A5006295332- Metalloenzymes and iron-sulfur proteins
- Trace Elements in Health
- Enzyme Structure and Function
- Enzyme Catalysis and Immobilization
- Metal-Catalyzed Oxygenation Mechanisms
- Porphyrin Metabolism and Disorders
- Iron Metabolism and Disorders
- Microbial Metabolic Engineering and Bioproduction
- Electrochemical sensors and biosensors
- Biochemical and Molecular Research
- Chemical Analysis and Environmental Impact
- Electrocatalysts for Energy Conversion
- Electrochemical Analysis and Applications
- Enzyme-mediated dye degradation
- Radioactive element chemistry and processing
- Metal complexes synthesis and properties
- Photosynthetic Processes and Mechanisms
- Analytical chemistry methods development
- Water Treatment and Disinfection
- Metal Extraction and Bioleaching
- Metabolism and Genetic Disorders
- Microbial Fuel Cells and Bioremediation
- Corrosion Behavior and Inhibition
- Mass Spectrometry Techniques and Applications
- Click Chemistry and Applications
Delft University of Technology
2016-2025
Wageningen University & Research
1998-2021
Faculty (United Kingdom)
2020
Biocat
2016
In-Q-Tel
2009-2011
Helmholtz Centre for Environmental Research
2010
Institut de Biologie Structurale
2007
Laboratoire de Chimie Bactérienne
2007
University of Cologne
2007
University of Georgia
2001-2002
En route to a bio-based chemical industry, the conversion of fatty acids into building blocks is particular interest. Enzymatic routes, occurring under mild conditions and excelling by intrinsic selectivity, are particularly attractive. Here we report photoenzymatic cascade reactions transform unsaturated enantiomerically pure secondary alcohols. In first step C=C-double bond stereoselectively hydrated using oleate hydratases from Lactobacillus reuteri or Stenotrophomonas maltophilia. Also,...
More than ten ergot alkaloids comprising both natural and semi-synthetic products are used to treat various diseases1,2. The central C ring forms the core pharmacophore for alkaloids, giving them structural similarity neurotransmitters, thus enabling their modulation of neurotransmitter receptors3. haem catalase chanoclavine synthase (EasC) catalyses construction this through complex radical oxidative cyclization4. Unlike canonical catalases, which catalyse H2O2 disproportionation5,6, EasC...
The Birch reduction of aromatic rings to cyclohexadiene compounds is widely used in chemical synthesis and requires solvated electrons, the most potent reductants known organic chemistry. Benzoyl-coenzyme A (CoA) reductases (BCR) are key enzymes anaerobic bacterial degradation catalyze an analogous reaction under physiological conditions. Class I BCRs FeS couple reductive dearomatization benzoyl-CoA cyclohexa-1,5-diene-1-carboxyl-CoA (dienoyl-CoA) a stoichiometric ATP hydrolysis. Here, we...
Laccases (EC 1.10.3.2) are members of the multicopper oxidase family. They oxidize diverse electron-rich substrates through electron abstraction by type 1 copper ion in enzyme active site. Abstracted electrons transferred to trinuclear cluster, where molecular oxygen serves as final acceptor and is reduced water. Laccase activity assumed depend on redox potential its ion. Whereas numerous studies have been undertaken elucidate determinants ions one-domain cupredoxins three-domain laccases,...
Abstract Development of synthetic bactericidal surfaces is a drug‐free route to the prevention implant‐associated infections. Surface nanotopographies with specific dimensions have been shown kill various types bacterial strains through mechanical mechanism, while regulating stem cell differentiation and tissue regeneration. The effective ranges required simultaneously achieve both aims are in <200 nm range. Here, nanoscale additive manufacturing (=3D printing) technique called electron...
The combination of UV/visible/NIR absorption, CD and variable-temperature magnetic circular dichroism (VTMCD), EPR, X-ray absorption (XAS) spectroscopies has been used to investigate the electronic structural properties oxidized reduced forms Pyrococcus furiosus superoxide reductase (SOR) as a function pH exogenous ligand binding. XAS shows that mononuclear ferric center in enzyme is very susceptible photoreduction beam. This observation facilitates interpretation ground- excited-state EXAFS...
