A5052844897- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Insect Utilization and Effects
- Olfactory and Sensory Function Studies
- Animal Behavior and Reproduction
- Physiological and biochemical adaptations
- Insect and Pesticide Research
- Circadian rhythm and melatonin
- Plant and animal studies
- Biochemical Analysis and Sensing Techniques
- Insect Pheromone Research and Control
- Insect Pest Control Strategies
- Insect-Plant Interactions and Control
- Insect Resistance and Genetics
- Invertebrate Immune Response Mechanisms
- Biochemical and Molecular Research
- Research Data Management Practices
- Biochemical Acid Research Studies
- Digital and Traditional Archives Management
- Sociology and Education Studies
- Yersinia bacterium, plague, ectoparasites research
- Fossil Insects in Amber
- Big Data Technologies and Applications
- Receptor Mechanisms and Signaling
- Cell Image Analysis Techniques
Philipps University of Marburg
2010-2023
Clausthal University of Technology
2020-2023
Swedish University of Agricultural Sciences
2013
Universidad CES
2005-2009
Zero to Three
2001
Technical University of Munich
1993-1995
Neuropeptides and protein hormones are ancient molecules that mediate cell-to-cell communication. The whole genome sequence from the red flour beetle Tribolium castaneum , along with those other insect species, provides an opportunity to study evolution of genes encoding neuropeptide hormones. We identified 41 these in by using a combination bioinformatic peptidomic approaches. These encode >80 mature neuropeptides hormones, 49 peptides which were experimentally peptidomics central...
Neuropeptides and their G protein-coupled receptors (GPCRs) play a central role in the physiology of insects. One large family insect neuropeptides are adipokinetic hormones (AKHs), which mobilize lipids carbohydrates from fat body. Other peptides corazonins that structurally related to AKHs but represent different neuropeptide signaling system. We have previously cloned an orphan GPCR malaria mosquito Anopheles gambiae was intermediate between A. AKH corazonin GPCRs. Using functional...
Abstract The fruitfly, Drosophila , is dependent on its olfactory sense in food search and reproduction. Processing of odorant information takes place the antennal lobes, primary center insect brain. Besides classical neurotransmitters, earlier studies have indicated presence a few neuropeptides system. In present study we made an extensive analysis expression lobes by direct profiling using matrix‐assisted laser desorption/ionization‐time‐of‐flight (MALDI‐TOF) mass spectrometry...
Chemoreception is based on the senses of smell and taste that are crucial for animals to find new food sources, shelter, mates. The initial step in olfaction involves translocation odorants from periphery through aqueous lymph olfactory sensilla odorant receptors most likely by chemosensory proteins (CSPs) or binding (OBPs). To better understand roles CSPs OBPs a coleopteran pest species, red flour beetle Tribolium castaneum (Coleoptera, Tenebrionidae), we performed transcriptome analyses...
Lepidopterans like the giant sphinx moth Manduca sexta are known for their conspicuous sexual dimorphism in olfactory system, which is especially pronounced antennae and antennal lobe, primary integration center of odor information. Even minute scents female pheromone detected by male moths, facilitated a huge array receptors on antennae. The associated neuropilar areas glomeruli, enlarged males organized form so-called macroglomerular complex (MGC). In this study we searched anatomical more...
The red flour beetle Tribolium castaneum is emerging as a further standard insect model beside Drosophila. Its genome fully sequenced and it susceptible for genetic manipulations including RNA-interference. We use this to study adult brain development plasticity primarily with respect the olfactory system. In current study, we provide 3D atlases of freshly eclosed female male beetles (A0). include eight paired three unpaired neuropils antennal lobes (ALs), optic lobe neuropils, mushroom body...
The red flour beetle Tribolium castaneum is an emerging insect model organism representing the largest order, Coleoptera, which encompasses several serious agricultural and forest pests. Despite ecological economic importance of beetles, most olfaction studies have so far focused on dipteran, lepidopteran, or hymenopteran systems. Here, we present first detailed morphological description a coleopteran olfactory pathway in combination with genome-wide expression analysis relevant gene...
