A5037837022- Transgenic Plants and Applications
- Protein purification and stability
- Viral Infectious Diseases and Gene Expression in Insects
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Genomics and Chromatin Dynamics
- Virus-based gene therapy research
- Wound Healing and Treatments
- Epigenetics and DNA Methylation
- Animal Genetics and Reproduction
- Orthopedic Infections and Treatments
Leidos (United States)
2015-2018
Cell-free protein synthesis has emerged as a powerful technology for rapid and efficient production. methods are also amenable to automation such systems have been extensively used high-throughput production screening; however, current fluidic not adequate manufacturing biopharmaceuticals. In this work, we report on the initial development of process end-to-end recombinant biologics. This incorporates bioreactor module that can be with eukaryotic or prokaryotic lysates programmed combined...
Genetically modified organisms (GMOs) have numerous biomedical, agricultural and environmental applications. Development of accurate methods for the detection GMOs is a prerequisite identification control authorized unauthorized release these engineered into environment food chain. Current are unable to detect uncharacterized GMOs, since either DNA sequence transgene or amino acid protein must be known DNA-based immunological-based detection, respectively.Here we describe application an...
There is an established relationship between primary DNA sequence, secondary and tertiary chromatin structure, transcriptional activity, suggesting that observed differences in one of these properties may reflect changes the others. Here, we exploit relationships to show variations structure can be used identify a wide range genomic alterations mammalian samples. In this proof-of-concept study characterized compared genome-wide histone occupancy by ChIP-Seq, accessibility ATAC-Seq,...
Identification of wound-specific markers would represent an important step toward damaged tissue detection and targeted delivery biologically materials to injured sites. Such could minimize the amount therapeutic that must be administered limit potential collateral damage on nearby normal tissues. Yet, biological are specific for sites remain elusive. In this study, we have developed immunohistological approach identification protein epitopes specifically exposed in wounded Using ex-vivo...