A5002941890- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Ovarian function and disorders
- Reproductive Health and Technologies
- Monoclonal and Polyclonal Antibodies Research
- Epigenetics and DNA Methylation
- Complement system in diseases
- Genetics and Neurodevelopmental Disorders
- Immunodeficiency and Autoimmune Disorders
- Cell Adhesion Molecules Research
- Chronic Lymphocytic Leukemia Research
- RNA Research and Splicing
- Cancer-related gene regulation
- Lung Cancer Treatments and Mutations
- RNA modifications and cancer
- Gastric Cancer Management and Outcomes
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Hemophilia Treatment and Research
- Cellular Mechanics and Interactions
- Nerve injury and regeneration
- HER2/EGFR in Cancer Research
- Neuroscience and Neuropharmacology Research
- T-cell and B-cell Immunology
- Cardiovascular Function and Risk Factors
University of Hong Kong
2013-2025
Chinese University of Hong Kong
1985-2024
Queen Mary Hospital
1999-2023
Australia and New Zealand Banking Group
2023
York Teaching Hospital NHS Foundation Trust
2020
University of Nottingham
2014
Queen's Medical Centre
2014
University College Hospital
2012
University College London
2012
Sandwell & West Birmingham Hospitals NHS Trust
2012
Adenoviral vector-mediated gene transfer offers significant potential for therapy of many human diseases. However, progress has been slowed by several limitations. First, the insert capacity currently available adenoviral vectors is limited to 8 kb foreign DNA. Second, expression viral proteins in infected cells believed trigger a cellular immune response that results inflammation and only transient transferred gene. We report development new vector all coding sequences removed. Thus, large...
Protein kinase C has been shown to be a phospholipid/Ca2+-dependent enzyme activated by diacylglycerol (Nishizuka, Y. (1984) Nature 308, 693-697; Nishizuka, Science 225, 1365-1370). We have reported that unsaturated fatty acids (oleic acid and arachidonic acid) can activate protein independently of Ca2+ phospholipid (Murakami, K., Routtenberg, A. (1985) FEBS Lett. 192, 189-193). This study shows other cis-fatty such as linoleic also fully in the same manner. None saturated (C:4 C:18) nor...
Although heart failure with a preserved or normal ejection fraction (HFNEF diastolic failure) is common, treatment outcomes on quality of life and cardiac function are lacking. The effect renin-angiotensin blockade by irbesartan ramipril in combination diuretics (QoL), regional global systolic was assessed HFNEF patients.150 patients (LVEF >45%) were randomised to (1) alone, (2) plus irbesartan, (3) ramipril. QoL, 6-minute walk test (6MWT) Doppler echocardiography performed at baseline, 12,...
Left ventricular diastolic dysfunction is common in patients with systolic heart failure and the restrictive type of filling pattern appears to be associated increased cardiac mortality. Both artrial brain (or ventricular) natriuretic peptides are also proven markers severity failure. The aim this study was determine a large cohort whether abnormalities, particular transmitral flow velocity, correlate plasma atrial peptide levels.Sixty-eight consecutive symptomatic (ejection fraction < 0.5)...
To examine the role of Epstein-Barr virus (EBV) in lymphoepithelial carcinoma salivary gland Hong Kong Chinese.Ten cases (eight parotid and two submandibular) were examined. In situ hybridisation was used to localise EBER RNA, immunohistochemical methods detect expression latent membrane protein 1 (LMP-1) EBV positive tumours, Southern blot analysis clonality where frozen tissue available.None had a history Sjögren's syndrome or histological evidence benign lesion. The IgA antibody titre...
The association of Epstein-Barr virus (EBV) with lymphoepithelioma-like carcinoma in the nasopharynx, which is common Chinese from southern region, well established. Recently, EBV has also been found to be associated carcinomas (LELCs) and prominent lymphoid infiltrates stomach. We investigated for presence 74 cases gastric adenocarcinoma 36 colorectal patients by situ hybridization (ISH) using an antisense EBER probe. In seven (9.5%) carcinoma, was highly expressed cells metastatic tumor...
The hair growth cycle consists of three stages known as the anagen (growing), catagen (involution), and telogen (resting) phases. This cyclical is regulated by a diversity factors. Although normal expression both epidermal factor its receptor (EGFR) in outer root sheath down-regulated with completion follicular growth, here we show that continuous follicles transgenic mice arrested development at final stage morphogenesis. Data from immunoprecipitation immunoblotting showed signals through...
