A5100333216- Chromatin Remodeling and Cancer
- Cancer Mechanisms and Therapy
- Protein Degradation and Inhibitors
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- MicroRNA in disease regulation
- Heat shock proteins research
- Cancer, Stress, Anesthesia, and Immune Response
- Neuropeptides and Animal Physiology
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Cancer, Lipids, and Metabolism
- Circular RNAs in diseases
- Immune cells in cancer
- Machine Learning in Bioinformatics
- ATP Synthase and ATPases Research
- Axon Guidance and Neuronal Signaling
- Angiogenesis and VEGF in Cancer
- Research on Leishmaniasis Studies
- Chemokine receptors and signaling
- Mechanisms of cancer metastasis
- Cancer Genomics and Diagnostics
- Bioinformatics and Genomic Networks
Shandong First Medical University
2025
Shandong Tumor Hospital
2025
Dartmouth College
2019-2024
Nankai University
2023-2024
Hainan Medical University
2024
Sun Yat-sen University
2024
Children's Hospital of Zhejiang University
2023
Guang’anmen Hospital
2022-2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2014-2023
Middlebury College
2022-2023
Abstract Many proteins regulate the expression of genes by binding to specific regions encoded in genome 1 . Here we introduce a new data set RNA elements human that are recognized RNA-binding (RBPs), generated as part Encyclopedia DNA Elements (ENCODE) project phase III. This class regulatory functions only when transcribed into RNA, they serve sites for RBPs control post-transcriptional processes such splicing, cleavage and polyadenylation, editing, localization, stability translation...
Genes encoding subunits of SWI/SNF (BAF) chromatin remodelling complexes are collectively altered in over 20% human malignancies, but the mechanisms by which these alter to modulate transcription and cell fate poorly understood. Utilizing mouse embryonic fibroblast cancer line models, here we show via ChIP-seq biochemical assays that preferentially targeted distal lineage specific enhancers interact with p300 histone H3 lysine 27 acetylation. We identify a greater requirement for at typical...
Collectively, genes encoding subunits of the SWI/SNF (BAF) chromatin remodeling complex are mutated in 20% all human cancers, with SMARCA4 (BRG1) subunit being one most frequently mutated. The modulates through activity two mutually exclusive catalytic subunits, and SMARCA2 (BRM). Here, we show that a SMARCA2-containing residual underlies oncogenic mutant cancers. We demonstrate exists cell lines plays essential roles cellular proliferation. Further, using data from loss-of-function...
Bromodomain-containing protein 9 (BRD9) is a recently identified subunit of SWI/SNF(BAF) chromatin remodeling complexes, yet its function poorly understood. Here, using genome-wide CRISPR-Cas9 screen, we show that BRD9 specific vulnerability in pediatric malignant rhabdoid tumors (RTs), which are driven by inactivation the SMARCB1 SWI/SNF. We find exists unique SWI/SNF sub-complex lacks SMARCB1, has been considered core subunit. While SMARCB1-containing complexes bound preferentially at...
Heat shock protein 90-alpha (Hsp90alpha) is an intracellular molecular chaperone. However, it can also be secreted with the underlying regulatory mechanism remaining far from clear. Here we show that Hsp90alpha a C-terminal truncated form and its secretion regulated by EEVD motif via interacting proteins containing tetratricopeptide repeat domains. We demonstrate of determined phosphorylation status at residue Thr-90, kinase A phosphatase 5. further prerequisite for proinvasiveness function...
Abstract Purpose: Elevated numbers of tumor-associated macrophages (TAM) in the tumor microenvironment are often correlated with poor prognosis melanoma. However, mechanisms by which TAMs modulate melanoma growth still poorly understood. This study was aimed at examining function and mechanism TAM-derived adrenomedullin (ADM) angiogenesis growth. Experimental Design: We established vitro vivo models to investigate relationship between ADM melanoma, role TAM-induced The clinical significance...
Abstract SWI/SNF chromatin remodeling complexes are pleomorphic multisubunit cellular machines that utilize the energy of ATP hydrolysis to modulate structure. The interact with transcription factors at promoters and enhancers gene expression contribute lineage specification, differentiation, development. Initial clues a role in tumor suppression for came over decade ago when encoding SMARCB1/SNF5 core subunit was found specifically inactivated nearly all pediatric rhabdoid tumors. In last...
Hormonal vitamin D, 1,25-dihydroxyvitamin D (1,25D), signals through the nuclear receptor (VDR). 1,25D regulates cell proliferation and differentiation has been identified as a cancer chemopreventive agent. FoxO proteins are transcription factors that control survival. They function tumor suppressors associated with longevity in several organisms. Accumulating data have revealed regulate similarly common target genes. We show here ligand-bound VDR posttranslational modification of proteins....
Endostatin (ES) was reported to stimulate apoptosis in endothelial cells, but the exact mechanism remains controversial. In present study, we elucidate of ES-induced cell apoptosis. Our results indicate that ES induces cytochrome c release and caspase-9 activation human microvascular cells (HMECs) at concentration 1 microM for 24 h, which initiates process. Further, ATP production, mitochondrial membrane potential, tubule formation assays showed promotes permeability transition pore (mPTP)...
Heat shock protein 90α (Hsp90α) is a ubiquitously expressed molecular chaperone that essential for eukaryotic homeostasis. Hsp90α can also be secreted extracellularly, where it has been shown to involved in tumor metastasis. Extracellular interacts with and promotes the proteolytic activity of matrix metalloproteinase-2 (MMP-2). However, regulatory mechanism on MMP-2 still unknown. Here we show stabilizes protects from degradation cells. Further investigation reveals this stabilization...
Lymphangiogenesis, the growth of lymphatic vessels, contributes to metastasis. However, precise mechanism underlying lymphangiogenesis remains poorly understood. This study aimed examine chemokine/chemokine receptors that directly contribute chemoattraction activated endothelial cells (LEC) and tumor lymphangiogenesis.We used quantitative RT-PCR analyze specifically expressed chemokine in LECs upon stimulation vascular factor-C (VEGF-C). Subsequently, we established vitro vivo models show...
Malignant rhabdoid tumors (MRT) are highly aggressive pediatric cancers that respond poorly to current therapies. In this study, we screened several MRT cell lines with large-scale RNAi, CRISPR-Cas9, and small-molecule libraries identify potential drug targets specific for these cancers. We discovered MDM2 MDM4, the canonical negative regulators of p53, as significant vulnerabilities. Using two compounds currently in clinical development, idasanutlin (MDM2-specific) ATSP-7041 (MDM2/4-dual),...
Abstract Endostatin has potent anti‐endothelial and anti‐angiogenic functions. was reported to reduce lymphangiogenesis by down‐regulating the level of VEGF‐C in tumour tissues. However, there is little evidence for direct function endostatin on lymphangiogenic endothelial cells vessels. Here, we report that cell surface nucleolin, which as an receptor mediating its anti‐tumour functions, also selectively expressed both vitro vivo . Treatment primary mouse lymphatic (mLECs) inhibits mLEC...
Accumulating evidence demonstrated that Hippo signaling pathway is implicated in tumor initiation and progression. However, there have been limited studies on establishing signatures utilizing genes related to the evaluate prognosis clinical efficacy colorectal cancer (CRC) patients. pathway-associated with prognostic significance were identified TCGA database. Subsequently, a signature associated was constructed using Cox LASSO regression analyses. Survival analysis, ROC stratified analyses...