A5074039258- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Plant biochemistry and biosynthesis
- Fungal Biology and Applications
- Gastric Cancer Management and Outcomes
- Natural product bioactivities and synthesis
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Various Chemistry Research Topics
- Microfluidic and Capillary Electrophoresis Applications
- Metabolomics and Mass Spectrometry Studies
- Fecal contamination and water quality
- Genetic factors in colorectal cancer
- Marine Sponges and Natural Products
- 14-3-3 protein interactions
- Glycosylation and Glycoproteins Research
- Helicobacter pylori-related gastroenterology studies
- Infection Control in Healthcare
Ecolab (United Kingdom)
2018
St. Olaf College
2009-2017
Northwestern University
2012-2016
University of Wisconsin–Madison
2015
For more than half a century the pharmaceutical industry has sifted through natural products produced by microbes, uncovering new scaffolds and fashioning them into broad range of vital drugs. We sought strategy to reinvigorate discovery with distinctive structures using bacterial genome sequencing combined metabolomics. By correlating genetic content from 178 actinomycete genomes mass spectrometry-enabled analyses their exported metabolomes, we paired secondary metabolites biosynthetic gene...
The microbial world offers a rich source of bioactive compounds for those able to sift through it. Technologies capable quantitatively detecting natural products while simultaneously identifying known would expedite the search new pharmaceutical leads. Prior efforts have targeted histone deacetylases in fungi globally activate production secondary metabolites, yet no study has directly assessed its effects with minimal bias at metabolomic level. Using untargeted metabolomics, we monitored...
With thousands of fungal genomes being sequenced, each genome containing up to 70 secondary metabolite (SM) clusters 30-80 kb in size, breakthrough techniques are needed characterize this SM wealth.Here we describe a novel system-level methodology for unbiased cloning intact large from single one-step transformation and expression model host. All 56 Aspergillus terreus were individually captured self-replicating artificial chromosomes (FACs) both the E. coli F replicon an autonomously...
"Omic" strategies have been increasingly applied to natural product discovery processes, with (meta-)genome sequencing and mining implemented in many laboratories date. Using the proteomics-based platform called PrISM (Proteomic Investigation of Secondary Metabolism), we discovered two new siderophores gobichelin A B from Streptomyces sp. NRRL F-4415, a strain without sequenced genome. proteomics information as guide, 37 kb gene cluster responsible for production gobichelins was its 20 open...
Abstract Matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry is an important bioanalytical technique in drug discovery, proteomics, and research at the biology‐chemistry interface. This especially powerful tool when combined with gel separation of proteins database mining using spectral data. Currently, few hands‐on laboratory opportunities exist for undergraduate students to master this despite usefulness biological research. One reason lack incorporation into teaching...
Capillary electrophoresis is an important analytical separation method used to study a wide variety of samples, including those biological origin. may be covered in the classroom, especially advanced courses, and while many students are exposed gel biology or biochemistry laboratories, capillary not as commonly found undergraduate lab curricula. Published experiments that incorporate include analysis caffeine beverages cations water samples but more such peptide mixture. This paper...