion each. Four mouse monoclonal antibodies (MRC OX‐1, 28, 29 30) reacted all L‐CA fell into two sets that were noncompetitive binding to vs. OX‐30). determinants seen by these lost when...
The T-cell antigen receptor (TCR) complex is the key structure involved in signal transduction T cells. To analyze associations between TCR and other molecules, immunoprecipitations were carried out, followed by phosphorylation of molecules vitro tyrosine kinases associated with precipitated molecules. This provided a sensitive assay for molecular complexes, demonstrated surface antigens CD2, CD4, or CD8 CD5 normal rat complexes readily seen immunoprecipitates from Brij 96 but not Nonidet...
Abstract Anti‐rat immunoglobulin antibodies were purified by acid elution from immunoadsorbents, and their binding to rat thoracic duct lymphocytes thymocytes studied. Satisfactory was only achieved when pepsindegraded reagents used anomalous results obtained if aggregated antibody present. From the of PVG/c strain rat, 40–50 % cells bound 20000–150000 molecules anti‐rat Fab antibody, or 8000–60000 anti‐IgM reagent. Only 2.5 more than 8000 anti‐IgG 2a+2b few thymus any at this level....
Abstract Quantitative assays for measuring the binding of xenogeneic antiserum to dispersed cell suspensions are described. Cells were incubated with unlabeled and antibody bound measured indirectly by a second step 125 I‐labeled anti‐immunoglobulin antibody. This indirect radioactive assay was calibrated measuring, radioimmunoassay, true amount in first step. With these methods one can measure strength antisera, quantitate number antigenic sites, partially differentiate determinants being...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSelf-assembly of double and triple helices controlled by metal ion stereochemical preferenceClaude Piguet, Gerald Bernardinelli, Bernard Bocquet, Anna Quattropani, Alan F. WilliamsCite this: J. Am. Chem. Soc. 1992, 114, 19, 7440–7451Publication Date (Print):September 1, 1992Publication History Published online1 May 2002Published inissue 1 September...
<h3>Objective</h3> The epidemiology of violence against children is likely to differ substantially by sex and age the victim perpetrator. Thus far, investment in effective prevention strategies has been hindered lack clarity burden childhood across these dimensions. We produced first age-specific sex-specific prevalence estimates perpetrator type for physical, sexual emotional globally. <h3>Design</h3> used random effects meta-regression estimate prevalence. Estimates were adjusted relevant...
The Thy-1-molecule, which was identified by its antigenic activities, has been purified from rat thymocytes. purification involved preparation of crude membranes and solubilization in deoxycholate, followed gel filtration affinity chromatography on antibody or lectin columns. In all cases the molecule a glycoprotein that did not form higher polymers associated with other polypeptide chains. Thy-1 could be found two forms, one binding to lentil lectin, not. Both forms had same detectable...