A5042426323- Antimicrobial Resistance in Staphylococcus
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Bacillus and Francisella bacterial research
- RNA Research and Splicing
- Biochemical and Structural Characterization
- Plant Pathogenic Bacteria Studies
- Olfactory and Sensory Function Studies
- Microbial Metabolism and Applications
- Antifungal resistance and susceptibility
- Advanced biosensing and bioanalysis techniques
- Signaling Pathways in Disease
- Nanoparticle-Based Drug Delivery
- Bacterial Identification and Susceptibility Testing
- Acne and Rosacea Treatments and Effects
- RNA Interference and Gene Delivery
Laboratoire de Microbiologie et Génétique Moléculaires
2021-2024
Université Toulouse III - Paul Sabatier
2021-2023
Université de Toulouse
2023
Universidade Nova de Lisboa
2015-2023
Instituto de Tecnología Química
2019
University Hospital of Geneva
2011-2016
Université de Rennes
2009-2011
Inserm
2009-2011
ABSTRACT The development and maintenance of an arsenal antibiotics is a major health care challenge. Ceftaroline new cephalosporin with activity against methicillin-resistant Staphylococcus aureus (MRSA); however, no reports concerning MRSA ceftaroline susceptibility have been reported in Switzerland. We tested the vitro archived set 60 strains from University Hospital Geneva collected 1994 to 2003. Our results surprisingly revealed ceftaroline-resistant (MIC, >1 μg/ml 40/60 strains;...
ABSTRACT Expression of the methicillin-resistant S. aureus (MRSA) phenotype results from expression extra penicillin-binding protein 2A (PBP2A), which is encoded by mecA and acquired horizontally on part SCC mec cassette. PBP2A can catalyze dd -transpeptidation peptidoglycan (PG) because its low affinity for β-lactam antibiotics functionally cooperate with PBP2 transglycosylase in biosynthesis PG. Here, we focus upon role membrane-bound PrsA foldase as a regulator resistance expression....
Abstract Unregulated cell cycle progression may have lethal consequences and therefore, bacteria various mechanisms in place for the precise spatiotemporal control of events. We uncovered a new link between chromosome replication/segregation splitting division septum. show that DNA translocase domain‐containing divisome protein FtsK regulates cellular levels peptidoglycan hydrolase Sle1, which is involved separation bacterial pathogen Staphylococcus aureus . interacts with chaperone (trigger...
The precise mechanisms leading to the emergence of low-level glycopeptide resistance in Staphylococcus aureus are poorly understood. In this study, we used whole genome deep sequencing detect differences between two isogenic strains: a parental strain and stable derivative selected stepwise for survival on 4 µg/ml teicoplanin, but which grows at higher drug concentrations (MIC 8 µg/ml). We uncovered only three single nucleotide changes strain. Nonsense mutations occurred stp1, encoding...
ABSTRACT Understanding in detail the factors which permit Staphylococcus aureus to counteract cell wall-active antibiotics is a prerequisite elaborating effective strategies prolong usefulness of these drugs and define new targets for pharmacological intervention. Methicillin-resistant S. (MRSA) strains are major pathogens hospital-acquired community-acquired infections most often treated with glycopeptides (vancomycin teicoplanin) because their resistance penicillins limited arsenal...
β-lactam antibiotics interfere with cross-linking of the bacterial cell wall, but killing mechanism this important class is not fully understood. Serendipitously we found that sub-lethal doses β-lactams rescue growth and prevent spontaneous lysis Staphylococcus aureus mutants lacking widely conserved chaperone ClpX, reasoned a better understanding clpX phenotypes could provide novel insights into downstream effects binding to PBP targets. Super-resolution imaging revealed cells display...
ABSTRACT S. aureus combats cell wall antibiotic stress by altered gene expression mediated various environmental signal sensors. In this study, we examined the transcriptional regulation of trfA , a related to mecA Bacillus subtilis encoding an adaptor protein implicated in multiple roles, notably, proteolysis and genetic competence. Despite strong sequence similarity B. function remains largely unexplored; however, its deletion leads almost complete loss resistance oxacillin glycopeptide...
Staphylococcus aureus is an important pathogen manifesting virulence through diverse disease forms, ranging from acute skin infections to life-threatening bacteremia or systemic toxic shock syndromes. In the latter case, prototypical superantigen TSST-1 (Toxic Shock Syndrome Toxin 1), encoded by tst(H), and carried on a mobile genetic element that not present in all S. strains. Transcriptional regulation of tst only partially understood. this study, we dissected role sarA, sarS (sarH1),...
Cellular processes require precise and specific gene regulation, in which continuous mRNA degradation is a major element. The mechanisms should be able to degrade wide range of different RNA substrates with high efficiency, but at the same time limited, avoid killing cell by elimination all cellular RNA. RNase Y endoribonuclease found most Firmicutes, including Bacillus subtilis Staphylococcus aureus. However, molecular interactions that direct cleave correct molecules position remain...
Staphylococcus aureus is capable of causing a remarkable spectrum disease, ranging from mild skin eruptions to life-threatening infections. The survival and pathogenic potential S. depend partly on its ability sense respond changes in environment. Spx thiol/oxidative stress sensor that interacts with the C-terminal domain RNA polymerase RpoA subunit, leading gene expression help sustain viability under various conditions. Using genetic deep-sequencing methods, we show spx essential...
PBP4 is typically considered unimportant for conferring high-level β-lactam resistance in Staphylococcus aureus. Mutations have been associated with non-susceptibility among natural strains of S. We previously shown that can mediate laboratory-generated passaged antibiotics. the pbp4 promoter up-regulate its expression and missense mutations surround PBP4's active site were detected high frequencies strains, suggesting plays a key role resistance. How these participate ability to provide...
Plasmids need to ensure their transmission both daughter-cells when host divides, but should at the same time avoid overtaxing hosts by directing excessive host-resources toward production of plasmid factors. Naturally occurring plasmids have therefore evolved regulatory mechanisms restrict copy-number in response volume cytoplasm. In many families, control is mediated a small plasmid-specified RNA, which continuously produced and rapidly degraded, that its concentration proportional current...
Abstract Cellular processes require precise and specific gene regulation, in which continuous mRNA degradation is a major element. The mechanisms should be able to degrade wide range of different RNA substrates with high efficiency, but at the same time limited, avoid killing cell by elimination all cellular RNA. RNase Y endoribonuclease found most Firmicutes, including Bacillus subtilis Staphylococcus aureus . However, molecular interactions that direct cleave correct molecules position...
Peptidoglycan (PGN) is the major component of bacterial cell wall, a structure essential for physical integrity and shape cell. Bacteria maintain by directing PGN incorporation to distinct regions cell, namely through localisation late stage synthesis proteins. These include two key protein families, SEDS transglycosylases bPBP transpeptidases, proposed function in cognate pairs. Rod-shaped bacteria have SEDS-bPBP pairs, involved elongation division. Here, we elucidate why coccoid bacteria,...