A5049635622- Gene Regulatory Network Analysis
- Congenital Diaphragmatic Hernia Studies
- Acute Myeloid Leukemia Research
- Skin Protection and Aging
- Neonatal Respiratory Health Research
- Hematopoietic Stem Cell Transplantation
- Biochemical and Molecular Research
- Mesenchymal stem cell research
- Cytomegalovirus and herpesvirus research
- Laser Applications in Dentistry and Medicine
- Adenosine and Purinergic Signaling
- Cell Image Analysis Techniques
- DNA Repair Mechanisms
- Bioinformatics and Genomic Networks
- Genetics, Aging, and Longevity in Model Organisms
- Lung Cancer Research Studies
- Bone fractures and treatments
- Tissue Engineering and Regenerative Medicine
- Neuroendocrine Tumor Research Advances
- Circadian rhythm and melatonin
- Advanced Breast Cancer Therapies
- Clostridium difficile and Clostridium perfringens research
- Molecular spectroscopy and chirality
- bioluminescence and chemiluminescence research
- Ubiquitin and proteasome pathways
Universität Ulm
2015-2024
Institute of Molecular Medicine
2024
Whether aged hematopoietic stem and progenitor cells (HSPCs) have impaired DNA damage repair is controversial. Using a combination of mutation indicator assays, we observe 2- to 3-fold increase in the number mutations system upon aging. Young (HSCs) (HPCs) do not show an irradiation-induced repair, young HSPCs respond very similarly with respect cell-cycle checkpoint activation apoptosis. Both DNA-damage-induced G1-S checkpoint. Induction chronic double-strand breaks by zinc-finger nucleases...
Aging is associated with a decline in the function of hematopoietic stem cells (HSCs). This HSC results reduced hematologic regenerative capacity and an increased incidence disorders. In general, aged HSCs show on average increase cell size lower frequency polar for protein polarity markers. The has been proposed to be tightly linked potential HSCs, small showing higher compared large HSCs. upon aging may are located within bone marrow (BM) distinct microenvironments called niches. These...
Abstract The distribution of biomolecules within cells changes upon aging and diseases. To quantitatively determine the spatial components inside cells, we built user-friendly open-source 3D-cell-image analysis platform Cell Det ection A nalysis I ntensity L ounge (CellDetail). algorithm CellDetail is based on concept dipole moment. provides quantitative values for polarity proteins Cdc42 Tubulin in young aged hematopoietic stem (HSCs). Septin form networks that are critical cell...
The D-type cyclins (CCND1, CCND2, and CCND3) in association with CDK4/6 are known drivers of cell cycle progression. We reported previously that inactivation FOXO1 confers growth arrest apoptosis B-ALL, partially mediated by subsequent depletion CCND3. Given the canonical MYC target CCND2 has been considered to play major role B-ALL proliferation, further investigation CCND3 transcription is warranted. In this study, we demonstrated essential for proliferation survival independent mutational...
Adult hematopoietic stem cells (HSCs) maintain tissue homeostasis and regenerative capacity of the system through self-renewal differentiation. Metabolism is recognized as an important regulatory entity controlling cells. As purine nucleotides are essential for metabolic functions, we analyzed role hypoxanthine guanine phosphoribosyl transferase (HPRT)-associated salvaging in HSCs. Here, demonstrate that progenitor (HSPCs) show a strong dependence on HPRT-associated salvaging. HSPCs with...
The spindle assembly checkpoint plays a pivotal role in preventing aneuploidy and transformation. Many studies demonstrate impairment of this cancer cells. While leukemia is frequently driven by transformed hematopoietic stem progenitor cells (HSPCs), the biology such primary not very well understood. Here, we reveal that fully functional murine and, to lesser extent, We show HSPCs arrest at prometaphase induce p53-dependent apoptosis upon prolonged treatment with anti-mitotic drugs....
Endogenously mobilized stem and progenitor cells (SPCs) or exogenously provided SPCs are thought to be beneficial for trauma therapy. However, still little is known about the synchronized dynamics of number in blood after severe injury parameters like cytokine profiles that correlate with these numbers. We determined hematopoietic cells, common myeloid progenitors, granulocyte-macrophage mesenchymal stem/stromal peripheral (PB) 0 3, 8, 24, 48, 120 h polytrauma individual patients (injury...
Bacterial protein toxins became valuable molecular tools for the targeted modulation of cell functions in experimental pharmacology and attractive therapeutics because their potent specific mode action human cells. C2IN-C3lim, a recombinant fusion toxin (~50 kDa) Rho-inhibiting C3lim from Clostridium (C.) limosum non-toxic portion C. botulinum C2 (C2IN), is selectively internalized into cytosol monocytic cells where specifically ADP-ribosylates Rho A -B, thereby inhibiting Rho-mediated...
Abstract Deviations from the common distribution of proteins within cells are linked to aging and diseases. Analysis tools determine quantitative changes in spatial components inside not well developed. To this end, we built open-source automated analysis platform Cell Detection Intensity Lounge (CellDetail) together with a graphical user interface (GUI). The algorithm CellDetail generates physics-based, determination distributions multiple single cell. For example, transforms previously...
Thoracic trauma is a major cause of mortality due to the associated inflammatory acute respiratory distress syndrome and morbidity impaired tissue regeneration. Trauma-induced lung inflammation characterized by early recruitment cells with pro- or anti-inflammatory activity lung. Therapeutic interventions reducing level may result in decreased damage improved healing recovery. Stem might be able improve outcome via immunomodulation enhancing regeneration.Here, we describe migratory dynamics...