A5002718646- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- Signaling Pathways in Disease
- Reproductive Physiology in Livestock
- Reproductive Biology and Fertility
- Biochemical and Structural Characterization
- Wnt/β-catenin signaling in development and cancer
- Growth Hormone and Insulin-like Growth Factors
- Stress and Burnout Research
- Hippo pathway signaling and YAP/TAZ
- Anesthesia and Pain Management
- Angiogenesis and VEGF in Cancer
- Protein Kinase Regulation and GTPase Signaling
- Health and Lifestyle Studies
- Pain Management and Opioid Use
- Birth, Development, and Health
- Alcohol Consumption and Health Effects
- Axon Guidance and Neuronal Signaling
- Antifungal resistance and susceptibility
- Microtubule and mitosis dynamics
- Ovarian function and disorders
- Folate and B Vitamins Research
- Cerebrovascular and Carotid Artery Diseases
- Nerve Injury and Rehabilitation
- Fungal Infections and Studies
Hospital Doctor Hernán Henríquez Aravena
2022-2024
Texas A&M University
2013-2023
Clínica Las Condes
2015-2023
Hospital Luis Calvo Mackenna
2023
Pontificia Universidad Católica de Valparaíso
2017-2021
Pontificia Universidad Católica de Chile
2016
Montreal Children's Hospital
2015
University of Chile
2001-2014
Instituto de Neurociencia Biomédica
2014
Mitchell Institute
2013
Regulation of actin dynamics through the Nck/N-WASp (neural Wiskott–Aldrich syndrome protein)/Arp2/3 pathway is essential for organogenesis, cell invasiveness, and pathogen infection. Although many proteins involved in this are known, detailed mechanism by which it functions remains undetermined. To examine signaling mechanism, we used a two-pronged strategy involving computational modeling quantitative experimentation. We developed predictions Nck-dependent polymerization using Virtual Cell...
Angiogenesis in the developing central nervous system (CNS) is regulated by neuroepithelial cells, although genes and pathways that couple these cells to blood vessels remain largely uncharacterized. Here, we have used biochemical, cell biological molecular genetic approaches demonstrate β8 integrin Neuropilin-1 (Nrp1) cooperatively promote CNS angiogenesis mediating adhesion signaling events between vascular endothelial cells. neuroepithelium promotes activation of extracellular matrix...
A critical transition in ovarian follicular development is the selection of a dominant follicle, capable ovulating, from cohort synchronously growing antral follicles. However, little known about mechanisms and factors that regulate growth We have investigated whether component insulin-like factor (IGF) system, namely IGFBP-4 protease, associated with establishment dominance cattle. IGFBP proteases degrade IGFBPs, freeing IGFs to interact their receptors. In experiment 1, fluid preovulatory...
The Nck family of Src homology (SH) 2/SH3 domain adaptors functions to link tyrosine phosphorylation induced by extracellular signals with downstream regulators actin dynamics. We investigated the role mammalian in signaling from activated platelet-derived growth factor (PDGF) receptor (PDGFbetaR) cytoskeleton. report here that are required for cytoskeletal reorganization and chemotaxis stimulated PDGF-B. Analysis tyrosine-phosphorylated proteins demonstrated Crk-associated substrate...
Cryptococcus neoformans (Cn) is a deadly fungal pathogen whose intracellular lifestyle important for virulence. Host mechanisms controlling phagocytosis and replication remain obscure. Here, we perform global phosphoproteomic analysis of the host response to infection. Our reveals numerous diverse proteins that are differentially phosphorylated following ingestion by macrophages, thereby indicating reprogramming kinase signaling. Notably, activates autophagy initiation complex (AIC) upstream...
Development of a dominant follicle is associated with decreased intrafollicular low molecular weight IGF-binding proteins (namely IGFBP-2, -4, and -5) increased proteolysis IGFBP-4 by pregnancy-associated plasma protein A (PAPP-A). In addition to proteolytic activity, bovine follicular fluid contains strong activity for IGFBP-5, but not IGFBP-2. Here we show that the IGFBP-5 protease present in neutral/basic pH-favoring, Zn2+ metalloprotease very similar previously described protease. We...
Dominant follicles are characterized by low levels of molecular weight IGF binding proteins (IGFBPs) and proteolytic activity against IGFBP-4 -5. To examine the hypothesis that proteolysis -5 plays a critical role in selection dominant follicle, we isolated at various stages during first wave follicular development bovine estrous cycle, using ultrasonography to follow growth. Ovariectomies were performed before divergence size (group 1; largest approximately 7 mm diameter), about expected...
Low molecular weight insulin-like growth factor binding proteins (IGFBPs), particularly IGFBP-4, are believed to inhibit the actions of factors (IGFs). We showed previously that ovarian follicular dominance in cattle is associated with presence a protease degrades IGFBP-4. To test hypothesis specific IGFBP-4 proteolysis selection dominant follicle, we induced codominant follicles (co-DFs) during first wave estrous cycle. The ovaries Holstein heifers were examined twice daily by...
The dynamic reorganization of actin structures helps to mediate the interaction cells with their environment. Abl non-receptor tyrosine kinase can modulate rearrangement during cell attachment. Here we report that PxxP motifs, which bind Src homology 3 (SH3) domains, are indispensable for coordinated regulation filopodium and focal adhesion formation cell-spreading dynamics Candidate PxxP-motif-binding partners were identified by screening a comprehensive SH3-domain phage-display library. A...
Directional migration requires the coordination of cytoskeletal changes essential for cell polarization and adhesion turnover. Extracellular signals that alter tyrosine phosphorylation drive directional by inducing reorganization actin cytoskeleton. It is recognized Nck an important link between dynamics, however, role in remodeling during underlying molecular mechanisms remain largely undetermined. In this study, a combination genetics quantitative live microscopy was used to show...
n-3 PUFA (polyunsaturated fatty acids), i.e. DHA (docosahexaenoic acid), found in fish oil, exhibit anti-inflammatory properties; however, the molecular mechanisms remain unclear. Since PtdIns(4,5)P2 resides raft domains and can alter size of rafts, we hypothesized that downstream actin remodelling are perturbed by incorporation into membranes, resulting suppressed T-cell activation. CD4+ T-cells isolated from Fat-1 transgenic mice (membranes enriched PUFA) exhibited a 50% decrease...
Directional cell motility is essential for normal development and physiology, although how motile cells spatiotemporally activate signaling events remains largely unknown. Here, we have characterized an adhesion unit comprised of protein tyrosine phosphatase (PTP)-PEST the extracellular matrix (ECM) receptor β8 integrin that plays roles in directional motility. PTP-PEST form complexes at leading edge migrating balance patterns Rac1 Cdc42 by controlling subcellular localization...