A5101729651- Colorectal Cancer Surgical Treatments
- Gastric Cancer Management and Outcomes
- Colorectal Cancer Treatments and Studies
- Genetic factors in colorectal cancer
- Inflammatory Biomarkers in Disease Prognosis
- Esophageal and GI Pathology
- Colorectal Cancer Screening and Detection
- Anorectal Disease Treatments and Outcomes
- Additive Manufacturing and 3D Printing Technologies
- Cancer Immunotherapy and Biomarkers
- Ferroptosis and cancer prognosis
- Hepatocellular Carcinoma Treatment and Prognosis
- Colorectal and Anal Carcinomas
- Cancer, Lipids, and Metabolism
- Metastasis and carcinoma case studies
- Cardiac, Anesthesia and Surgical Outcomes
- Fluid Dynamics and Heat Transfer
- Electrohydrodynamics and Fluid Dynamics
- Gastrointestinal disorders and treatments
- Gastrointestinal Tumor Research and Treatment
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer Cells and Metastasis
- Mycobacterium research and diagnosis
- Gut microbiota and health
- Pulmonary Hypertension Research and Treatments
Union Hospital
2015-2025
Huazhong University of Science and Technology
2015-2025
Union Hospital
2020-2025
Shenzhen Second People's Hospital
2024
Guangzhou University
2024
Shenzhen University
2024
Wuhan Union Hospital
2021
Tongji University
2021
Ischemia-reperfusion injury (IRI) is a major obstacle in liver transplantation, especially with steatotic donor livers. Dysbiosis of the gut microbiota has been implicated modulating IRI, and Lachnospiraceae plays pivotal role regulating host inflammatory immune responses, but its specific transplantation IRI remains unclear. This study explores whether can mitigate underlying mechanisms. We found Lachnospiraceae-bacterium (Lachn.) abundance was significantly reduced rats cirrhosis....
There is increasing need to expand availability of donor liver grafts, including steatotic livers. However, the current use grafts in transplantation less acceptable due their higher susceptibility ischemia-reperfusion (I/R) injury. To investigate mechanism underlying I/R injury, we detected cell death markers and inflammation clinical livers animal models. We found that caspase-8-mediated hepatic apoptosis activated I/R. ablation caspase-8 only slightly mitigated injury without affecting...
Abstract Obstructions can occur during any stage of colorectal cancer (CRC) and correspond with poor prognosis. Obstructive (OCRC) is harder exhibits increased tumor budding proliferation myofibroblasts compared to non-obstructive CRC, suggesting that the occurrence obstruction may be related extracellular matrix (ECM) remodeling. Here, we found CRC OCRC samples differed substantially in ECM composition, specifically collagen (newly formed mature) proteoglycans (including glycosaminoglycan,...
The tumor microenvironment (TME) is an important factor that regulates the progression of colorectal cancer (CRC). Cancer-associated fibroblasts (CAFs) are main mesenchymal cells in TME and play a vital role progression; however, specific underlying mechanisms require further study.Multiple single-cell transcriptome data were analyzed validated. Primary CAFs isolation, CCK8 assay, co-culture western blotting, multiple immunofluorescence, qRT-PCR, ELISA, immunoprecipitation, ChIP, double...
Abstract Background Tumour perineural invasion (PNI) is a predictor of poor prognosis, but its effect on the prognosis patients with colorectal cancer (CRC) has not yet been elucidated. Methods This retrospective study used propensity score matching (PSM). The clinical case data 1470 surgically treated stage I–IV CRC at Wuhan Union Hospital were collected. PSM was to analyse and compare clinicopathological characteristics, perioperative outcomes, long-term prognostic outcomes PNI(+) PNI(-)...
Oxidative stress plays an important role in the development of colorectal cancer (CRC). This study is aimed at developing and validating a novel scoring system, based on oxidative indexes, for prognostic prediction CRC patients. A retrospective analysis 1422 patients who underwent surgical resection between January 2013 December 2017 was performed. These were randomly assigned to training set ( n = 1022) or validation 400). Cox regression model used analyze laboratory parameters. The...
Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, different survival outcomes. However, it is unclear whether gut microbiota between CRCs MMR status. In this study, we used immunohistochemistry for four major proteins determine the status in 230 CRC patients. The was profiled cancerous adjacent normal tissues by using bacterial 16S rRNA sequencing. differences diversity,...
