A5034365173- DNA Repair Mechanisms
- Fungal and yeast genetics research
- Genomics and Chromatin Dynamics
- Bacterial Genetics and Biotechnology
- Microtubule and mitosis dynamics
- HIV Research and Treatment
- CRISPR and Genetic Engineering
- Plant Molecular Biology Research
- RNA and protein synthesis mechanisms
- Epilepsy research and treatment
- Child and Adolescent Health
- Health Sciences Research and Education
- Monoclonal and Polyclonal Antibodies Research
- Mental Health Research Topics
- Functional Brain Connectivity Studies
- Plant Reproductive Biology
- Research on Leishmaniasis Studies
- RNA Research and Splicing
- Food Security and Health in Diverse Populations
- Animal Genetics and Reproduction
- Cancer-related gene regulation
- Neuroscience and Neuropharmacology Research
- HIV/AIDS drug development and treatment
- Innovations in Medical Education
- HIV/AIDS Research and Interventions
Rutgers, The State University of New Jersey
2002-2022
Rutgers New Jersey Medical School
1999-2020
Brandeis University
2001
SickKids Foundation
1999
Hospital for Sick Children
1999
University of California, San Francisco
1985-1988
Abstract The RV144 Thai trial HIV-1 vaccine of recombinant poxvirus (ALVAC) and gp120 subtype B/subtype E (B/E) proteins demonstrated 31% efficacy. Here we design an ALVAC/Pentavalent B/E/E/E/E to increase the diversity motifs in immunogen elicit a broader antibody response enhance protection. We find that immunization rhesus macaques with pentavalent results protection 55% pentavalent-vaccine-immunized from simian–human immunodeficiency virus (SHIV) challenge. Systems serology responses...
ARS307 is highly active as a replication origin in its native location on chromosome III of Saccharomyces cerevisiae. Its ability to confer autonomous activity plasmids requires the presence an 11-bp autonomously replicating sequence (ARS) consensus (ACS), which also required for chromosomal function, well approximately 100 bp flanking ACS called domain B. To further define sequences ARS linker substitution mutagenesis B was carried out. The mutations defined two sequences, B1 and B2, that...
Autonomously replicating sequence (ARS) elements, which function as the cis-acting chromosomal replicators in yeast Saccharomyces cerevisiae , depend upon an essential copy of 11-bp ARS consensus (ACS) for activity. Analysis chromosome III replicator ARS309 unexpectedly revealed that its ACS differs from canonical at two positions. One changes observed inactivates other elements. This atypical binds origin recognition complex efficiently and is required replication Comparison with regions...
Eukaryotic chromosomes contain long linear DNA molecules that initiate replication at multiple sites. Although the chromosomal of yeast Saccharomyces cerevisiae are two to three orders magnitude smaller than those multicellular eu-karyotes, their pattern is similar, with active origins spaced approximately 40-kb intervals (for review, see Newlon 1988) and a regular temporal order Fangman Brewer 1991). An important advantage in study ability identify potential by simple plasmid assay. These...
Without the RAD51 strand exchange protein, Saccharomyces cerevisiae cannot repair a double-strand break (DSB) by gene conversion. However, cells can DSBs recombination-dependent, break-induced replication (BIR). -independent BIR is initiated more than 13 kb from DSB. Repair depends on 200-bp sequence adjacent to ARS310 , located ∼34 centromere-proximal DSB, but does not depend origin activity of . We conclude that ability recombination-induced fork copy >130 end chromosome special site...
In eukaryotic chromosomes, DNA replication initiates at multiple origins. Large inter-origin gaps arise when several adjacent origins fail to fire. Little is known about how cells cope with this situation. We created a derivative of Saccharomyces cerevisiae chromosome III lacking all efficient origins, the 5ORIΔ-ΔR fragment, as model for chromosomes large gaps. used construct in modified synthetic genetic array screen identify genes whose products facilitate long Genes identified are...
AbstractWhile many of the proteins involved in initiation DNA replication are conserved between yeasts and metazoans, structure origins themselves has appeared to be different. As typified by ARS1, Saccharomyces cerevisiae <150 bp long have a simple modular structure, consisting single binding site for origin recognition complex, initiator protein, one or more accessory sequences. initiates from discrete site. While important sequences currently less well defined, metazoan appear These large...
