A5074259713- Soil Carbon and Nitrogen Dynamics
- Microbial Community Ecology and Physiology
- Forest Ecology and Biodiversity Studies
- Genomics and Phylogenetic Studies
- Climate change and permafrost
- Coal and Its By-products
- Molecular Biology Techniques and Applications
- Mycorrhizal Fungi and Plant Interactions
- Heavy metals in environment
- Methane Hydrates and Related Phenomena
- Cryospheric studies and observations
- Lichen and fungal ecology
- Plant-Microbe Interactions and Immunity
- Peatlands and Wetlands Ecology
- Legume Nitrogen Fixing Symbiosis
- Nematode management and characterization studies
- Toxic Organic Pollutants Impact
- Marine Toxins and Detection Methods
- Marine and coastal ecosystems
- Polar Research and Ecology
- Biofuel production and bioconversion
- Marine and environmental studies
- Indigenous Studies and Ecology
- Microbial bioremediation and biosurfactants
- Protist diversity and phylogeny
University of Copenhagen
2014-2021
Aarhus University
2017-2021
Geological Survey of Denmark and Greenland
2017-2020
Romanian Academy
2019
Recirculation of wood ash from energy production to forest soil improves the sustainability this form as recycled contains nutrients that otherwise would be lost at harvest. In addition, wood-ash is beneficial many soils due its inherent acid-neutralizing capabilities. However, has several ecosystem-perturbing effects like increased pH and pore water electrical conductivity both known strongly impact bacterial numbers community composition. Studies investigating responses application remain...
ABSTRACT Recycling of wood ash from energy production may counteract soil acidification and return essential nutrients to soils. However, amendment affects physicochemical parameters that control composition functional expression the microbial community. Here, we applied total RNA sequencing simultaneously assess impact on active communities genes all taxa. Wood significantly affected taxonomic (rRNA) as well (mRNA) profiles both agricultural forest soil. Increase in pH, electrical...
Abstract Ash from combustion of biofuels, for example wood chips, is often deposited as waste, but due to its high content essential plant nutrients and alkalinity, it has been proposed recycle ash a fertilizer liming agent in biofuel production forest. However, current legislation sets rather strict limitations wood‐ash application systems. The soil microfood web, that microorganisms their microfaunal grazers, protozoa nematodes, pivotal ecosystem processes such decomposition nutrient...
Summary Thawing permafrost can stimulate microbial activity, leading to faster decomposition of formerly preserved organic matter and CO 2 release. Detailed knowledge about the vertical distribution responsible community that is changing with increasing soil depth limited. In this study, we determined composition from cores sampled in a high Arctic heath at Svalbard, Norway; spanning active layer (AL) into (PL). A special aim has been on identifying recently thawed soil, transition zone...
Metatranscriptomics has been used widely for investigation and quantification of microbial communities' activity in response to external stimuli. By assessing the genes expressed, metatranscriptomics provides an understanding interactions between different major functional guilds environment. Here, we present a de novo assembly-based Comparative Workflow (CoMW) implemented modular, reproducible structure. typically uses short sequence reads, which can either be directly aligned reference...
Abstract Decomposition of soil organic matter (SOM) in permafrost terrain and the production greenhouse gases is a key factor for understanding climate change‐carbon feedbacks. Previous studies have shown that SOM decomposition mostly controlled by temperature, moisture, carbon‐nitrogen ratio (C:N). However, focus has generally been on site‐specific processes little known about variations controls across Arctic sites. For assessing decomposition, we retrieved 241 samples from 101 profiles...
Abstract Wood ash is alkaline and contains base-cations. Application of wood to forests therefore counteracts soil acidification recycle nutrients removed during harvest. application leads strong vertical gradients in physicochemical parameters. Consequently, we designed an experimental system where small-scale changes properties prokaryotic community structure could be followed after application. A mixed fly bottom was applied dosages 3 9 t ha −1 the surface mesocosms, simulating a typical...
Abstract Drilling and handling of permanently frozen soil cores without microbial contamination is concern because e.g. from the active layer above may lead to incorrect interpretation results in experiments investigating potential actual activity these low biomass environments. Here, we present an example how affected analysis potentially community permafrost soil. We also development use two tracers: (1) fluorescent plastic microspheres (2) Pseudomonas putida genetically tagged with Green...
Freshwater woloszynskioid dinoflagellates were collected independently in Scotland and Portugal found to belong a previously unknown species of the genus Borghiella, here described as B. andersenii. The new differs morphology nuclear-encoded LSU rDNA ITS sequences from dodgei tenuissima, two presently comprising Borghiella. Unusual features observed particularly during asexual reproduction, which took place motile stage – many other or so-called division cyst, recalling cell family...
Soil pH is probably the most important variable explaining bacterial richness and community composition locally as well globally. In contrast, effects on fungi appear to be less pronounced, but also studied. Here we analyze responses of bacteria in parallel over a local extreme gradient ranging from 4 8. We established by applying strongly alkaline wood ash dosages 0, 3, 9, 15, 30, 90 t ha –1 replicated plots Picea abies plantation assessed fungal using high throughput amplicon sequencing 1...
Abstract Background Metatranscriptomics has been used widely for investigation and quantification of microbial communities’ activity in response to external stimuli. By assessing the genes expressed, metatranscriptomics provide an understanding interactions between different major functional guilds environment. Here, we present de-novo assembly-based Comparative Workflow (CoMW) implemented a modular, reproducible structure, significantly improving annotation metatranscriptomes. typically...
Abstract Recycling of wood ash from energy production may counteract soil acidification and return essential nutrients to soils. However, amendment affects physicochemical parameters that control composition functional expression the microbial community. Here, we applied Total RNA-sequencing simultaneously assess impact on active communities genes all taxa. Wood significantly affected taxonomic (rRNA) as well (mRNA) profiles both agricultural forest soil. Increase in pH, electrical...
This experimental procedure was designed to isolate total the RNA aiming obtain intact and high-quality integrity (RIN>7) for further next generation sequencing analysis. The protocol developed analyse environmental microbial active community providing a clear overview of transcriptional activity in specific time or after different condition exposition sets, such as investigation thermal adaptation heat-shock. is specifically extract total-RNA from water samples although it can be used...