A5007281408- Plant responses to water stress
- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Autophagy in Disease and Therapy
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Cell death mechanisms and regulation
- Mitochondrial Function and Pathology
- Plant Pathogens and Fungal Diseases
- Postharvest Quality and Shelf Life Management
- Plant Parasitism and Resistance
- Polysaccharides and Plant Cell Walls
- Phytochemicals and Antioxidant Activities
- Plant Diversity and Evolution
- Plant Genetic and Mutation Studies
- Coastal wetland ecosystem dynamics
- Phytochemistry and Bioactivity Studies
- Soil Carbon and Nitrogen Dynamics
- Turfgrass Adaptation and Management
- Plant Surface Properties and Treatments
- Antioxidant Activity and Oxidative Stress
- Insect Resistance and Genetics
- Heat shock proteins research
Dalhousie University
2013-2024
Agriculture and Agri-Food Canada
2009
University of Toronto
2004-2007
University of Guelph
2007
University of Peradeniya
2001-2004
Oxford Brookes University
2001
Abstract Programmed cell death (PCD) functions in the developmental remodeling of leaf shape higher plants, a process analogous to digit formation vertebrate limb. In this study, we provide cytological characterization time course events as PCD remodels young expanding leaves lace plant. Tonoplast rupture is first event system, indicated by alterations cytoplasmic streaming, loss anthocyanin color, and ultrastructural appearance. Nuclei become terminal deoxynucleotidyl transferase–mediated...
Summary Programmed cell death (PCD) is fundamentally important for plant development, abiotic stress responses and immunity, but our understanding of its regulation remains fragmented. Building a stronger research community required to accelerate progress in this area through knowledge exchange constructive debate. In Viewpoint, we aim initiate collective effort integrate data across diverse set experimental models facilitate characterisation the fundamental mechanisms underlying PCD...
Abstract Aerenchyma formation in roots of maize ( Zea mays L.) involves programmed death cortical cells that is promoted by exogenous ethylene (1 µL L −1 ) or endogenous produced response to external oxygen shortage (3%, v/v). In this study, evidence degeneration the cell wall accompanies apoptotic‐like changes previously observed cytoplasm and nucleus (Gunawardena et al . Planta 212, 205–214, 2001), has been sought examining de‐esterified pectins (revealed monoclonal antibody JIM 5),...
Programmed cell death (PCD) is a suicide mechanism adopted by multicellular organisms that essential for development and resistance to different forms of stress. In plants, PCD involved from embryogenesis the whole plant. genetically regulated molecular pathways in this process animals are relatively more understood than plants. At morphological level, apoptosis, one animals, plant have some similarities such as shrinkage, shrinkage nucleus, DNA fragmentation. Because characteristics product...
Abstract Background Developmentally regulated programmed cell death (PCD) is the controlled of cells that occurs throughout life cycle both plants and animals. The lace plant ( Aponogeton madagascariensis ) forms perforations between longitudinal transverse veins in spaces known as areoles, via developmental PCD; begins center these areoles develops towards margin, creating a gradient PCD. This was examined using long- short-term live imaging, addition to histochemical staining, order...
An unusual form of leaf morphogenesis occurs in the aquatic, lace plant, Aponogeton madagascariensis (Aponogetonaceae). Early development, discrete patches cells undergo programmed cell death (PCD) and perforations during expansion. In addition to protoplasts, walls dying are degraded PCD. The cuticle perforation site is eroded first, followed by dissolution wall matrix components, so that appear as loose fibrillar networks form. Gel diffusion assays wall-degrading enzyme activity indicated...
Lace plant leaves utilize programmed cell death (PCD) to form perforations during development. The role of heat shock proteins (Hsps) in PCD lace leaf development is currently unknown. Hsp70 amounts were measured throughout development, and the results indicate that it highest before PCD. Increased correlate with raised anthocyanin content caspase-like protease (CLP) activity. To investigate effects on whole plants treated either known regulators [reactive oxygen species (ROS) or...
Programmed cell death (PCD) is required for many morphological changes, but in plants it has been studied much less detail than animals. The unique structure and physiology of the lace plant (Aponogeton madagascariensis) well suited vivo study developmental PCD. Live streaming video quantitative analysis, coupled with transmission electron microscopy, were used to better understand PCD sequence, an emphasis on chloroplasts. Dividing, dumbbell-shaped chloroplasts persisted until late stages...
Programmed cell death (PCD) is the regulated of cells within an organism. The lace plant (Aponogeton madagascariensis) produces perforations in its leaves through PCD. consist a latticework longitudinal and transverse veins enclosing areoles. PCD occurs at center these areoles progresses outwards, stopping approximately five from vasculature. role mitochondria during has been recognized animals; however, it less studied plants.The following paper elucidates mitochondrial dynamics...
Aponogeton madagascariensis produces perforations over its leaf surface via programmed cell death (PCD). PCD begins between longitudinal and transverse veins at the center of spaces regarded as areoles, continues outward, stopping several cells from these veins. The gradient that exists within a single areole leaves in an early stage development was used model to investigate cellular dynamics during PCD. Mitochondria have interactions with family proteases known caspases, actin cytoskeleton...
Although a majority of monocotyledons have simple leaves, pinnately or palmately dissected blades are found in four orders, the Alismatales, Pandanales, Dioscoreales and Arecales. Independent evolutionary origins leaf dissection indicated by phylogenetic analyses reflected diversity mechanisms employed during development. The mechanism blastozone fractionation through localized enhancement suppression growth free margin primordium occurs Araceae Dioscoreaceae. By contrast, corrugated, leaves...
Thilakarathna, R. M. S., Papadopoulos, Y. A., Rodd, A. V., Gunawardena , N., Fillmore, S. E. and Prithiviraj, B. 2012. Characterizing nitrogen transfer from red clover populations to companion bluegrass under field conditions. Can. J. Plant Sci. 92: 1163–1173. The ability of two (Trifolium pratense L.) cultivars, AC Christie (diploid) Tempus (tetraploid), fixed (N) (Poa pratensis was evaluated samples were harvested three times during the 2009 growing season N cultivars determined using...
The lace plant, Aponogeton madagascariensis, is an aquatic monocot that forms perforations in its leaves as part of normal leaf development. Perforation formation occurs through developmentally regulated programmed cell death (PCD). molecular basis PCD regulation the plant unknown, however ethylene has been shown to play a significant role. In this study, we examined role receptors during perforation formation. We isolated three AmERS1a, AmERS1b and AmERS1c. Using quantitative PCR, their...
Programmed cell death (PCD) plays an important role in several plant developmental processes. The phytohormone ethylene has been implicated PCD signalling many systems, but it is also processes such as seed germination, flowering, and climacteric fruit ripening. Lace (Aponogeton madagascariensis (Mirbel) H. Bruggen) aquatic monocot that develops perforated leaves via the deletion of cells through developmentally regulated PCD. ideal for studying PCD; however, little known about regulation...
Programmed cell death (PCD) is an important process for the development and maintenance of multicellular eukaryotes. In animals, there are three morphologically distinct types: apoptosis, autophagic death, necrosis. The search all-encompassing classification system based on plant morphology continues. lace a model studying PCD as leaf perforations form predictably via this during development. This study induced in cells that do not undergo developmental using various degrees types stress...