To study the role of protein kinase C (PKC) and its substrates in neuronal function, we have investigated vitro endogenous phosphorylation phosphoprotein F1 after induction synaptic plasticity by long-term potentiation (LTP). The was found to increase 5 min (Routtenberg et al., 1985), 1 hr (Lovinger 1986) 3 d 1985) LTP. characteristics this bear close similarities a number proteins characterized various systems, such as B50 (brain specific, synaptosome-enriched protein), pp46 (a growth cone...
The G-protein coupled receptor (GPCR), Cysteine (C)-X-C Receptor 4 (CXCR4), plays an important role in prostate cancer metastasis. CXCR4 is generally regarded as a plasma membrane where it transmits signals that support transformation, progression and eventual Due to the central of tumorigenesis, therapeutics approaches such antagonist monoclonal antibodies have focused on receptors exist membrane. An emerging concept for they may localize associate with nucleus retain function mediate...
Background Dravet syndrome is a severe form of epilepsy. Majority patients have mutation in SCN1A gene, which encodes voltage-gated sodium channel. A recent study has demonstrated that 16% SCN1A-negative PCDH19, the gene encoding protocadherin-19. Mutations other genes account for only very small proportion families. TSPYL4 novel candidate within locus 6q16.3-q22.31 identified by linkage study. Objective The present examined mutations epileptic Chinese children with emphasis on syndrome....
The in vivo role of epidermal growth factor (EGF) is not well defined even though its effects on culture cells were studied. To understand the developmental, physiological, and pathological roles EGF, we have generated transgenic mice widely expressing human EGF with use beta-actin promoter. transforming alpha (TGFalpha) bind equal affinity to receptor, a transmembrane tyrosine kinase, trigger various biological responses. TGFalpha signaling are implicated development reproductive system....
Objective Insufficiency of mannose-binding lectin (MBL) is associated with recurrent infections. Rheumatoid arthritis (RA) may represent an aberrant immune response to This study examined the phenotypic expression and variant alleles MBL gene its etiologic role in Chinese RA. Methods We studied 211 RA patients 196 healthy subjects. Serum concentrations codon-54 mutation promoter polymorphisms were analyzed. Clinical characteristics disease activity also examined. Results Patients had...
The epidermal growth factor (EGF) family of peptides signals through the erbB receptor tyrosine kinases and plays important roles in development tumorigenesis. Both EGF transforming (TGF)-α only bind to erbB1 activate it. precursor is distinct from that TGF-α having eight additional EGF-like repeats. We have recently shown without these repeats biologically active leads hypospermatogenesis transgenic mice. Here we present evidence mice widely expressing this engineered also stunted. These...
Epstein-Barr virus (EBV) is strongly associated with nasopharyngeal carcinoma and lymphoepithelioma-like carcinomas (LELC) of foregut-derived organs. Recently this group EBV-associated has been expanded by the identification in conventional adenocarcinomas stomach. In situ hybridization (ISH) using a sensitive digoxigenin-labelled EBER RNA probe was performed on 167 consecutive unselected primary non-small cell lung carcinomas, to determine frequency EBV association these tumours. Nine cases...
Testicular size, semen characteristics and serum reproductive hormone levels were studied in 1239 normal Chinese men. The mean testicular size of 17 ml this group subjects was similar to other reports Orientals but smaller than that reported Caucasians. Semen volume, the sperm count, motility morphology comparable those described for Caucasian subjects. cervical mucus penetration test (Kremer's test) zona‐free hamster egg also some Age showed a positive correlation with FSH LH, which highly...
The recent discovery of the cancer-associated E76K mutation in histone H2B (H2BE76-to-K) several types cancers revealed a new class oncohistone. H2BE76K weakens stability octamers, alters gene expression, and promotes colony formation. However, mechanism linking to cancer development remains largely unknown. In this study, we knock MDA-MB-231 breast cells using CRISPR/Cas9 show that mutant preferentially localizes genic regions. Interestingly, genes upregulated are enriched for involved cell...
SUMMARY The aim of this study was to determine the developmental profile mannose-binding protein (MBP) in preterm infants. MBP measured 885 longitudinally collected serum samples from 168 infants, and 63 were genotyped with respect codon 54 mutation gene. level/codon genotyping also determined on cord blood 146/123 term infants 138/123 adults, respectively. best cut-off values for dividing preterm, adults into ‘low’ ‘high’ M BP groups 400 ng/ml (55 low, 113 high), 700 (35 111 high) 750 (33...