A5082136384- CAR-T cell therapy research
- Chronic Lymphocytic Leukemia Research
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Biosimilars and Bioanalytical Methods
- Genomics and Phylogenetic Studies
- Lymphoma Diagnosis and Treatment
- Microbial Community Ecology and Physiology
- Epigenetics and DNA Methylation
- Immune Response and Inflammation
- Yersinia bacterium, plague, ectoparasites research
- Asthma and respiratory diseases
- Viral Infections and Vectors
- Environmental DNA in Biodiversity Studies
- Histone Deacetylase Inhibitors Research
- Microbial infections and disease research
- Leptospirosis research and findings
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Ruminant Nutrition and Digestive Physiology
- Bacteriophages and microbial interactions
- Cancer Immunotherapy and Biomarkers
Fred Hutch Cancer Center
2018-2024
Temple University
2015-2017
J. Craig Venter Institute
2010-2013
A combination of Sanger and 454 sequences small subunit rRNA loci were used to interrogate microbial diversity in the bovine rumen 12 cows consuming a forage diet. Observed bacterial species richness, based on V1–V3 region 16S gene, was between 1,903 2,432 species-level operational taxonomic units (OTUs) when 5,520 reads sampled per animal. Eighty percent OTUs dominated by members order Clostridiales, Bacteroidales, Erysipelotrichales unclassified TM7. Abundance Prevotella varied widely...
Abstract Memory T cells sustain effector T-cell production while self-renewing in reaction to persistent antigen; yet, excessive expansion reduces memory potential and impairs antitumor immunity. Epigenetic mechanisms are thought be important for balancing differentiation; however, the epigenetic regulator(s) underpinning this process remains unknown. Herein, we show that histone methyltransferase Ezh2 controls CD8 + precursor formation activity. activates Id3 silencing Id2 , Prdm1 Eomes...
The whole genome analysis of two strains the first intermediately pathogenic leptospiral species to be sequenced (Leptospira licerasiae VAR010 and MMD0835) provides insight into their potential deepens our understanding evolution. Comparative eight genomes shows existence a core comprising 1547 genes 452 conserved restricted infectious (including L. licerasiae) that are likely pathogenicity-related. Comparisons functional content suggests retains several proteins related nitrogen, amino acid...
Abstract Notch signaling regulates multiple helper CD4+ T cell programs. We have recently demonstrated that dendritic cells (DCs) expressing the ligand DLL4 are critical for eliciting alloreactive responses and induction of graft-versus-host disease in mice. However, human counterpart murine DLL4+ DCs has yet to be examined. report identification their role regulating Th1 Th17 differentiation. CD1c+ plasmacytoid (pDCs) from peripheral blood (PB) healthy donors did not express DLL4. In...
Whole genome sequencing of viruses and bacteriophages is often hindered because the need for large quantities genomic material. A method described that combines single plaque with an optimization Sequence Independent Single Primer Amplification (SISPA). This can be used de novo whole next-generation any cultivable virus without large-scale production viral stocks or purification using centrifugal techniques.A a variant 2009 pandemic H1N1 human Influenza was isolated amplified optimized SISPA...
BackgroundIn relapsed/refractory (R/R) chronic lymphocytic leukemia (CLL) patients (pts) who had previously failed ibrutinib, we observed durable responses after CD19 chimeric antigen receptor-modified T-cell immunotherapy (Turtle CJ et al, JCO, 2017; NCT01865617). The combination of CAR-T cells with ibrutinib might improve response and decrease toxicity.ObjectivesTo evaluate the efficacy toxicity cell alone or in for R/R CLL.MethodsOur phase 1/2 study established a preferred regimen CLL pts...
Introduction: Patients (pts) with follicular lymphoma early relapse after initial chemoimmunotherapy have limited survival, as do those who fail multiple regimens or develop histologic transformation to large cell non-Hodgkin (NHL). Initial responses CD19-directed chimeric antigen receptor (CAR)-modified T (CAR-T) immunotherapy in pts been reported; however, the incidence and durability of without (FL) (tFL) not established. Methods: We conducted a phase 1/2 clinical trial (NCT01865617) CD19...
IntroductionCD19 chimeric antigen receptor (CAR)-T cell therapy has shown efficacy in Non-Hodgkin lymphoma (NHL), but the factors associated with durable remission have not been identified.ObjectivesWe report multivariate analyses of impacting progression-free survival (PFS) NHL patients (pts) treated cyclophosphamide and fludarabine (Cy/Flu) lymphodepletion (LD) CD19 CAR-T cells.MethodsWe conducted a phase 1/2 open-label clinical trial (NCT01865617) primary objective evaluating feasibility...
Introduction: We previously reported that CD19-targeted chimeric antigen receptor-engineered T (CD19 CAR-T) cells could lead to durable responses in relapsed/refractory (R/R) chronic lymphocytic leukemia (CLL) patients (pts) who had failed ibrutinib therapy. Since pre-clinical studies showed might improve CAR-T cell anti-tumor efficacy and reduce cytokine release syndrome (CRS), we carried out a pilot study evaluate the safety of CD19 with concurrent administration. Methods: treated pts...