A5014184296- Gene Regulatory Network Analysis
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- bioluminescence and chemiluminescence research
- Biosensors and Analytical Detection
- Analytical Chemistry and Sensors
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Bio-sensing Technologies
- Bacterial Genetics and Biotechnology
- 3D Printing in Biomedical Research
- Bacteriophages and microbial interactions
- Hydrology and Watershed Management Studies
- CRISPR and Genetic Engineering
- Cancer Genomics and Diagnostics
- Cancer Research and Treatments
- Parasites and Host Interactions
- Parasitic Diseases Research and Treatment
- Characterization and Applications of Magnetic Nanoparticles
- Molecular Communication and Nanonetworks
- Advanced Chemical Sensor Technologies
- Bacterial biofilms and quorum sensing
- Parasite Biology and Host Interactions
- Electrochemical sensors and biosensors
- Scientific Computing and Data Management
University College London
2016-2024
Imperial College London
2013-2017
Synthetic biology designed cell-free biosensors are a promising new tool for the detection of clinically relevant biomarkers in infectious diseases. Here, we report that modular DNA-encoded biosensor protein expression systems can be used to measure bacterial biomarker Pseudomonas aeruginosa infection from human sputum samples. By optimizing system and sample extraction, demonstrate quorum sensing molecule 3-oxo-C12-HSL samples cystic fibrosis lungs quantitatively measured at nanomolar...
Abstract Parasitic diseases affect millions of people worldwide, causing debilitating illnesses and death. Rapid cost-effective approaches to detect parasites are needed, especially in resource-limited settings. A common signature parasitic is the release specific proteases by at multiple stages during their life cycles. To this end, we engineered several modular Escherichia coli Bacillus subtilis whole-cell-based biosensors which incorporate an interchangeable protease recognition motif...
Abstract Biological computing is a promising field with potential applications in biosafety, environmental monitoring, and personalized medicine. Here we present work on the design of bacterial computers using spatial patterning to process information form diffusible morphogen-like signals. We demonstrate, mathematically experimentally, that single, modular, colonies can perform simple digital logic, complex functions be built by combining multiple colonies, removing need for further genetic...
The measurement of gene expression using fluorescence markers has been a cornerstone synthetic biology for the past two decades. However, use arbitrary units limited usefulness these data many quantitative purposes. Calibration measurements from flow cytometry and plate reader spectrophotometry implemented previously, but tools are disjointed. Here we pull together, in some cases improve, extant methods into single software tool, written as package R statistical framework. workflow is...
Abstract Whole‐cell biosensors hold potential in a variety of industrial, medical, and environmental applications. These can be constructed through the repurposing bacterial sensing mechanisms, including common two‐component system (TCS). Here we report on construction range novel that are sensitive to acetoacetate, molecule plays number roles human health biology. based AtoSC TCS. An ordinary differential equation model describe action TCS was developed sensitivity analysis this used help...
Abstract Building computationally capable biological systems has long been an aim of synthetic biology. The potential utility biocomputing devices ranges from biosafety and environmental applications to diagnosis personalised medicine. Here we present work for the design bacterial computers which use spatial patterning process information. Our are composed colonies which, inspired by in embryo development, receive information form diffusible morphogen-like signals. A computation is encoded...
Abstract The measurement of gene expression using fluorescence markers has been a cornerstone synthetic biology for the last two decades. However, use arbitrary units limited usefulness this data many quantitative purposes. Calibration measurements from flow cytometry and plate reader spectrophotometry implemented previously but tools are disjointed. Here we pull together, in some cases improve, extant methods into single software tool, written as package R statistical framework. workflow is...
SynBioBrain: building biological computers from bacterial Using synthetic biology, we can now engineer bacteria into whole-cell biosensors where sensing, transduction and output occur within the living cell. Applications include detection of harmful environmental agents, bioprocess monitoring, detecting medically relevant biomarkers. Engineering microbial consortia allows biosensor information to be integrated processed in a distributed fashion. In this project, construct formed engineered...
Abstract Whole-cell biosensors hold potential in a variety of industrial, medical and environmental applications. These can be constructed through the repurposing bacterial sensing mechanisms, including common two-component system. Here we report on construction range novel that are sensitive to acetoacetate, molecule plays number roles human health biology. based AtoSC An ODE model describe action system was developed sensitivity analysis this used help inform biosensor design. The final...
ADVERTISEMENT RETURN TO ISSUEPREVCorrectionNEXTORIGINAL ARTICLEThis notice is a correctionCorrection to "FlopR: An Open Source Software Package for Calibration and Normalization of Plate Reader Flow Cytometry Data"Alex J. H. FedorecAlex FedorecMore by Alex Fedorechttp://orcid.org/0000-0003-0165-1705, Clare M. RobinsonClare RobinsonMore Robinsonhttp://orcid.org/0000-0003-3581-2810, Ke Yan WenKe WenMore Wenhttp://orcid.org/0000-0001-7449-2624, Chris P. Barnes*Chris BarnesMore...