A5088757113- Evolution and Genetic Dynamics
- Neurogenetic and Muscular Disorders Research
- Helicobacter pylori-related gastroenterology studies
- Cellular transport and secretion
- CRISPR and Genetic Engineering
- Gastrointestinal motility and disorders
- Innovative Microfluidic and Catalytic Techniques Innovation
- Antibiotic Resistance in Bacteria
- Virus-based gene therapy research
- Lysosomal Storage Disorders Research
- Pharmaceutical and Antibiotic Environmental Impacts
- Gut microbiota and health
University of Amsterdam
2021-2024
Erasmus MC
2023
Netherlands Institute for Neuroscience
2021
Resistance evolution during exposure to non-lethal levels of antibiotics is influenced by various stress responses bacteria which are known affect growth rate. Here, we aim disentangle how the interplay between resistance development and associated fitness costs affected responses. We performed de novo wild-type strains single-gene knockout in response pathways using four different antibiotics. Throughout development, increase minimum inhibitory concentration (MIC) accompanied a gradual...
Mucopolysaccharidosis type II (OMIM 309900) is a lysosomal storage disorder caused by iduronate 2-sulfatase (IDS) deficiency and accumulation of glycosaminoglycans, leading to progressive neurodegeneration. As intravenously infused enzyme replacement therapy cannot cross the blood-brain barrier (BBB), it fails treat brain pathology, highlighting unmet medical need develop alternative therapies. Here, we test modified versions hematopoietic stem progenitor cell (HSPC)-mediated lentiviral gene...
Resistance evolution during exposure to non-lethal levels of antibiotics is influenced by various stress responses bacteria which are known affect growth rate. Here, we aim disentangle how the interplay between resistance development and associated fitness costs affected responses. We performed de novo wild-type strains single-gene knockout in response pathways using four different antibiotics. Throughout development, increase minimum inhibitory concentration (MIC) accompanied a gradual...
Deficiency of iduronate 2-sulfatase (IDS) causes Mucopolysaccharidosis type II (MPS II), a lysosomal storage disorder characterized by systemic accumulation glycosaminoglycans (GAGs), leading to devastating cognitive decline and life-threatening respiratory cardiac complications. We previously found that hematopoietic stem progenitor cell-mediated lentiviral gene therapy (HSPC-LVGT) employing tagged IDS with insulin-like growth factor 2 (IGF2) or ApoE2, but not receptor-associated protein...