A5049805180- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- RNA and protein synthesis mechanisms
- Cytomegalovirus and herpesvirus research
Stanford University
2022-2024
Abstract CRISPR perturbation methods are limited in their ability to study non-coding elements and genetic interactions. In this study, we developed a system for bidirectional epigenetic editing, called CRISPRai, which apply activating (CRISPRa) repressive (CRISPRi) perturbations two loci simultaneously the same cell. We CRISPRai Perturb-seq by coupling dual gRNA detection with single-cell RNA sequencing, enabling of pooled mixed population. applied platform interaction between hematopoietic...
ABSTRACT CRISPR perturbations are valuable tools for studying the functional effects of genome. However, existing methods limited in their utility noncoding elements and genetic interactions. Here, we develop a system bidirectional epigenetic editing (CRISPRai), which orthogonal activating (CRISPRa) repressive (CRISPRi) applied simultaneously to multiple loci same cell. We developed dual-gRNA-capture single-cell Perturb-seq study established interaction between SPI1 GATA1, two hemopoietic...