In many insect species olfaction is a key sensory modality. However, examination of the chemical ecology insects has focussed up to now on living above ground. Evidence for behavioral responses cues in soil other than CO2 scarce and role played by process finding host roots below ground not yet understood. The question whether soil-dwelling beetle larvae can smell their plant been under debate, but proof as lacking that olfactory perception volatile compounds released damaged plants, known...
We present a fast, nondestructive, and quantitative approach to characterize the morphology of capillary silica-based monolithic columns by reconstruction from confocal laser scanning microscopy images. The method comprises column pretreatment, image acquisition, processing, statistical analysis data. received morphological data are chord length distributions for bulk macropore space skeleton silica monolith. information is shown be comparable that derived transmission electron microscopy,...
Aedes aegypti is a model species in which the endogenous regulation of odor-mediated host seeking behavior has received some attention. Sugar feeding and female A. are transiently inhibited following blood meal. This inhibition partially mediated by short neuropeptide F (sNPF). The paired antennal lobes (ALs), as first processing centers for olfactory information, been shown to play significant role neuropeptidergic behaviors insects. expression sNPF, along with other peptides ALs aegypti,...
For many insects, including mosquitoes, olfaction is the dominant modality regulating their behavioral repertoire. Many neurochemicals modulate olfactory information in central nervous system, primary center of antennal lobe. The most diverse and versatile insect system are found neuropeptides. In present study, we analyzed neuropeptides lobe yellow fever mosquito, Aedes aegypti , a major vector arboviral diseases. Direct tissue profiling by matrix‐assisted laser desorption ionization...
Apicomplexan parasites contain a vestigial plastid called apicoplast which has been suggested to be site of [Fe–S] cluster biogenesis. Here we report the cloning lipoic acid synthase (LipA) from Toxoplasma gondii , well known protein. It is able complement LipA‐deficient Escherichia coli strain, clearly demonstrating that parasite protein functional LipA. The N‐terminus T. LipA unusual with respect an internal signal peptide preceding targeting domain. Nevertheless, it efficiently targets...
Toxin complex (Tc) proteins termed TcaABC, TcdAB, and TccABC with insecticidal activity are present in a variety of bacteria including the yersiniae. The tc gene sequences thirteen Yersinia strains were compared, revealing high degree order conservation, but also remarkable differences respect to pseudogenes, sequence variability duplications. Outside pathogenicity island (tc-PAI Ye ) Y. enterocolitica strain W22703, pseudogene (tccC2'/3') encoding homology TccC similarity tyrosine...
A multitude of potential neurotransmitters and neuromodulators, including peptides, have been detected in the antennal lobe (AL), first synaptic relay central olfactory pathway insect brain. However, functional role neuropeptides this system has yet to be revealed. An important prerequisite understanding is match functionally different cell types AL with their peptide profiles by using electrophysiological recordings combined immunocytochemical studies and/or single-cell mass spectrometry....
The antennal lobe is the primary processing center for olfactory information in insects. To understand further neural circuitry of this brain area, we have investigated distribution gamma-aminobutyric acid (GABA) and its colocalization with neuropeptides noctuid moth Heliothis virescens. Immunocytochemical experiments an antiserum against GABA showed a large number labeled somata lobe; these were located exclusively lateral cell cluster. Stained neurites innervating all antennal-lobe...
Abstract The paired antennal lobes (ALs) of the sphinx moth Manduca sexta serve as a well‐established model for studying development primary integration centers odor information in brain. To further reveal role neuropeptides during AL development, we have analyzed cellular distribution, developmental time course, and regulation neuropeptide M. allatotropin (Mas‐AT). On basis morphology appearance formation, seven major types Mas‐AT‐immunoreactive (ir) cells could be distinguished. Mas‐AT‐ir...