A5053930908- Trypanosoma species research and implications
- Lysosomal Storage Disorders Research
- Insect symbiosis and bacterial influences
- Research on Leishmaniasis Studies
- Parasitic Infections and Diagnostics
- Studies on Chitinases and Chitosanases
- Cardiomyopathy and Myosin Studies
- Toxoplasma gondii Research Studies
- Mosquito-borne diseases and control
- Cytomegalovirus and herpesvirus research
- Biochemical and Molecular Research
- Coccidia and coccidiosis research
- Calcium signaling and nucleotide metabolism
Universidade Federal do Rio de Janeiro
2014-2024
National Institute of Science and Technology for Structural Biology and Bioimaging
2014-2023
Centro de Pesquisas Oncológicas
2023
Universidade do Estado do Rio de Janeiro
2006
The cytostome-cytopharinx complex is the main site of endocytosis Trypanosoma cruzi epimastigotes. Little known about detailed morphology this remarkable structure. We used serial electron tomography and focused ion beam-scanning microscopy to reconstruct entire complex, including surrounding cytoskeleton vesicles. Focusing on cells that had taken up gold-labeled tracers, we produced 3D snapshots process endocytosis. cytostome was composed two microtubule sets: a triplet started underneath...
Trypanosoma cruzi epimastigotes uptake nutrients by endocytosis via the cytostome-cytopharynx complex - an anterior opening (cytostome) continuous with a funnel-shaped invagination (cytopharynx) that extends to posterior of cell, accompanied microtubules. During metacyclogenesis transformation into human-infective metacyclic trypomastigotes disappears, as lose endocytic ability. To date, no studies have examined disappearance in detail, or determined if activity persists during...
The cytostome-cytopharynx complex is the main site for endocytosis in epimastigotes of Trypanosoma cruzi. It consists an opening at plasma membrane surface – cytostome - followed by a invagination cytopharynx. In G1-S cells, this structure associated with two specific sets microtubules quartet and triplet. Here, we used electron microscopy tomography to build 3D models different stages cell cycle. absent late G2 M phase while early cells have either short cytopharynx or no visible complex,...
Trypanosoma cruzi epimastigotes store high amounts of cholesterol and cholesteryl esters in reservosomes. These unique organelles are responsible for cellular digestion by providing substrates homeostasis parasite differentiation. Here we demonstrate that under nutritional lipid stress, preferentially mobilized reservosome stocks, instead bodies, leading to the consumption reservoirs production ergosterol. Starved acquired more LDL-NBD-cholesterol endocytosis distributed exogenous their...
SUMMARY Trypanosoma cruzi epimastigote reservosomes store nutrients taken up during the intense endocytic activity exhibited by this developmental form. Reservosomes were classified as pre-lysosomal compartments. In contrast, trypomastigote forms are not able to take from medium. Interestingly, trypomastigotes also have acidic organelles with same proteases contained in reservosomes. Nevertheless, origin and function of these been disclosed so far. Given similarities between compartments...
Trypanosomatidae, which includes eukaryotic species agents of diseases like leishmaniasis, sleeping sickness, and Chagas disease, have special structures organelles not found in mammalian cells. They present a layer microtubules, known as subpellicular microtubules (SPMT), located underneath the plasma membrane responsible for preserving cell morphology, polarity, position single copy organelles, morphological changes that occur throughout protozoan life cycle. Even though lot knowledge...
The highly adaptable parasite Trypanosoma cruzi undergoes complex developmental stages to exploit host organisms effectively. Each stage involves the expression of specific proteins and precise intracellular structural organization. These morphological changes depend on key structures that control components’ growth redistribution. In trypanosomatids, flagellar attachment zone (FAZ) connects flagellum cell body plays a pivotal role in expansion rearrangement. While FAZ are well-studied other...
The highly adaptable parasite Trypanosoma cruzi undergoes complex developmental stages to exploit host organisms effectively. Each stage involves the expression of specific proteins and precise intracellular structural organization. These morphological changes depend on key structures that control components' growth redistribution. In trypanosomatids, flagellar attachment zone (FAZ) connects flagellum cell body plays a pivotal role in expansion rearrangement. While FAZ are well-studied other...