A5071466593- Plant Gene Expression Analysis
- Lignin and Wood Chemistry
- Biofuel production and bioconversion
- Plant biochemistry and biosynthesis
- Plant Molecular Biology Research
- Enzyme-mediated dye degradation
- Horticultural and Viticultural Research
- Biochemical and biochemical processes
- Bioinformatics and Genomic Networks
- Genomics and Phylogenetic Studies
- Gene expression and cancer classification
- Plant Stress Responses and Tolerance
- Natural Products and Biological Research
- Microbial Metabolic Engineering and Bioproduction
North Carolina State University
2013-2021
SCG Chemicals (Thailand)
2016
Laccases, as early 1959, were proposed to catalyze the oxidative polymerization of monolignols. Genetic evidence in support this hypothesis has been elusive due functional redundancy laccase genes. An Arabidopsis double mutant demonstrated involvement laccases lignin biosynthesis. We previously identified a subset genes be targets microRNA (miRNA) ptr-miR397a Populus trichocarpa . To elucidate roles and its targets, we characterized gene family 49 models, which 29 predicted ptr-miR397a....
A multi-omics quantitative integrative analysis of lignin biosynthesis can advance the strategic engineering wood for timber, pulp, and biofuels. Lignin is polymerized from three monomers (monolignols) produced by a grid-like pathway. The pathway in formation Populus trichocarpa has at least 21 genes, encoding enzymes that mediate 37 reactions on 24 metabolites, leading to affecting properties. We perturb these genes integrate transcriptomic, proteomic, fluxomic phenomic data 221 lines...
Plants develop tolerance to drought by activating genes with altered levels of epigenetic modifications. Specific transcription factors are involved in this activation, but the molecular connections within regulatory system unclear. Here, we analyzed genome-wide acetylated lysine residue 9 histone H3 (H3K9ac) enrichment and examined its association transcriptomes Populus trichocarpa under stress. We revealed that abscisic acid-Responsive Element (ABRE) motifs promoters drought-responsive...
We established a predictive kinetic metabolic-flux model for the 21 enzymes and 24 metabolites of monolignol biosynthetic pathway using Populus trichocarpa secondary differentiating xylem. To establish this model, comprehensive study was performed to obtain reaction inhibition parameters all based on functional recombinant proteins. A total 104 Michaelis-Menten 85 were derived from these enzymes. Through mass spectrometry, we obtained absolute quantities in This extensive experimental data...
Wood is an essential renewable raw material for industrial products and energy. However, knowledge of the genetic regulation wood formation limited. We developed a genome-wide high-throughput system discovery validation specific transcription factor (TF)–directed hierarchical gene regulatory networks (hGRNs) in formation. This depends on new robust procedure isolation transfection Populus trichocarpa stem differentiating xylem protoplasts. overexpressed Secondary Wall-Associated NAC Domain...
Significance Wood is a widely used renewable feedstock for industrial production and energy generation. The secondary cell wall (SCW) the major component of wood. Two key transcription factor families, Vascular-Related NAC-Domain (VND) Secondary Wall-Associated NAC Domain (SND), are master gene regulators SCW biosynthesis. However, plants exhibit stunted growth or abnormal development under excess VND SND expression. In this study, we show that two splice variants, PtrVND6-C1 IR PtrSND1-A2 ,...
Lignin, an abundant renewable aromatic biopolymer, is essential macromolecule in terrestrial vascular plants. Genetic modifications affecting monolignol biosynthesis, which produces lignin monomers, alter the metabolic flux through pathway and are becoming increasingly explored for improving biomass quality producing high-value commodity chemical feedstocks. Benzoate (BA) conjugates important metabolites plants that have not been adequately characterized as components of structure. In...
Co-enzyme A (CoA) ligation of hydroxycinnamic acids by 4-coumaric acid:CoA ligase (4CL) is a critical step in the biosynthesis monolignols. Perturbation 4CL activity significantly impacts lignin content diverse plant species. In Populus trichocarpa , two well-studied xylem-specific Ptr4CLs (Ptr4CL3 and Ptr4CL5) catalyze CoA acid to 4-coumaroyl-CoA caffeic caffeoyl-CoA. Subsequently, 4-hydroxycinnamoyl-CoA:shikimic hydroxycinnamoyl transferases (PtrHCT1 PtrHCT6) mediate conversion Here, we...