A5032211778- Mycorrhizal Fungi and Plant Interactions
- Plant Pathogens and Fungal Diseases
- Fungal Biology and Applications
- Lichen and fungal ecology
- Forest Ecology and Biodiversity Studies
- Genomics and Phylogenetic Studies
- Forest Insect Ecology and Management
- Yeasts and Rust Fungi Studies
- Microbial Community Ecology and Physiology
- Legume Nitrogen Fixing Symbiosis
- Nematode management and characterization studies
- Plant Pathogenic Bacteria Studies
- Plant and animal studies
- Ecology and Vegetation Dynamics Studies
- Plant and Fungal Interactions Research
- Enterobacteriaceae and Cronobacter Research
- Plant-Microbe Interactions and Immunity
- Genetic diversity and population structure
- Plant and Biological Electrophysiology Studies
- Plant and Fungal Species Descriptions
- Probiotics and Fermented Foods
- Fermentation and Sensory Analysis
- Banana Cultivation and Research
- Coastal wetland ecosystem dynamics
- Phytochemistry and biological activity of medicinal plants
University of Pretoria
2015-2024
JDSU (United States)
2024
Centro de Investigación y Extensión Forestal Andino Patagónico
2010
Stellenbosch University
2010
Plant (United States)
2010
University of Valparaíso
2010
North Eastern University
2010
Agricultural Biotechnology Institute
2003
Forestry and Agricultural Biotechnology Institute
2003
Robust molecular markers such as microsatellites are important tools used to understand the dynamics of natural populations, but their identification and development typically time consuming labor intensive. The recent emergence so-called next-generation sequencing raised question whether this new technology might be applied microsatellite development. Following view, we considered deep using 454 Life Sciences/Roche GS-FLX genome system could lead a rapid protocol develop primers for genetic...
Novel species of fungi described in the present study include following from South Africa: Alanphillipsia aloeicola Aloe sp., Arxiella dolichandrae Dolichandra unguiscati, Ganoderma austroafricanum Jacaranda mimosifolia, Phacidiella podocarpi and Phaeosphaeria Podocarpus latifolius, Phyllosticta mimusopisicola Mimusops zeyheri Sphaerulina pelargonii Pelargonium sp. Furthermore, Barssia maroccana is Cedrus atlantica (Morocco), Codinaea pini Pinus patula (Uganda), Crucellisporiopsis marquesiae...
The Capnodiales, which includes fungi known as the sooty moulds, represents second largest order in Dothideomycetes, encompassing morphologically and ecologically diverse with different lifestyles modes of nutrition. They include saprobes, plant human pathogens, mycoparasites, rock-inhabiting (RIF), lichenised, epi-, ecto- endophytes. aim this study was to elucidate evolutionary patterns Capnodiales well reconsider their phylogeny by including numerous new collections using four nuclear...
Background Gene conversion is the mechanism proposed to be responsible for homogenization of multigene families such as nuclear ribosomal gene clusters. This concerted evolutionary process prevents individual genes in clusters from accumulating mutations. The evolution not well understood but recombination during meiosis has been hypothesized play a significant role this homogenization. In study we tested hypothesis unequal crossing over playing events within RNA cistron meiosis, mitosis or...
Abstract Species of Armillaria are distributed globally and include some the most important pathogens forest ornamental trees. Some them form large long-living clones that considered as one largest organisms on earth capable long-range spore-mediated transfer well vegetative spread by drought-resistant hyphal cords called rhizomorphs. However, virus community infecting these species has remained unknown. In this study we used dsRNA screening high-throughput sequencing to search for possible...
Abstract Dead and dying oak ( Quercus ) numerous other woody ornamental trees shrubs showing signs symptoms of Armillaria root rot were identified in the Company Gardens, Cape Town, South Africa, which established mid‐1600s by Dutch East Indies Trading Company. Nineteen isolates from or mushrooms collected analysed to identify characterize sp. responsible for disease. The Alu I digestion amplified product first intergenic spacer region (IGS‐1) rRNA operon 19 Gardens was identical that some...