Two formate dehydrogenases (CO2-reductases) (FDH-1 and FDH-2) were isolated from the syntrophic propionate-oxidizing bacterium Syntrophobacter fumaroxidans. Both enzymes produced in axenic fumarate-grown cells as well which grown syntrophically on propionate with Methanospirillum hungatei H2 scavenger. The purified exhibited extremely high formate-oxidation CO2-reduction rates, low Km values for formate. For enzyme designated FDH-1, a specific oxidation rate of 700 U.mg-1 0.04 mm measured...
The hyperthermophile Pyrococcus furiosus catalyses the hydrogenation of a broad range carboxylic acids selectively to corresponding primary alcohols. Other functional groups such as isolated C=C-double bonds are not touched. chemoselectivity carboxylate reduction may be directed towards aldehydes by simple medium engineering.
Laccases have gained significant attention due to their emerging applications including bioremediation, biomass degradation and biofuel cells. One of the prerequisites for industrial application laccases is sufficient availability. However, expression levels recombinantly expressed are often low. In this study Mrl2, a new laccase from basidiomycete Moniliophthora roreri, was cloned in Pichia pastoris produced an optimized fed-batch process at exceptionally high yield 1.05 g l-1. With redox...
One of the major problems with bone implant surfaces after surgery is competition host and bacterial cells to adhere surfaces. To keep implants safe against implant-associated infections, surface may be decorated bactericidal nanostructures. Therefore, fabrication nanostructures on biomaterials growing interest. Here, we systematically studied effects different processing parameters inductively coupled plasma reactive ion etching (ICP RIE) Ti The resultant were characterized by using...
Abstract The amino‐terminal copper and nickel/N‐terminal site (ATCUN/NTS) present in proteins bioactive peptides exhibits high affinity towards Cu II ions have been implicated human physiology. Little is known, however, about the rate exact mechanism of formation such complexes. We used stopped‐flow microsecond freeze‐hyperquenching (MHQ) techniques supported by steady‐state spectroscopic electrochemical data to demonstrate partially coordinated intermediate complexes formed...
ABSTRACT A novel tungstate and molybdate binding protein has been discovered from the hyperthermophilic archaeon Pyrococcus furiosus . This transport (WtpA) is part of a new ABC transporter system selective for molybdate. WtpA very low sequence similarity with earlier-characterized proteins ModA TupA tungstate. Its structural gene present in genome numerous archaea some bacteria. The identification this clarifies mechanism organisms that lack known uptake systems associated proteins, like...
Post Synthetic Modification (PSM) of amino-metal organic frameworks (NH<sub>2</sub>-MOFs) with the enzyme Glucose Oxidase (GOx) is reported.
Abstract A highly conserved tyrosine residue of unknown function is present in the vicinity di‐iron catalytic center ubiquitous iron‐storage protein ferritin. The with a gateway Fe II /Fe III ‐binding site nearby provides vital mechanism protein. It believed that, eukaryotic ferritin, this catalyzes simultaneous oxidation two ions, whereas microbial ferritin it three ions. To understand role tyrosine, we studied intermediates and products that are formed during catalysis centers...
Abstract Supramolecular encapsulation is known to alter chemical properties of guest molecules. We have applied this strategy molecular temporally control the catalytic activity a stable copper(I)–carbene catalyst. Encapsulation catalyst by supramolecular host cucurbit[7]uril (CB[7]) resulted in complete inactivation copper‐catalyzed alkyne–azide cycloaddition (CuAAC) reaction. The addition signal achieved near instantaneous activation catalyst, releasing from inhibited CB[7] complex. To...
Arabidopsis thaliana hydroxynitrile lyase (AtHNL) catalyzes the selective synthesis of (R)-cyanohydrins. This enzyme is unstable under acidic conditions, therefore its immobilization necessary for enantiopure cyanohydrins. EziG Opal a controlled porosity glass material His-tagged enzymes. The His6-tagged AtHNL on was optimized higher stability and tested (R)-mandelonitrile in batch continuous flow systems. AtHNL-EziG achieved 95% conversion after 30 min reaction time it recycled up to eight...