A5054210273- Pancreatic function and diabetes
- Pancreatitis Pathology and Treatment
- Diabetes and associated disorders
- Diabetes Treatment and Management
- Blood properties and coagulation
- Diabetes Management and Research
- Metabolism, Diabetes, and Cancer
- Spectroscopy Techniques in Biomedical and Chemical Research
- 3D Printing in Biomedical Research
- Body Contouring and Surgery
- Pancreatic and Hepatic Oncology Research
- Renal Transplantation Outcomes and Treatments
University of Oxford
2018-2024
John Radcliffe Hospital
2018-2024
Oxford Centre for Diabetes, Endocrinology and Metabolism
2018-2023
Nuffield Orthopaedic Centre
2023
Churchill Hospital
2018-2019
Using
Despite huge advances in the field of islet transplantation over last two decades, current isolation methods remain suboptimal, with transplantable yields obtained less than half all pancreases processed worldwide. Successful is dependent on ability collagenase-based enzyme blends to digest extracellular matrix components at islet-exocrine interface. The limited availability donor hinders use full-scale isolations characterize pancreas digestion by different or blends, allow influence...
ABSTRACT Using 13 C 6 glucose labeling coupled to GC-MS and 2D 1 H- HSQC NMR spectroscopy, we have obtained a comparative high-resolution map of fate underpinning β cell function. In both mouse human islets, the contribution TCA cycle is similar. Pyruvate-fueling primarily mediated by activity pyruvate dehydrogenase, with lower flux through carboxylase. While conversion lactate dehydrogenase (LDH) can be detected in islets species, accumulation six-fold higher islets. Human express LDH,...
Introduction: Total Pancreatectomy with Islet Auto-Transplantation (TPIAT) is an effective treatment for selected patients severe chronic pancreatitis, resulting in pain reduction, improved quality of life, and good graft function following islet re-infusion. Islets are isolated using a blend collagenase neutral protease (NP) enzymes. Most isolation labs use high concentrations NP auto (~200 Units), due to the fibrotic nature retrieved pancreases. However, increasing can result fragmentation...
Introduction: The islet basement membrane (BM) is a specialized sheet of extracellular matrix comprised primarily laminins, collagen IV and perlecan. It essential for function survival. During the enzymatic digestion phase isolation, BM significantly disrupted. Further loss occurs during mandatory culture period, when islets are placed in CMRL media at 37°C. Previous studies have demonstrated superiority UW cold storage solution maintaining post-isolation period. However, impact on integrity...
The UK Islet Transplant Consortium (UKITC). Pancreas Advisory Group Sub-Group (PAGISG). Introduction: transplantation success is influenced by the mass of infused insulin producing tissue, resulting in many islet transplant programs employing a minimum dose 5,000 IEQ/Kg for first transplants. Donor-specific factors (such as age, BMI, and past medical history) affect function. Similarly, recipient pre-transplant duration diabetes) may influence success. However, current dosing strategies do...