A5068312032- Congenital heart defects research
- Developmental Biology and Gene Regulation
- Cancer-related gene regulation
- Heterotopic Ossification and Related Conditions
- dental development and anomalies
- TGF-β signaling in diseases
- Medical Imaging and Pathology Studies
- Sperm and Testicular Function
- Epigenetics and DNA Methylation
- Animal Genetics and Reproduction
- Bone Tumor Diagnosis and Treatments
- RNA modifications and cancer
- Genetic Syndromes and Imprinting
- RNA Interference and Gene Delivery
- Craniofacial Disorders and Treatments
- Renal and related cancers
- Oral and Maxillofacial Pathology
- Tissue Engineering and Regenerative Medicine
- Zebrafish Biomedical Research Applications
- Bone Metabolism and Diseases
- Dental Research and COVID-19
- Wnt/β-catenin signaling in development and cancer
- Genetics and Neurodevelopmental Disorders
- Xenotransplantation and immune response
- interferon and immune responses
Tufts University
2015-2024
American Dental Education Association
2022
John Wiley & Sons (United States)
2022
Boston University
2012
Georgia Institute of Technology
2005
University of New Hampshire
2005
University of California, Irvine
2002
Novant Health Forsyth Medical Center
1995-1998
Harvard University
1991
University of Turku
1988
African cichlid fishes have repeatedly evolved highly specialized modes of feeding through adaptations their oral jaws. Here, we explore the molecular genetic basis opening and closing lever mechanisms lower jaw, which traditionally been used to describe mechanics behavior in bony fishes. Quantitative analyses demonstrate that are genetically modular therefore free evolve independently. Bmp4 ( bone morphogenetic protein 4 ) is one two loci segregate with mechanical advantage together account...
Biased left-right asymmetry is a fascinating and medically important phenomenon. We provide molecular genetic physiological characterization of novel, conserved, early, biophysical event that crucial for correct asymmetry: H+ flux. A pharmacological screen implicated the H+-pump H+-V-ATPase in Xenopus asymmetry, where it acts upstream early asymmetric markers. Immunohistochemistry revealed an actin-dependent subunits during first three cleavages. H+-flux across plasma membranes also at four-...
Pulpal revascularization is commonly used in the dental clinic to obtain apical closure of immature permanent teeth with thin dentinal walls. Although sometimes successful, stimulating bleeding from periapical area tooth can be challenging and turn may deleteriously affect root maturation. Our objective here was define reliable methods regenerate pulp-like tissues segments (RSs). G1 RSs were injected human pulp stem cells (hDPSCs) umbilical vein endothelial (HUVECs) encapsulated 5% gelatin...
Key points Xenopus laevis craniofacial development is a good system for the study of Andersen–Tawil Syndrome (ATS)‐associated anomalies (CFAs) because (1) Kcnj2 expressed in nascent face; (2) molecular‐genetic and biophysical techniques are available ion‐dependent signalling during morphogenesis; (3) as humans, expression variant forms embryos causes muscle phenotype; (4) found human patients, when injected into frog embryos, cause CFAs same cell lineages. Forced WT or changes normal pattern...
Idiopathic scoliosis (IS) is the most common spinal deformity diagnosed in childhood or early adolescence, while underlying pathogenesis of this serious condition remains largely unknown. Here, we report zebrafish ccdc57 mutants exhibiting during late development, similar to that observed human adolescent idiopathic (AIS). Zebrafish developed hydrocephalus due cerebrospinal fluid (CSF) flow defects caused by uncoordinated cilia beating ependymal cells. Mechanistically, Ccdc57 localizes...
Abstract A gene encoding a possible novel human cathepsin, cysteine proteinase that is distinct from previously characterized enzymes, has been identified by differential screening of osteoclastoma cDNA library. This molecule, termed cathepsin X, appears to represent the homolog osteoclast-expressed rabbit OC-2. Cathepsin X (GenBank accession number U20280) 93.9% identical OC-2 at amino acid level, and 92% nucleotide level within coding region. 52.2 46.9% cathepsins S L, respectively,...