Saccharomyces cerevisiae chromosome III encodes 11 autonomously replicating sequence (ARS) elements that function as chromosomal replicators. The essential 11-bp ARS consensus (ACS) binds the origin recognition complex (ORC) has been experimentally defined for most of these replicators but not ARS318 (HMR-I), which is one HMR silencers. In this study, we performed a comprehensive linker scan analysis ARS318. Unexpectedly, replicator depends on 9/11-bp match to ACS positions ORC binding site...
ARS307 is highly active as a replication origin in its native location on chromosome III of Saccharomyces cerevisiae. Its ability to confer autonomous activity plasmids requires the presence an 11-bp autonomously replicating sequence (ARS) consensus (ACS), which also required for chromosomal function, well approximately 100 bp flanking ACS called domain B. To further define sequences ARS linker substitution mutagenesis B was carried out. The mutations defined two sequences, B1 and B2, that...
ARS elements of Saccharomyces cerevisiae are the cis-acting sequences required for initiation chromosomal DNA replication. Comparisons unrelated from different regions genome have revealed no significant sequence conservation. We compared seven pairs homologous two species, S. and carlsbergensis. In all but one case, ARS308-ARS308(carl) pair, blocks homology were detected. cases ARS305, ARS307, ARS309, previously identified functional found to be conserved in their carlsbergensis homologs....
Long gaps between active replication origins probably occur frequently during chromosome replication, but little is known about how cells cope with them. To address this issue, we deleted from S. cerevisiae III to create chromosomes long interorigin and identified mutations that destabilize them [originless fragment maintenance (Ofm) mutations]. ofm6-1 an allele of HST3, a sirtuin deacetylates histone H3K56Ac. Hst3p Hst4p are closely related, hst4Δ does not cause Ofm phenotype. Expressing...
The S-phase checkpoint activated at replication forks coordinates DNA when stall because of damage or low deoxyribonucleotide triphosphate pools. We explore the involvement in coordinating using dun1Δ cells that have a defect number stalled formed from early origins and are dependent on Chk1p pathway for survival is stalled. show providing additional S phase establishing paused fork pause site restores signaling to chk1Δ relieves reliance pathway. Origin licensing activation controlled by...
Eukaryotic chromosomes are duplicated during S phase and transmitted to progeny mitosis with high fidelity. Chromosome duplication is controlled at the level of replication initiation, which occurs cis-acting replicator sequences that spaced intervals approximately 40 kb along budding yeast Saccharomyces cerevisiae. Surprisingly, we found derivatives chromosome III lack known replicators were replicated segregated properly in least 96% cell divisions. To gain insight into mechanisms maintain...
Abstract DNA replication origins, specified by ARS elements in Saccharomyces cerevisiae, play an essential role the stable transmission of chromosomes. Little is known about evolution elements. We have isolated and characterized from a chromosome III recovered alloploid Carlsberg brewing yeast that has diverged its S. cerevisiae homeologue. The positions seven identified this carlsbergensis are conserved: they located intergenic regions flanked open reading frames homologous to those flank...
The engrailed gene (en) is a regulator of pattern formation in Drosophila melanogaster. Here, we summarize immunofluorescence studies that reveal an elaborate spatial and temporal program expression the en product early embryo. These results suggest controlled by number other genes. Likely candidates for these genes are known pattern-forming loci. We propose large proportion extensive locus (70 kb) contains target sequences such controlling products. also present which demonstrate encodes...
OPEN ACCESSJuly 20, 2020Pharmacology, Pharmacotherapy, and Pharmacopolicy Through an Evidence-Based Medicine: A Novel Approach for First-Year Medical Students Alexander M. Mozeika, PharmD, Rijul Asri, James F. Theis, PhD, Carolyn K. Suzuki, PhD PharmD https://orcid.org/0000-0003-4037-7526 Fourth-Year Student, Department of Education, Rutgers New Jersey School E-mail Address: [email protected] Google Scholar More articles by this author , Asri Adjunct Assistant Professor, Biochemistry &...
Passive transfer of monoclonal antibodies (mAbs) human origin into Non-Human Primates (NHPs), especially those which function predominantly by a Fc-effector mechanism, requires an priori preparation step, in the mAb is reengineered to equivalent NHP IgG subclass. This can be achieved changing both Fc and Fab sequence while simultaneously maintaining epitope specificity parent antibody. Ab reengineering process, referred as rhesusization, challenging because simple grafting complementarity...