Some of the most significant breakthroughs in biological sciences this century will emerge from development next generation sequencing technologies. The ease availability DNA sequence made possible through these new technologies has given researchers opportunities to study organisms a manner that was not with Sanger sequencing. Scientists will, therefore, need embrace genomics, as well develop and nurture human capacity genomes utilise 'tsunami' data genome In response challenges, we...
Jacaranda mimosifolia trees have been progressively dying due to Ganoderma root and butt rot disease in Pretoria (the "City of Jacarandas") for many years. austroafricanum was described from these previously but this based on a single collection. This study treats substantially expanded collection isolates made all where basidiomes were present suburb. DNA sequences obtained the ITS LSU region compared against GenBank. Phylogenetic analyses used compare with those other species. Based...
The Erwiniaceae contain many species of agricultural and clinical importance. Although relationships among most the genera in this family are relatively well resolved, phylogenetic placement several taxa remains ambiguous. In study, we aimed to address these uncertainties by using a combination genomic approaches. Our multilocus sequence analysis genome-based maximum-likelihood phylogenies revealed that arsenate-reducing strain IMH plant-associated ATCC 700886, both previously presumptively...
Abstract Background Proteins in the Glycoside Hydrolase family 32 (GH32) are carbohydrate-active enzymes known as invertases that hydrolyse glycosidic bonds of complex saccharides. Fungi rely on these to gain access and utilize plant-derived sucrose. In fungi, GH32 invertase genes found higher copy numbers genomes pathogens when compared closely related saprophytes, suggesting an association between ecological strategy. The aim this study was investigate distribution evolution...
The genomes of Armillaria fuscipes, Ceratocystiopsis minuta, Ceratocystis adiposa, Endoconidiophora laricicola, E. polonica, and Pénicillium freii DAOMC 242723 are presented in this genome announcement. These six from plant pathogens otherwise economically important fungal species. sizes range 21 Mb the case minuta to 58 for basidiomycete fuscipes. include first reports genus Endoconidiophora. availability these data will provide opportunities resolve longstanding questions regarding...
This genome announcement includes draft genomes from Claviceps purpurea s.lat., including C. arundinis, humidiphila and cf. spartinae. The of Davidsoniella eucalypti, Quambalaria eucalypti Teratosphaeria destructans, all three important eucalyptus pathogens, are presented. insect associate Grosmannia galeiformis is also described. pine pathogen Fusarium circinatum has been assembled into pseudomolecules, based on additional sequence data by harnessing the known synteny within fujikuroi...
example, Sipos et al. (2017) showed that genome evolution in the genus was predominantly caused by gene family expansion.Kolesnikova (2019) assembled complete mitochondrial genomes of A. borealis, gallica, sinapina, and solidipes found a high degree variation size, content genomic organization among these phylogenetically closely related species.Recently, first chromosome-level Armillaria assembly became available, revealing genome-wide recombination ostoyae (Heinzelmann 2020).Sequenced...
Ganoderma is a cosmopolitan genus that encompasses species with cultural, economic, and pathogenic importance. Despite the importance of this genus, knowledge pertaining to diversity in South Africa limited. This study aimed at elucidating identity phylogenetic placements samples obtained during survey wood-rotting fungi Garden Route National Park (GRNP) Africa, supplemented isolates from other localities across country. Identification was achieved by means multilocus inference combined...
Armillaria mellea was once thought to be a morphologically variable species with wide distribution and very broad host range. The relatively recent development of an indirect assessment sexual incompatibility in has led identification biological within A. s. 1. Partial intergenic spacer region (IGS) sequences the ribosomal RNA (rRNA) operon have been determined for most Northern Hemisphere Armillaria, but not aim this study determine phylogenetic relationships among isolates based on DNA...
Cypress canker is a serious disease that has devastated Cupressus spp. in many parts of the world. In Mediterranean Europe it caused deaths millions trees. Three species Seiridium, S. cardinale, cupressi, and unicorne, are associated with cypress canker. Considerable debate surrounds taxonomic status these fungi. They have been viewed as single morphologically variable species, three distinct taxa; or two based on presence absence conidial appendages. Studies ribosomal DNA (ITS1, ITS2, 5.8S...