Abstract Background Inflammatory, immune, and nutritional status are key factors in obstructive colorectal cancer (OCRC). This study aims to investigate the value of modified Naples prognostic score (M-NPS) evaluating OCRC prognosis. Methods A total 196 patients were retrospectively analyzed construct M-NPS based on serum albumin (ALB), cholesterol (CHOL), neutrophil:lymphocyte ratio (NLR), lymphocyte:monocyte (LMR), then they divided into three groups. The Kaplan–Meier (KM) method Cox...
Abstract Background Serum bilirubin and total bile acid (TBA) levels have been reported to be strongly associated with the risk prognosis of certain cancers. Here, we aimed investigate effects pretreatment serum acids on patients colorectal cancer (CRC). Methods A retrospective cohort 1474 CRC who underwent surgical resection between January 2015 December 2017 was included in study. Survival analysis used evaluate predictive value acids. X-Tile software identify optimal cut-off values for...
Despite the notable achievements of programmed death 1 (PD-1) antibodies in treating various cancers, overall efficacy remains limited majority colorectal cancer (CRC) cases. Metabolism reprogramming tumors inhibits tricarboxylic acid (TCA) cycle, leading to down-regulation fumarate hydratase (FH), which is related poor prognosis CRC patients. By establishing a tumor-bearing mouse model with Fh1 expression deficiency, we confirmed that therapeutic effect PD-1 alone was suboptimal mice low...
Abstract Background Colorectal cancer (CRC) patients with mismatch repair-deficient/microsatellite instability-high (dMMR/MSI-H) status are conventionally perceived as unresponsive to adjuvant chemotherapy (ACT). The mitochondrial transcription factor A (TFAM) is required for DNA copy number (mtDNA-CN) expression. In light of previous findings indicating that the frequent truncating-mutation TFAM affects resistance MSI CRC cells, this study aimed explore potential mtDNA-CN a predictive...
Arginase is abundantly expressed in colorectal cancer and disrupts arginine metabolism, promoting the formation of an immunosuppressive tumor microenvironment. This significant factor contributes to insensitivity immunotherapy. Tumor-associated macrophages (TAMs) are major immune cells this environment, aberrant metabolism tissues induces TAM polarization toward M2-like macrophages. The natural compound piceatannol 3'-O-glucoside inhibits arginase activity activates nitric oxide synthase,...
Abstract In the actual production of ultrasonic atomization, to cater consumers’ preference for appearance products, surface atomizer needs be treated with different machining processes, which tend affect hardness atomizer, and atomization performance atomizer. Therefore, beauty reduction has become a contradiction in current industrial atomizers. this study, it is theoretically proved that inversely proportional rate. Two processes are selected among five common processing techniques...
More and more steatotic livers undergo resection or transplantation but they exhibit higher susceptibility to ischemia-reperfusion injury (IRI), which results in increased perioperative complication morbidity mortality. IRI is driven by various cytokines receptors, both of are extensively modified N-glycosylation. We aim elucidate from the perspective Differentially expressed genes glycoproteins were identified with RNA-seq N-glycoproteomics. Myeloid LIF hepatocyte LIFR knockout mice...
<p>Differential expression analysis of the transcriptome NF and CAF in OCRC, CRC normal SMA positive tissues</p>
<p>Figure S17: Animal models validated vanillylacetone inhibited PA-induced cytokine secretion and NF-to-CAF/mCAF activation. (A-B) Measurement of CSF-1, CXCL8, TGF-β1 levels in CRC subcutaneous tumors serum C57BL/6 (A) BALB/c (B) mice post-vanillylacetone treatment. (C) Immunofluorescence staining analysis mCAFs treatment, green: FN1, red: α-SMA (Scale bars = 1mm 20μm). (D) FN1-positive CAFs content from Statistical significance was determined by unpaired Student t test. *P < 0.05,...
<p>Figure S15: Screening of natural compounds targeting the NF-κB pathway. (A-B) Validations potential via qRT‒PCR (A) and Western blotting (B). (C-D) ELISA assay confirmed that vanillylacetone (C) significantly inhibited CSF-1, CXCL8, TGF-β1 secretion CRC cells compared to pyrogallol (D). Statistical significance was determined by one-way two-way ANOVA. *P < 0.05, **P 0.01, ***P 0.001, ****P 0.0001, ns, no significance.</p>
<p>Figure S8: CAF content was associated with dyslipidemia and elevated intratumoral PA levels in OCRC. (A) Relationships between CAFscore OCRC incidence, serum lipid levels, glucose, arterial calcification atherosclerosis. (B-D) microvessel density (MVD) (B), microlymphatic (MLD) (C), EMAscore (D), lipids, Statistical significance determined by Chi-Squared Test, Spearman correlation used for statistical methods. *P < 0.05, **P 0.01, ***P 0.001, ****P 0.0001.</p>