During vertebrate craniofacial development, neural crest cells (NCCs) contribute to most of the pharyngeal skeleton. Defects in NCC specification, migration and differentiation resulting malformations complex are associated with human disorders including Treacher-Collins Syndrome, caused by mutations TCOF1. It has been hypothesized that perturbed ribosome biogenesis p53 mediated neuroepithelial apoptosis results hypoplasia mouse Tcof1 mutants. However, underlying mechanisms linking...
To date, in mammals except for the mouse and human, only one protamine variant has been isolated from sperm. These mammalian protamines share amino acid sequence homology with 1 (mP1), tyrosine-containing variant. Southern blot analysis of restriction enzyme digests hamster rat liver DNA reveals presence sequences homologous to mP1, also 2 (mP2) cDNAs. Northern blots total testis RNA probed mP2 cDNA confirm that gene these species is transcribed into two size classes mRNA approximately 830...
Sequence analysis of a mouse testicular alpha-tubulin partial cDNA, pRD alpha TT1, reveals an isotype that differs from both the somatic and predominant tubulins at approximately 30% 212 amino acid residues determined. Although this cDNA retains highly conserved sequence, Glu-Gly-Glu-Glu, found in carboxyl termini many tubulins, protein extends substantially beyond sequence does not terminate with C-terminal tyrosine. Using rabbit antiserum prepared to novel synthetic peptide predicted...
Abstract Transition protein 1 (TP1) is a small basic nuclear that functions in chromatin condensation during spermatogenesis mammals. Here, recently identified cDNA clones encoding mouse transition 1(mTP1) were used to characterize the expression of mTP1 mRNA spermatogenesis. Southern blot analysis demonstrates there single copy gene for genome. Northern polyadenylated approximately 600 bases long, which first detected at round spermatid stage not detectable poly(A)+ RNAs isolated from...
A mouse protamine 1 cDNA probe was used to study P1 gene expression during the cycle of seminiferous epithelium in rat. In situ hybridization experiments showed that transcription mRNA starts middle step 7 spermiogenesis substage VIIc. The levels stay high steps 7-14 spermatids but decrease 15-16 and are virtually undetectable 17-19 spermatids. Northern blot analyses RNAs isolated from microdissected pools tubules show concentrations stages VIIc-XIV-III lower IV-VIIb. Owing a...
Fibrodysplasia ossificans progressiva (FOP) is a rare, autosomal dominant genetic disorder in humans characterized by explosive inflammatory response to injury leading gradual ossification within fibrous tissues, including skeletal muscle, tendons, and ligaments. A variety of animal models are needed study understand the etiology human FOP. To address this need, here we present characterizations first adult zebrafish model for In humans, activating mutations Type I BMP/TGFβ family member...
Abstract Zebrafish craniofacial, skeletal, and tooth development closely resembles that of higher vertebrates. Our goal is to identify viable adult zebrafish mutants can be used as models for human mineralized dental, skeletal system disorders. We a large‐scale forward‐genetic chemical N ‐ethyl‐nitroso‐urea mutagenesis screen 17 early lethal homozygous recessive with defects in craniofacial cartilage elements, 7 tissue phenotypes including shape defects, fused sutures, dysmorphic or missing...
Abstract Zebrafish E‐cadherin ( cdh1 ) cell adhesion molecule cDNAs were cloned. We investigated spatial and temporal expression of during early embryogenesis. Expression was observed in blastomeres, the anterior mesoderm gastrulation, developing epithelial structures. In nervous system, detected at pharyngula stage (24 hpf) midbrain‐hindbrain boundary (MHB). Developmental regulation MHB formation involves wnt1 pax2.1 . preceded formation, dependent on normal development this structure. ©...